Skip to main content
SpringerLink
Log in

Stable degradation of benzoate byKlebsiella oxytoca C302 immobilized in alginate and polyurethane

  • Published:
Biotechnology and Bioprocess Engineering Aims and scope Submit manuscript

Abstract

Benzoate produced from the degradative pathways of various aromatic chemicals is generally recognized as a pollutant compound. However, various bacterial strains isolated as benzoate degraders have exhibited certain limits to their functions, including a loss of viability and degradability when cultivated in a broth medium for a longer time. Accordingly, immobilization techniques have been utilized to overcome such problems, and the current study examined the use of alginate and polyurethane for immobilizingKlebsiella oxytoca C302 to extend its viability and degradability of benzoate. The organism was well encapsulated by both matrices and the immobilized cells showed a high stability as regards their viability and degradability of 2 mM benzoate in a MM2 broth medium during cultivation for longer than 60 h in a semicontinuous batch system.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Assinder, S. J. and P. A. Williams (1990) The TOL plasmids: Determinants of the catabolism of toluene and the xylenes.Adv. Microbial Physiol. 31: 1–69.

    Article  CAS  Google Scholar 

  2. Furukawa, K. (1994) Molecular genetics and evolutionary relationship of PCB-degrading bacteria.Biodegradation 5: 89–300.

    Article  Google Scholar 

  3. Dietrich, D., W. J. Hichey, and R. Lamar (1995) Degradation of 4,4′-dichlorobiphenyl,3,3′,4,4′-tetrachlorobiphenyl, and 2,2′,4,4′,5,5′-hexatetra-chlorobiphenyl by the white rot fungusPhanerochaete chrysosporium.Appl. Environ. Microbiol. 61: 3904–3909.

    CAS  Google Scholar 

  4. Brenner, V., J. J. Arensdorf, and D. D. Focht (1994) Genetic construction of PCB degraders.Biodegradation 5: 359–377.

    Article  CAS  Google Scholar 

  5. Smith, M. R. and C. Ratledge (1989) Catabolism of biphenyl byPseudomonas sp. NCIB 10643 andNacardia sp. NCIB 10503.Appl. Microbiol Biotechnol. 30: 395–401.

    Article  CAS  Google Scholar 

  6. Nam, J. H. and C. K. Kim (1994) Cloning ofpcb genes inPseudomonas sp. P20 specifying degradation of 4-chlorobiphenyl.Kor. J. Appl. Microbiol. Biotechnol. 22: 353–359.

    Google Scholar 

  7. van Agteren, M. H., S. Keuning, and D. B. Janssen (1998)Handbook on Biodegradation and Biological Treatment of Hazardous Organic Compounds. Kluwer Acad. Publ. Netherlands.

    Google Scholar 

  8. Kim, K. P., J. S. Lee, S. I. Park, M. S. Rhee, K. S. Bae, and C. K. Kim (2000) Isolation and identification ofKlebsiella oxytoca C302 and its degradation of aromatic hydrocarbons.Kor. J. Microbiol. 36: 58–63.

    Google Scholar 

  9. Mohamed M. E., A. Zaar, C. Ebenau-Jehle, and G. Fuchs (2001) Reinvestigation of a new type of aerobic benzoate metabolism in the proteobacteriumAzoarcus evansii.J. Bacteriol. 183: 1899–908.

    Article  CAS  Google Scholar 

  10. Chae, J. C., K. J. Ahn, and C. K. Kim (1998) Hydrolytic dechlorination of 4-chlorobenzoate specified byfcbABC ofPseudomonas sp. DJ-12.J. Microbiol. Biotechnol. 8: 692–695.

    CAS  Google Scholar 

  11. Seo, D. I., J. C. Chae, K. P. Kim, Y. Kim, K. S. Lee, and C. K. Kim (1998) A pathway for 4-chlorobenzoate degradation byPseudomonas sp. S-47.J. Microbiol. Biotechnol. 8: 96–100.

    CAS  Google Scholar 

  12. Cassidy, M. B., H. Lee, and J. T. Trevors (1996) Environmental applications of immobilized microbial cells: a review.J. Ind. Microbiol. 16: 79–101.

    Article  CAS  Google Scholar 

  13. Mordocco, A., C. Kuek, and R. Jenkins (1999) Continuous degradation of phenol at low concentration using immobilizedPseudomonas putida.Enzyme Microb. Technol. 25: 530–536.

    Article  CAS  Google Scholar 

  14. Sahasrabudhe, S. R., A. J. Modi, and V. V. Modi (1998) Dehalogenation of 3-chlorobenzoate by immobilizedPseudomonas sp. B13 cells.Biotechnol. Bioeng. 31: 889–893.

    Article  Google Scholar 

  15. Manohar, S., C. K. Kim, and T. B. Karegoudar (1999) Production of salicylic acid from naphthalene by immobilizedPseudomonas sp. strain NGK1.J. Microbiol. Biotechnol. 9: 482–487.

    CAS  Google Scholar 

  16. Mukerjee-Dhar, G., M. Shiura, and K. Kimbara (1998) Degradation of polychlorinated biphenyl by cells ofRhodococcus opacus strain TSP203 immobilized in alginate and in solution.Enzyme Microb. Technol. 23: 34–41.

    Article  CAS  Google Scholar 

  17. Manohar, S., C. K. Kim, and T. B. Karegoudar (2001) Enhanced degradation of naphthalene by immobilization ofPseudomonas sp. strain NGK1 in polyurethane foam.Appl. Microbiol. Biotechnol. 55: 311–316.

    Article  CAS  Google Scholar 

  18. Arensdorf, J. J. and D. D. Focht (1995) A meta-cleavage pathway for 4-chlorobenzoate an intermediate in the metabolism of 4-chlorobiphenyl byPseudomonas cepacia P166.Appl. Environ. Microbiol. 61: 443–447.

    CAS  Google Scholar 

  19. Koo, S. M., Y. H. Cho, C. S. Huh, Y. J. Baek, and J. Y. Park (2001) Improvement of the stability ofLactobacillus casei YIT 9018 by microencapsulation using alginate and chitosan.J. Microbiol. Biotechnol. 11: 376–383.

    CAS  Google Scholar 

  20. Quintana, M. G. and H. Dalton (1999) Biotransformation of aromatic compounds by immobilized bacterial strains in barium alginate beads.Enzyme Microb. Technol. 24: 232–236.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Microbiology and Biotechnology, Chungbuk National University, 361-763, Cheongju, Korea

    Jun-Ho Kim & Chi Kyung Kim

  2. Biotechnology Research Institute, Chungbuk National University, 361-763, Cheongju, Korea

    Jun-Ho Kim & Chi Kyung Kim

  3. R & D Center, Boryung Pharmaceutical Co., 365-800, Jincheon, Korea

    Won-Hwa Jeong

  4. Department of Biochemistry, Gulbarga University, 585-106, Gulbarga, India

    T. B Karegoudar

Authors
  1. Jun-Ho Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Won-Hwa Jeong
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. T. B Karegoudar
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Chi Kyung Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Chi Kyung Kim.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kim, JH., Jeong, WH., Karegoudar, T.B. et al. Stable degradation of benzoate byKlebsiella oxytoca C302 immobilized in alginate and polyurethane. Biotechnol. Bioprocess Eng. 7, 347–351 (2002). https://doi.org/10.1007/BF02933519

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02933519

Keywords

Search

Navigation