Summary
The separation by capillary electrophoresis of five ecstasy derivatives has been evaluated by means of experimental designs. A full-factorial design and a central composite design were employed to optimize the experimental conditions for a fast separation. With a conventional capillary (62.5 cm length) analytes were separated in less than 8 min. When the capillary length was reduced the analysis time decreased substantially. Comparisons were made between different procedures (e.g. working at a constant field or at constant voltage) and finally, a fast separation of five Ecstasy derivatives was achieved in 1 min.
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References
C. Battu, P. Marquet, A. L. Fauconnet, E. Lacassie, G. Lachâtre, J. Chromatogr. Sci.,36 1 (1998).
T. Kraemer, H. H. Maurer, J. Chromatogr. A,713 163 (1998).
D. M. Northrop, B. R. McCord, J. M. Butler, J. Capillary Electrophor.1 158 (1994).
E. Sadeghipour, J. -L. Veuthey, J. Pharm. Biomed. Anal.,17 801 (1998).
C. Giroud, M. Augsburger, F. Sadeghipour, E. Varesio, J. -L. Veuthey, L. Rivier, P. Mangin, Toxicorama,9 113 (1997).
K. A. Lilley, T. E. Wheat, J. Chromatogr. B,683 67 (1996).
J. C. Hudson, M. Golin, M. Malcolm, Can. Soc. Forens. Sci. J.28 137 (1995).
V. C. Trenerry, J. Robertson, R. J. Wells, J. Chromatogr. A,708, 169 (1995).
F. Sadeghipour, E. Varesio, C. Giroud, L. Rivier, J. -L. Veuthey, Forens. Sci. Int.,86 1 (1997).
G. E. P. Box, W. G. Hunter, J. S. Hunter, Statistics for Experimenters, An Introduction to Design, Data Analysis and Model Building, Wiley, New York, 1978.
S. N. Deming andS. L. Morgan, Experimental Design: A Chemometric Approach, Elsevier, Amsterdam, 1993.
J. Goupy, Methods for Experimental Design, Principles and Applications for Physicists and Chemists, Elsevier, Amsterdam, 1993.
P. Lantéri, R. Longeray, Analusis,24 M17 (1996).
K. D. Altria, S. D. Filbey, Chromatographia,39 306 (1994).
M. Jimidar, B. Bourguignon, D. L. Massart J. Chromatogr. A740 109 (1996).
M. M. Rogan, K. D. Altria, Chromatographia,38 723 (1994).
T. S. Small, A. F. Fell, M. W. Coleman, J. C. Berridge, Chirality7 226 (1995).
E. Varesio, J. -Y. Gauvrit, R. Longeray, P. Lantéri, J. -L. Veuthey, Electrophoresis,18 931 (1997).
J. H. Knox, K. A. McCormack, Chromatographia,38 279 (1994).
K. D. Altria, Chromatographia,35 177 (1993).
K. D. Altria, J. Chromatogr.,646 245 (1993).
D. L. Massart, B. G. M. Vandeginste, L. M. C. Buydens, S. De Jong, P. J. Lewi, J. Smeyers-Verbeke, Handbook of Chemometrics and Qualimetrics: Part A, Elsevier, Amsterdam, 1997.
R. Kuhn, S. Hoffstetter-Kuhn, Capillary Electrophoresis: Principles and Practice, Springer, Berlin, 1993, p. 34.
X. Huang, W. F. Coleman, R. N. Zare, J. Chromatogr.,480 95 (1989).
J. W. Jorgenson, K. D. Lukacs, Science,222 266 (1983).
S. Fanali, S. Furlanetto, Z. Aturki, S. Pinzauti, Chromatographia48 395 (1998).
G. E. P. Box, K. B. Wilson, J. Royal Stat. Soc.,13 1 (1951).
N. R. Draper, H. Smith, Applied Regression Analysis, Wiley, New York, 1981.
A. Aumatell, A. Guttman, J. Chromatogr. A,717 229 (1995).
K. D. Altria, M. A. Kelly, B. J. Clark, Chromatographia,43 153 (1996).
K. D. Altria, J. Chromatogr.,636 125 (1993).
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Varesio, E., Gauvrit, J.Y., Longeray, R. et al. Optimization of fast CE analyses of ecstasy derivatives by use of experimental designs. Chromatographia 50, 195–201 (1999). https://doi.org/10.1007/BF02490651
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DOI: https://doi.org/10.1007/BF02490651