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High-frequency plant regeneration from embryogenic cell suspension cultures of Gynura procumbens

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Abstract

The efficient plant regeneration system from embryogenic cell suspension cultures of Gynura procumbens (Lour.) Merr. is described. Leaf, stem and petiole explants were cultured on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) in various concentrations (0, 0.1, 0.3, 1.0 and 3.0 mg l−1). Leaf, stem and petiole explants formed pale-yellow nodular callus and off-white calluses at a frequency of 100% when cultured on MS medium supplemented with more than 1 mg l−1 of 2,4-D after 4 weeks incubation. However, only 20% of pale-yellow nodular callus derived from petiole explants developed into white embryonic structures. Upon transfer to MS basal medium without growth regulators, these white embryonic structures differentiated into somatic embryos. Embryogenic cell suspension cultures were initiated from petiole-derived pale-yellow nodular calluses. More than 73.2% of regenerated plantlets via somatic embryogenesis produced roots on MS medium supplemented with 0.1 mg l−1 α-naphthaleneacetic acid and 1 mg l−1 indole-3-butyric acid (IBA), respectively. Rooted plantlets were successfully transplanted to soil mixture of sterile vermiculite and potting soil (1:1) and grown to maturity in a growth chamber, achieving a survival rate of > 95%. The plant regeneration system from embryogenic cell suspension cultures of G. procumbens established in this study could be applied as an alternative for mass proliferation as well as molecular breeding for quality improvement of G. procumbens.

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Acknowledgements

This work was supported by a grant from the KRIBB Research Initiative Program (KGM5281711).

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Author notes

  1. Eun Yee Jie and Nornita Sham Atong contributed equally.

Authors and Affiliations

  1. Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 181 Ipsin-gil, Jeongeup-si, Jeollabuk-do, 580-185, Republic of Korea

    Eun Yee Jie, Woo Seok Ahn & Suk Weon Kim

  2. Department of Crop Science, Faculty of Agriculture, University Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Nornita Sham Atong & Saleh Kadzimin

  3. Floriculture Research Division, National Institute of Horticultural and Herbal Science, Wanju-gun, Jeollabuk-do, 55365, Republic of Korea

    Myung Suk Ahn

  4. School of Ecological and Environmental System, Kyungpook National University, Sangju-si, 742-711, Republic of Korea

    Byung Hwan Min

Authors
  1. Eun Yee Jie
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  2. Nornita Sham Atong
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  3. Woo Seok Ahn
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  4. Myung Suk Ahn
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  5. Byung Hwan Min
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  6. Saleh Kadzimin
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  7. Suk Weon Kim
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Correspondence to Suk Weon Kim.

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Jie, E.Y., Atong, N.S., Ahn, W.S. et al. High-frequency plant regeneration from embryogenic cell suspension cultures of Gynura procumbens. Plant Biotechnol Rep 13, 27–33 (2019). https://doi.org/10.1007/s11816-018-0507-6

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  • DOI: https://doi.org/10.1007/s11816-018-0507-6

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