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Increased density of fluorescent adrenergic fibers around the middle cerebral arteries of stroke-prone spontaneously hypertensive rats

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Virchows Archiv B

Summary

The distribution of fluorescent adrenergic nerve fibers in the proximal portion (horizontal segment, Hs) and the three distal portions (major branches) of the middle cerebral arteries (MCA) was examined in stroke-prone spontaneously hypertensive rats (SHRSP) aged 10, 30, 60, 90, and 180 days, by the glyoxylic acid method. The results were compared with those in agematched normotensive Wistar Kyoto (WKY) rats. While the distribution pattern of fluorescent nerve fibers in the proximal portion of WKY rats changed from a straight linear arrangement at 10 and 30 days of age to a network-like arrangement after 60 days, those from SHRSP showed a constant meshwork pattern throughout the entire examination period. In the distal portions of the MCA of both SHRSP and WKY rats at all ages examined, fluorescent nerve fibers formed a coarse network. The distribution densities of adrenergic nerve fibers in the proximal and distal portions of the MCA of SHRSP were significantly higher (P<0.01 and 0.05) than those of WKY rats at all ages examined, except in the proximal portion at 90 and 180 days of age. The difference in nerve fiber density between SHRSP and WKY rats reached a peak at 30 days of age in both proximal and distal portions, and then gradually decreased with age. The present study suggests that sympathetic hyperinnervation is an important factor in the development of hypertension, and is involved in its maintenance in SHRSP.

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References

  • Abel PW, Hermsmeyer K (1981) Sympathetic cross-innervation of SHR and genetic controls suggests a trophic influence on vascular muscle membranes. Circ Res 49:1311–1318

    PubMed  CAS  Google Scholar 

  • Alho H, Partanen M, Koistinaho J, Vaalasti A, Hervonen A (1984) Histochemically demonstrable catecholamines in sympathetic ganglia and carotid body of spontaneously hypertensive and normotensive rats. Histochemistry 80:457–462

    Article  PubMed  CAS  Google Scholar 

  • Bevan RD (1984) Trophic effects of peripheral adrenergic nerves on vascular structure. Hypertension (suppl 3): 6:19–26

    Google Scholar 

  • Bevan RD, Purdy RE, Su C, Bevan JA (1975) Evidence for an increase in adrenergic nerve function in blood vessels from experimental hypertensive rabbits. Circ Rec 37:503–508

    CAS  Google Scholar 

  • Burnstock G, Griffith SG, Sneddon P (1984) Autonomic nerves in the precapillary vessel wall. J Cardiovasc Pharmacol (Suppl 2) 6:344–353

    Google Scholar 

  • Cassis LA, Stitzel RE, Head RJ (1985) Hypernoradrenergic innervation of the caudal artery of the spontaneously hypertensive rat: An influence upon neuroeffector mechanisms. Am Soc Pharmacol Exp Ther 234:792–803

    CAS  Google Scholar 

  • Chaldakov GN, Nara Y, Horie R, Yamori Y (1989) A new view of the arterial smooth muscle cells and autonomic nerve plexus by scanning electron microscopy in spontaneously hypertensive rats. Exp Pathol 36:181–184

    PubMed  CAS  Google Scholar 

  • Dhital KK, Gerli R, Lincoln J, Milner P, Tanganelli P, Weber G, Fruschelli C, Burnstock G (1988) Increased density of perivascular nerves to the major cerebral vessels of the spontaneously hypertensive rat: differential changes in noradrenaline and neuropeptide Y during development. Brain Res 444:33–45

    Article  PubMed  CAS  Google Scholar 

  • Eccleston-Joyner CA, Gray SD (1988) Arterial hypertrophy in the fetal and neonatal sponatenously hypertensive rat. Hypertension 12:513–518

    PubMed  CAS  Google Scholar 

  • Folkow B, Hallback M, Lundgren Y, Weiss L (1972) The effects of immunosympathectomy on blood pressure and vascular reactivity in normal and spontaneously hypertensive rats. Acta Physiol Scand 84:512–523

    PubMed  CAS  Google Scholar 

  • Fujiwara T, Kondo M, Tabei R (1990) Morphological changes in cerebral vascular smooth muscle cells in stroke-prone spontaneously hypertensive rats (SHRSP). Virchows Arch B 58:377–382

    Article  PubMed  CAS  Google Scholar 

  • Furness JB, Costa M (1975) The use of glyoxylic acid for the fluorescence histochemical demonstration of peripheral stores of noradrenaline and 5-hydroxytryptamine in whole mounts. Histochemistry 41:335–352

    Article  PubMed  CAS  Google Scholar 

  • Galloway MP, Westfall TC (1982) The release of endogenous norepinephrine from the coccygeal artery of spontaneously hypertensive and Wistar-Kyoto rats. Circ Res 51:225–232

