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Isolation and culture of cereal protoplasts

Part 2: Embryogenesis and plantlet formation from protoplasts of Pennisetum americanum

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Summary

Protoplasts isolated from embryogenic suspension cultures derived from immature embryos of pearl millet (Pennisetum americanum) gave rise to cell masses. These cell masses upon transfer to a hormone-free medium formed embryoids, which further developed into plantlets with roots and shoots.

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Literature

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Communicated by P. Maliga

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Vasil, V., Vasil, I.K. Isolation and culture of cereal protoplasts. Theoret. Appl. Genetics 56, 97–99 (1980). https://doi.org/10.1007/BF00265079

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  • DOI: https://doi.org/10.1007/BF00265079

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