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Experimental Design Methodologies for the Optimization of Chiral Separations: An Overview

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Chiral Separations

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1985))

Abstract

In this chapter, the application of design of experiments (DoE) for chiral separation optimization using supercritical fluid chromatography (SFC), liquid chromatography (LC), capillary electrophoresis (CE), and capillary electrochromatography (CEC) methods is reviewed. Both screening and optimization steps are covered, including a discussion of each aspect, such as factor-, level-, and response selection. Different designs are also presented, highlighting their applications.

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Notes

  1. 1.

    Factors are variables that can be varied in an independent manner from each other, e.g., pH, temperature, concentration [9, 12].

  2. 2.

    Levels are the values of a specific factor. Therefore, temperature (a factor), for example, can be evaluated at 30, 40, and 50 °C (levels) [9, 12].

  3. 3.

    Response is the measurable output from experiments, e.g., migration time, resolution [12].

  4. 4.

    Experimental domain is defined by the ranges of the factors levels [12].

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Author information

Authors and Affiliations

  1. Pharmaceutical Chemistry Research Group, Universidade do Vale do Itajaí (UNIVALI), Itajaí, SC, Brazil

    Luiz Carlos Klein-Júnior

  2. Department of Analytical Chemistry, Applied Chemometrics and Molecular Modelling, Vrije Universiteit Brussel (VUB), Brussels, Belgium

    Debby Mangelings & Yvan Vander Heyden

Authors
  1. Luiz Carlos Klein-Júnior
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  2. Debby Mangelings
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  3. Yvan Vander Heyden
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Corresponding author

Correspondence to Yvan Vander Heyden .

Editor information

Editors and Affiliations

  1. Department of Pharmaceutical Chemistry, University of Jena, Jena, Germany

    Gerhard K. E. Scriba

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Klein-Júnior, L.C., Mangelings, D., Vander Heyden, Y. (2019). Experimental Design Methodologies for the Optimization of Chiral Separations: An Overview. In: Scriba, G.K.E. (eds) Chiral Separations. Methods in Molecular Biology, vol 1985. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9438-0_27

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  • DOI: https://doi.org/10.1007/978-1-4939-9438-0_27

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9437-3

  • Online ISBN: 978-1-4939-9438-0

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