    PubMed  CAS  Google Scholar 

  • Haebara H, Ichijima K, Motoyoshi T, Okamoto K (1968) Fluorescence microscopical studies on noradrenaline in the peripheral blood vessels of spontaneously hypertensive rats. Jpn Circ J 32:1391–1400

    PubMed  CAS  Google Scholar 

  • Hamada M, Nishio I, Baba A, Fukuda K, Takeda J, Ura M, Hano T, Kuchii M, Masuyama Y (1990) Enhanced DNA synthesis of cultured vascular smooth muscle cells from spontaneously hypertensive rats. Atherosclerosis 81:191–198

    Article  PubMed  CAS  Google Scholar 

  • Hano T, Rho J (1989) Norepinephrine overflow in perfused mesenteric arteries of spontaneously hypertensive rats. Hypertension 14:44–53

    PubMed  CAS  Google Scholar 

  • Hart MN, Heistad DD, Brody MJ (1980) Effect of chronic hypertension and sympathetic denervation on wall/lumen ratio of cerebral vessels. Hypertension 2:419–423

    PubMed  CAS  Google Scholar 

  • Ichijima K (1969) Morphological studies on the peripheral small arteries of spontaneously hypertensive rats. Jpn Circ J 33:785–812

    PubMed  CAS  Google Scholar 

  • Kanbe T, Nara Y, Tagami M, Yamori Y (1983) Studies of hypertension-induced vascular hypertrophy in cultured smooth muscle cells from spontaneously hypertensive rats. Hypertension 5:887–892

    PubMed  CAS  Google Scholar 

  • Karr-Dullien V, Bloomquist EI, Beringer T, El-Bermani Al-W (1981) Arterial morphometry in neonatal and infant spontaneously hypertensive rats. Blood Vessels 18:253–262

    PubMed  CAS  Google Scholar 

  • Kawai Y, Ohhashi T (1986) Histochemical studies of the adrenergic innervation of canine cerebral arteries. J Autun Nerv Syst 15:103–108

    Article  CAS  Google Scholar 

  • Kawamura K, Ando K, Takebayashi S (1989) Perivascular innervation of the mesenteric artery in spontaneously hypertensive rats. Hypertension 14:660–665

    PubMed  CAS  Google Scholar 

  • Kobayashi S, Tsukahara S, Sugita K, Nagata T (1981) Adrenergic and cholinergic innervation of rat cerebral arteries. Histochemistry 70:129–138

    Article  PubMed  CAS  Google Scholar 

  • Kondo M (1986) Autoradiographic study of3H-lysine uptake by superior cervical and stellate ganglia in prehypertensive spontaneously hypertensive rats. Virchows Arch B 52:299–304

    PubMed  CAS  Google Scholar 

  • Kondo M (1987) Autoradiographic study of3H-DOPA uptake by superior cervical and stellate ganglia of spontaneously hypertensive rats during the prehypertensive stage. Virchows Arch B 54:190–193

    PubMed  CAS  Google Scholar 

  • Kondo M, Terada M, Shimizu D, Fujiwara T, Tabei R (1990) Morphometric study of the superior cervical and stellate ganglia of spontaneously hypertensive rats during the prehypertensive stage. Virchows Arch B 58:371–376

    PubMed  CAS  Google Scholar 

  • Lais LT, Brody MJ (1978) Vasoconstrictor hyperresponsiveness: an early pathogenetic mechanism in the spontaneously hypertensive rat. Eur J Pharmacol 47:177–189

    Article  PubMed  CAS  Google Scholar 

  • Lee RMKW (1985) Vascular changes at the prehypertensive phase in the mesenteric arteries from spontaneously hypertensive rats. Blood Vessels 22:105–126

    PubMed  CAS  Google Scholar 

  • Lee RMKW, Forrest JB, Garfield RE, Daniel EE (1983) Ultrastructural changes in mesenteric arteries from spontaneously hypertensive rats. Blood Vessels 20:72–91

    PubMed  CAS  Google Scholar 

  • Lee RMKW, Smeda JS (1985) Primary versus secondary structural changes of the blood vessels in hypertension. Can J Physiol Pharmacol 63:392–401

    PubMed  CAS  Google Scholar 

  • Lee RMKW, Triggle CR, Cheung DWT, Coughlin MD (1987) Structural and functional consequence of neonatal sympathectomy on the blood vessels of spontaneously hypertensive rats. Hypertension 10:328–338

    PubMed  CAS  Google Scholar 

  • Lee TJ-F, Saito A (1984) Altered cerebral vessel innervation in the spontaneously hypertensive rat. Circ Res 55:392–403

    PubMed  CAS  Google Scholar 

  • Lee TJ-F, Saito A (1986) Altered cerebral vessel innervation in spontaneously hypertensive and renal hypertensive rats. J Hypertension 4 (suppl 3):s201-s203

    CAS  Google Scholar 

  • Longhurst PA, Stitzel RE, Head RJ (1986) Perfusion of the intact and partially isolated rat mesenteric vascular bed: Application to vessels from hypertensive and normotensive rats. Blood Vessels 23:288–296

