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Biodegradation of Phenol Using the Indigenous Rhodococcus pyridinivorans Strain PDB9T NS-1 Immobilized in Calcium Alginate Beads

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Abstract

Phenolic compounds are the major contaminants identified from various industrial effluents, which pose an extreme threat to the environment. Therefore, investigating an effective technique to remove these toxic phenolic compounds from the contaminated environment is very essential. In the present investigation, batch tests were performed to assess the biodegradation of phenol using an indigenous Rhodococcus pyridinivorans strain PDB9T NS-1 encapsulated in a calcium alginate bead system. In order to improve the mechanical stability, silica was added to the cell-embedded Ca-alginate beads. The impact of experimental conditions such as contact time, pH, and initial phenol doses was investigated. The biodegradation of phenol was examined over a wide range of phenol, and the results showed that more than 99.6% degradation was achieved at an initial phenol dose of 1000 mg/L in 70 h at 30 °C. Among the various sorption isotherm tested, the Freundlich isotherm was the best fitted to the experimental data. This behavior indicated a multilayer biosorption process and was controlled by heterogeneous surface energy. Based on an intra-particle diffusion model, internal mass transfer or pore diffusion predominated over exterior mass transfer in controlling the entire phenol biosorption process. The biosorption of phenol onto the cell encapsulated in the Ca-alginate bead follows pseudo-first-order kinetics with a superior phenol biosorption capacity of 155 mg/g of Ca-alginate. Further stability study revealed that the bead could be recycled successfully without any substantial decline in phenol degradation efficiency, indicating that the immobilized microbe possesses exceptional operating stability.

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Availability of data and materials

All data generated or analyzed during this study are included in the manuscript. The raw datasets or readings generated during and/or analyzed during the current study but not included in the manuscript are available from the corresponding author upon reasonable request

Funding

The authors acknowledge the financial support received from the Department of Biotechnology, Ministry of Science & Technology, Government of India (SAN No. 102/IFD/SAN/3612/2016-2017), BT/PR15242/BCE/8/1144/2015, for carrying out this research work.

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Authors and Affiliations

  1. Department of Chemistry, Environmental Science Program, (ITER), Siksha ‘O’ Anusandhan (Deemed to be University), Bhubaneswar, Odisha, -751030, India

    Ankita Priyadarshini, Soumya Mishra & Naresh Kumar Sahoo

  2. Centre for Biotechnology, Siksha ‘O’Anusandhan (Deemed to be University), Bhubaneswar, India

    Sangeeta Raut

  3. School of Environment & Natural Resources, Doon University, Dehradun, Uttarakhand, 248012, India

    Achlesh Daverey

  4. Faculty of Chemical Sciences (AcSIR), Department of Hydro and Electrometallurgy, Institute of Minerals and Materials Technology, Bhubaneswar, 751 013, India

    Bankim Chandra Tripathy

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  1. Ankita Priyadarshini
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Contributions

Ankita Priyadarshini: investigation, methodology, formal analysis, validation, writing – original draft, writing – review and editing

Soumya Mishra: investigation, formal analysis, writing – review and editing

Naresh Kumar Sahoo: conceptualization, visualization, methodology, investigation, supervision, resources, writing – original draft, formal analysis, writing – review and editing, funding acquisition

Sangeeta Raut: conceptualization, visualization, data curation, formal analysis, review and editing

Achlesh Daverey: conceptualization, visualization, data curation, formal analysis, review and editing

Bankim Chandra Tripathy: conceptualization, supervision, visualization, data curation, formal analysis, review and editing

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Correspondence to Naresh Kumar Sahoo.

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Priyadarshini, A., Mishra, S., Sahoo, N.K. et al. Biodegradation of Phenol Using the Indigenous Rhodococcus pyridinivorans Strain PDB9T NS-1 Immobilized in Calcium Alginate Beads. Appl Biochem Biotechnol (2023). https://doi.org/10.1007/s12010-023-04508-8

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