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Whole-cell Immobilization of Engineered Escherichia coli JY001 with Barium-alginate for Itaconic Acid Production

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Abstract

Itaconic acid is an important organic acid and a major component of various polymers. It is used in resins, superabsorbent polymers, and substitutes for petrochemicalbased monomers such as acrylic and methacrylic acids. Itaconic acid is primarily produced by the fungus Aspergillus terreus, which yields a high titer with albeit long fermentation period and by-products. In our previous study, Escherichia coli JY001 was reported to produce itaconic acid using citric acid in whole-cell reaction resulting in higher itaconic acid productivity with less by-products formation. The present study aimed to increase whole-cell enzyme stability and reusability, via immobilization of E. coli JY001 using barium-alginate beads. We optimized the cations, temperature, pH, alginate, BaCl2 concentration, cell density per bead, and CTAB content to improve transfer rate of substrates and products. Under the optimized conditions, immobilized whole cells were stable for four repeated cycles of itaconic acid production. The present results would strengthen the basis for a continuous itaconic acid production.

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Acknowledgements

This study was supported by the National Research Foundation of Korea (NRF) (NRF-2015M1A5A1037196, NRF-2017R1D1A1B03033594), Research Program to solve social issues of the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (2017M3A9E4077234). This work was also supported by Polar Academic Program (PAP, PE18900).

Dr. Ranjit Gurav is thankful to Konkuk University, Korea for providing financial support under KU research professor program.

Consulting service from the Microbial Carbohydrate Resource Bank (MCRB, Seoul, Korea) was kindly appreciated.

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  1. The authors equally contributed to this study.

Authors and Affiliations

  1. Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, 05029, Korea

    Yu-Mi Moon, Ranjit Gurav, Junyoung Kim, Yun-Gi Hong, Shashi Kant Bhatia, Hye-Rim Jung, Ju-Won Hong, Tae Rim Choi, Soo Yeon Yang, Hyung Yeon Park & Yung-Hun Yang

  2. Institute for Ubiquitous Information Technology and Applications (CBRU), Konkuk University, Seoul, 05029, Korea

    Shashi Kant Bhatia

  3. Department of Biotechnology, College of Engineering, Duksung Women’s University, Seoul, 01369, Korea

    Hwang-Soo Joo

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  1. Yu-Mi Moon
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  2. Ranjit Gurav
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Correspondence to Hwang-Soo Joo or Yung-Hun Yang.

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Moon, YM., Gurav, R., Kim, J. et al. Whole-cell Immobilization of Engineered Escherichia coli JY001 with Barium-alginate for Itaconic Acid Production. Biotechnol Bioproc E 23, 442–447 (2018). https://doi.org/10.1007/s12257-018-0170-3

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  • DOI: https://doi.org/10.1007/s12257-018-0170-3

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