    PubMed  CAS  Google Scholar 

  • Masuyama Y, Tsuda K, Kusuyama Y, Hano T, Kuchii M, Nishio I (1984) Neurotransmitter release, vascular responsiveness and their calcium-mediated regulation in perfused mesenteric preparation of spontaneously hypertensive rats and DOCA-salt hypertension. J Hypertension 2 (suppl 3):99–102

    CAS  Google Scholar 

  • Miller BG, Connors BA, Bohlen G, Evan AP (1987) Cell and wall morphology of intestinal arterioles from 4- to 6- and 17-to 19-week-old Wistar-Kyoto and spontaneously hypertensive rats. Hypertension 9:59–68

    PubMed  CAS  Google Scholar 

  • Mione MC, Dhital KK, Amenta F, Burnstock G (1988b) An increase in the expression of neuropeptidergic vasodilator, but not vasoconstrictor, cerebrovascular nerves in aging rats. Brain Res 460:103–113

    Article  PubMed  CAS  Google Scholar 

  • Mione MC, Erdo SL, Kiss B, Ricci A, Amenta F (1988a) Agerelated changes of noradrenergic innervation of rat splanchnic blood vessels: a histofluorescence and neurochemical study. J Autun Nerv Syst 25:27–33

    Article  CAS  Google Scholar 

  • Mulvany MJ, Baandrup U, Gundersen HJG (1985) Evidence for hyperplasia in mesenteric resistance vessels of spontaneously hypertensive rats using a three-dimensional disector. Circ Res 57:794–800

    PubMed  CAS  Google Scholar 

  • Nordborg C, Fredriksson K, Johansson BB (1985) The morphometry of consecutive segments in cerebral arteries of normotensive and spontaneously hypertensive rats. Stroke 16:313–320

    PubMed  CAS  Google Scholar 

  • Owens GK, Schwartz SM (1982) Alterations in vascular smooth muscle mass in the spontaneously hypertensive rats; Role of cellular hypertrophy, hyperploidy, and hyperplasia. Circ Rec 51:280–289

    CAS  Google Scholar 

  • Schon F, Allen JM, Yeats JC, Allen YS, Ballesta J, Polak JM, Kelly JS, Bloom SR (1985) Neuropeptide Y innervation of the rodent pineal gland and cerebral blood vessels. Neurosci Lett 57:65–71

    Article  PubMed  CAS  Google Scholar 

  • Scott TM, Pang SC (1983) The correlation between the development of sympathetic innervation and the development of medial hypertrophy in jejunal arteries in normotensive and spontaneously hypertensive rats. J Autun Nerv Syst 8:25–32

    Article  CAS  Google Scholar 

  • Sinaiko AR, Cooper MJ, Mirkin BL (1980) Effect of neonatal sympathectomy with 6-hydroxydopamine on the reactivity of the renin-angiotensin system in the spontaneously hypertensive rat. Clin Sci 59:123–129

    PubMed  CAS  Google Scholar 

  • Tsuda K, Kuchii M, Nishio I, Masuyama Y (1986) Effects of epinephrine and dopamine on norepinephrine release from the sympathetic nerve endings in hypertension. J Hypertension 4 (suppl 5):s45-s48

    CAS  Google Scholar 

  • Tsuda K, Shima H, Ura M, Takeda J, Kimura K, Nishio I, Masuyama Y (1988) Protein kinase C-dependent and calmodulin-dependent regulation of neurotransmitter release and vascular responsiveness in spontaneously hypertensive rats. J Hypertension 6 (suppl 4):s565–567

    Google Scholar 

  • Yang H, Morton W, Lee RMKW, Kajetanowicz A, Forrest JB (1989) Autoradiographic study of smooth muscle cell proliferation in spontaneously hypertensive rats. Clin Sci 76:475–478

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Pathology, Ehime University School of Medicine, Shitsukawa, Shigenobu, Onsen-gun, 791-02, Ehime, Japan

    Mari Kondo, Tatsuhiko Miyazaki & Ryo Tabei

  2. Laboratory Animal Center, Ehime University School of Medicine, Shitsukawa, Shigenobu, Onsen-gun, 791-02, Ehime, Japan

    Takashi Fujiwara

  3. Ehime College of Health Science, Takouda, Tobe, Iyo-gun, 791-21, Ehime, Japan

    Akemi Yano

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  1. Mari Kondo
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  2. Tatsuhiko Miyazaki
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  3. Takashi Fujiwara
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  4. Akemi Yano
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  5. Ryo Tabei
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Kondo, M., Miyazaki, T., Fujiwara, T. et al. Increased density of fluorescent adrenergic fibers around the middle cerebral arteries of stroke-prone spontaneously hypertensive rats. Virchows Archiv B Cell Pathol 61, 117–122 (1992). https://doi.org/10.1007/BF02890413

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  • DOI: https://doi.org/10.1007/BF02890413

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