Abstract
Two keratin-like proteins of 64 and 55 ku were purified from suspension cells ofDaucus carota L., and their partial amino acid sequences were determined. The homological analysis showed that the sequence from the 64 ku protein was highly homological to β-glucosidase, and that from the 55 ku protein had no significant homologue in GenBank. Using conservative sequence of animal IF proteins as primer, we cloned a cDNA fragment fromDaucus carota L. Southern blot and Northern blot results indicated that this cDNA fragment was a single copy gene and expressed both in suspension cells and leaves. Homological analysis revealed that it had moderate homology to a variety of α-helical proteins. Our results might shed more light on molecular characterization of IF existence in higher plant.
Similar content being viewed by others
References
Fuchs, E., Weber, K., Intermediate filament: structure function, and disease, Annu. Rev. Biochem., 1994, 63: 345.
Traub, P., Shoeman, R.L., Intermediate filament proteins-cytoskeletal elements with gene regulatory function? Internal Review of Cytology, 1994, 15: 1.
Powell, A., Peace, G., Slabas, A. et al., The detergent resistant cytoskeleton of higher plant protoplasts contains nucleus associated fibrillar bundles in addition to microtubules, J. Cell. Sci., 1982, 56: 319.
Su, F., Gu, W., Zhai, Z. H., The keratin intermediate filament-like system in the plant cells, J. Chinese EM (in Chinese), 1988, 3:3.
Yang, C., Xing, L., Zhai, Z.H., Intermediate filaments in higher plant cells and their assembly in a cell free system, Protoplasma, 1992, 171: 44.
Wang, J., Yang, C., Zhai, Z., The nuclear Lamina in male generative cells ofGinko biloba, Sex Plant Reprod., 1996, 9: 238.
Chen, D., Zhao, Y., Zhao, D. et al., Studies on intermediate filament inAdlantum philippense L. and comparison of keratin-like proteins in some higher plants, Acta Botanica Sinica (in Chinese), 1998, 40(9): 790.
Zhao, D., Zhao, Y., Chen, D. et al., Homological analysis of intermediate filament proteins, Acta Scientiarum Naturalium Universitatis Pekinensis (in Chinese), 1998, 34:770.
Li, X., Cai, S., Zhai, Z., Proof of existence of IF-like system in DinoflagellatesCrypthecodinium cohnii (in Chinese), Chin. Sci. Bull., 1992, 37:2178.
Fan, B., Lin, Y., Zhai, Z., Existense of cage-like structure consisting of intermediate filament fibril in basal bodies ofChlamydomonas sp., Chin. Sci. Bull. (in Chinese), 1994, 27:131.
Berger, S., Shoeman, R.L., Traub, P., Detection of dense intra- and perinuclear 10 nm filament systems by whole mount and embedment-free electron microscopy in several species of the green algal order Dasycladales, Protoplasma, 1996, 190: 204.
Numata, O., Watanabe, Y.,In vitro assembly and disassembly of 14-nm filament fromTetrahymena pyriformis, The protein component of 14-nm filament is a 49000-Dalton protein, J. Biochem., 1982, 91: 1563.
Numata, O., Takemasa, T., Takagi, I. et al., Tetrahymena 14-nm filament-forming protein has citrate synthase activity, Biochem. Biophs. Res. Comm., 1991, 174: 1028.
Piatigorsky, J., Wistow, G.J., Enzymel/crystalline: gene sharing as an evolutionary strategy, Cell, 1989, 57: 197.
Hughes, M.A., Brown, K., Pancoro, A., A molecular and biochemical analysis of the structure of the cyanogenic β-glucosidase (linamarase) from cassava (Manihot esculenta Cranz), Arch. Biochem. Biophs., 1992, 295(2): 273.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Zhao, D., Chen, D., Yang, C. et al. Sequence analysis of keratin-like proteins and cloning of intermediate filament-like cDNA from higher plant cells. Sci. China Ser. C.-Life Sci. 43, 265–271 (2000). https://doi.org/10.1007/BF02879285
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02879285