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The establishment of cell suspension cultures ofGladiolus that regenerate plants

  • Plant Cellular and Developmental Biology
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Summary

Inflorencence stalks from greenhouse-grownGladiolus plants of the cultivars ‘Blue Isle’ and ‘Hunting Song’ cultured on a Murashige and Skoog basal salts medium supplemented with 53.6 μM 1-napthaleneacetic acid formed a compact, not friable type of callus that regenerated plantlets. Cormel slices and intact plantlets of three cultivars (‘Peter Pears’, ‘Rosa Supreme’, ‘Jenny Lee’) propagated through tissue culture formed a friable type of callus when cultured on Murashige and Skoog basal salts medium supplemented with 2,4-dichlorophenoxyacetic acid. This friable callus readily formed a cell suspension when the callus was placed in a liquid medium. Plants were regenerated from two-month-old suspension cell cultures of the commercial cultivar ‘Peter Pears’ after the suspension cells had been cultured on solid medium.

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Authors and Affiliations

  1. United States Department of Agriculture, Florist and Nursery Crops Laboratory, Beltsville Agricultural Research Center, 20705, Beltsville, Maryland

    Kathryn Kamo, Janet Chen & Roger Lawson

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  1. Kathryn Kamo
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  2. Janet Chen
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  3. Roger Lawson
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Kamo, K., Chen, J. & Lawson, R. The establishment of cell suspension cultures ofGladiolus that regenerate plants. In Vitro Cell Dev Biol 26, 425–430 (1990). https://doi.org/10.1007/BF02623835

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  • DOI: https://doi.org/10.1007/BF02623835

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