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Plackett-Burman experimental design in chiral analysis using capillary electrophoresis

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Summary

Plackett-Burman experimental design has been studied in the chiral separation of clenbuterol using capillary electrophoresis (CE). This saturated fractional design approach was used to simultaneously investigate the variables pH, cyclodextrin concentration, electrolyte ionic strength, methanol concentration, and injection time, each at three levels. Percentage main effects of each variable on resolution, retention time and peak efficiency were calculated. Ionic strength, pH and β-cyclodextrin concentration were found to exert the greatest effect on resolution, pH on retention time and pH and ionic strength on peak efficiency. Results are in agreement with those from a previous univariate study. It is concluded that Plackett-Burman experimental design offers of rapid means for testing robustness of chiral CE methods or for the simultaneous screening of experimental variables.

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References

  1. S. G. Allenmark, Chromatographic Enantioseparation: Methods and Applications, Ellis Horwood, Chichester, 2nd Ed., 1991.

    Google Scholar 

  2. R. A. Wallingford, A. G. Ewing, Adv. Chromatogr.29, 1 (1989).

    PubMed  Google Scholar 

  3. W. G. Kuhr, Anal. Chem.62, 403R (1990).

    Google Scholar 

  4. E. Gassmann, L. E. Kuo, R. N. Zare, Science230, 813 (1985).

    Google Scholar 

  5. S. Terabe, M. Shibata, Y. Miyashita, J. Chromatogr.480, 403 (1989).

    Article  Google Scholar 

  6. S. Fanali, J. Chromatogr.474, 441 (1989).

    Article  Google Scholar 

  7. R. Kuhr, F. Stoecklin, F. Erni, Chromatographia33, 32 (1992).

    Google Scholar 

  8. M. M. Rogan, D. M. Goodall, K. D. Altria, Chirality,6, 25 (1994).

    Article  Google Scholar 

  9. M. M. Rogan, K. D. Altria, 2nd Int. Symp. on Chiral Discrimination, Rome, 1991.

  10. M. M. Rogan, C. Drake, D. M. Goodall, K. D. Altria, Anal. Biochem.208, 343 (1993).

    Article  PubMed  Google Scholar 

  11. K. D. Altria, D. M. Goodall, M. M. Rogan, Chromatographia34, 19 (1992).

    Google Scholar 

  12. J. C. Berridge, Analyst112, 385 (1987).

    Article  Google Scholar 

  13. S. N. Deming, S. L. Morgan, Anal. Chim. Acta.150, 183 (1983).

    Article  Google Scholar 

  14. R. C. Plackett, J. P. Burman, Biometrica23, 305 (1946).

    Google Scholar 

  15. R. A. Stowe, R. P. Mayer, Ind. Eng. Chem.58, 36 (1966).

    Article  Google Scholar 

  16. K. Jones, Chromatographia25, 36 (1988).

    Google Scholar 

  17. M. Mulholland, J. Waterhouse, J. Chromatogr.395, 539 (1987).

    Article  Google Scholar 

  18. J. Vindevogel, P. Sandra, Anal. Chem.63, 1530 (1991).

    Article  Google Scholar 

  19. G. E. P. Box, W. G. Hunter, J. S. Hunter, Statistics for Experimenters, An Introduction to Design, Data Analysis and Model Building, Wiley, New York, 1978, Part III.

    Google Scholar 

  20. D. Burgi, J. Microcolumn Sep.3, 199 (1991).

    Article  Google Scholar 

  21. S. A. Wren, R. C. Rowe, J. Chromatogr.603, 235 (1992).

    Article  Google Scholar 

  22. S. G. Penn, D. M. Goodall, J. S. Loran, J. Chromatogr.636, 149 (1993).

    Article  PubMed  Google Scholar 

  23. S. G. Penn, E. T. Bergstrom, D. M. Goodall, J. S. Loran, Anal. Chem., submitted.

  24. S. Fujiwara, S. Honda, Anal. Chem.59, 487 (1987).

    Article  Google Scholar 

  25. T. Tsuda, K. Nomura, G. Nakagawa, J. Chromatogr.248, 241 (1982).

    Article  Google Scholar 

  26. Y. Y. Rawjee, R. L. Williams, Gy Vigh, J. Chromatogr.635, 291 (1993).

    Article  Google Scholar 

  27. S. Fanali J. Chromatogr.545, 437 (1991).

    Article  Google Scholar 

  28. D. W. Armstrong, International Symposium on Chiral Discrimination, Tübingen 1992.

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Authors and Affiliations

  1. Respiratory Analysis Department, Glaxo Group Research, Park Road, SG12 ODP, Ware, Hertfordshire, UK

    M. M. Rogan

  2. Analytical Evaluation Group, Glaxo Group Research, Park Road, SG12 ODP, Ware, Hertfordshire, UK

    K. D. Altria

  3. Department of Chemistry, University of York, Y01 5DD, Heslington, York, UK

    D. M. Goodall

Authors
  1. M. M. Rogan
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  2. K. D. Altria
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  3. D. M. Goodall
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Rogan, M.M., Altria, K.D. & Goodall, D.M. Plackett-Burman experimental design in chiral analysis using capillary electrophoresis. Chromatographia 38, 723–729 (1994). https://doi.org/10.1007/BF02269627

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  • DOI: https://doi.org/10.1007/BF02269627

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