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Experimental design methodologies to optimize the CE separation of epinephrine enantiomers

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Summary

A capillary electrophoretic method using a chiral selector was optimized by experimental design for the enantioresolution of epinephrine enantiomers. Two β-cyclodextrins derivatives, namely heptakis-2,6-di-O-methyl-β-cyclodextrin and carboxy-methyl-β-cyclodextrin, respectively neutral and charged, were used as chiral selectors employing an uncoated capillary. By using a statistical experimental design in which all factors are varied at the same time, it was possible to optimize the method with regard to the resolution between peaks and the two migration times. A fractional factorial design and a central composite design were used. A compromise between conflicting goals, such as maximization of resolution and minimization of analysis time, was found by means of a desirability function D. Balancing these goals against each other, the most acceptable solution to the problem was found and the optimized method gave a fast separation with complete resolution between the adrenaline enantiomers. The response surfaces obtained confirmed the robustness of the method.

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References

  1. I. R. Innes, M. Nickersen inL. S. Goodman, A. Gilman (Eds.), The Pharmacological Basis of Therapeutics, MacMillan Publishing Co., Inc, New York, 1970, p. 477.

    Google Scholar 

  2. S. Fabro, R. L. Smith, T. Williams, Nature215, 296 (1967).

    Article  CAS  Google Scholar 

  3. J. Debowski, D. Sybilska andJ. Jurczak, J. Chromatogr.282, 83 (1983).

    Article  CAS  Google Scholar 

  4. G. Blaschke, J. Liq. Chromatogr.9, 341 (1986).

    CAS  Google Scholar 

  5. L. Dou, J. N. Zeng, D. D. Gerochi, M. P. Duda, H. H. Stuting, J. Chromatogr. A679, 367 (1994).

    Article  CAS  Google Scholar 

  6. D. W. Armstrong, J. R. J. Faulkner, S. M. Han, J. Chromatogr.452, 323 (1988).

    Article  CAS  Google Scholar 

  7. N. H. Singh, F. N. Pasutto, R. T. Coutts, F. Jamali, J. Chromatogr.378, 125 (1986).

    CAS  Google Scholar 

  8. F. Kobor, G. Schomburg, J. High Resolut. Chromatogr.16, 693 (1993).

    Article  CAS  Google Scholar 

  9. A. Guttman, A. Paulus, A. S. Cohen, N. Grinberg, B. L. Karger, J. Chromatogr.448, 41 (1988).

    Article  CAS  Google Scholar 

  10. S. Terabe, H. Ozaki, K. Otsuka, T. Ando, J. Chromatogr.332, 211 (1985).

    Article  CAS  Google Scholar 

  11. S. Fanali, J. Chromatogr.474, 441 (1989).

    Article  CAS  Google Scholar 

  12. K. D. Altria, D. M. Goodall, M. M. Rogan, Chromatographia34, 19 (1992).

    Article  CAS  Google Scholar 

  13. T. Schmitt, H. Engelhardt, Chromatographia37, 475 (1993).

    Article  CAS  Google Scholar 

  14. B. Chankvetadze, G. Endresz, G. Blaschke, Electrophoresis15, 804 (1994).

    Article  CAS  Google Scholar 

  15. S. Fanali, J. Chromatogr. A735, 77 (1996).

    Article  CAS  Google Scholar 

  16. S. Terabe, K. Otsuka, H. Nishi, J. Chromatogr. A666, 295 (1994).

    Article  CAS  Google Scholar 

  17. R. Vespalec, P. Bocek, Electrophoresis18, 843 (1997).

    Article  CAS  Google Scholar 

  18. S. Fanali, J. Chromatogr.545, 437 (1991).

    Article  Google Scholar 

  19. W. Schutzner, S. Fanali, Electrophoresis13, 687 (1992).

    Article  CAS  Google Scholar 

  20. J. L. Goupy, Methods for Experimental Design, Elsevier, Amsterdam, 1993.

    Google Scholar 

  21. K. D. Altria, B. J. Clark, S. D. Filbey, M. A. Kelly, D. R. Rudd, Electrophoresis16, 2143 (1995).

    Article  CAS  Google Scholar 

  22. M. Thoesteinsdottir, D. Westerlund, G. Andersson, P. Kaufmann, Chromatographia46, 545 (1997).

    Google Scholar 

  23. M. Jimidar, P. F. De Aguiar, S. Pintelon, D. L. Massart, J. Pharm. Biomed. Anal.15, 709 (1997).

    Article  CAS  Google Scholar 

  24. E. Varesio, J. Y. Gauvrit, R. Longeray, P. Lanteri, J.-L. Veuthey, Electrophoresis18, 931 (1997).

    Article  CAS  Google Scholar 

  25. K. D. Altria, D. M. Goodall, M. M. Rogan, Chromatographia34, 19 (1992).

    Article  CAS  Google Scholar 

  26. M. M. Rogan, K. D. Altria, D. M. Goodall, Chromatographia34, 166 (1994).

    Google Scholar 

  27. J. Vindevogel, P. Sandra, Anal. Chem.63, 1530 (1991).

    Article  CAS  Google Scholar 

  28. S. Furlanetto, S. Pinzauti, P. Gratteri, E. La Porta, G. Calzeroni, J. Pharm. Biomed. Anal.15, 1585 (1997).

    Article  CAS  Google Scholar 

  29. N. Kettaneh-Wold, J. Pharm. Biomed. Anal.9, 605 (1991).

    Article  CAS  Google Scholar 

  30. B. Bourguignon, D. L. Massart, J. Chromatogr.586, 11 (1991).

    Article  CAS  Google Scholar 

  31. A. C. Atkinson, A. N. Donew, Optimum Experimental Designs, Oxford Science Pubblications, Oxford, 1992.

    Google Scholar 

  32. R. Carlson, Design and optimization in organic synthesis, Elsevier, Amsterdam, 1992.

    Google Scholar 

  33. Y. Vander Heyden, D. L. Massart in M. M. W. B. Hendriks, J. H. de Boer, A. K. Smilde. Robustness of analytical chemical methods and pharmaceutical technological products, Elsevier, Amsterdam, 1996, pp. 96–103.

    Google Scholar 

  34. S. N. Deming, S. L. Morgan (Eds.), Experimental Design: A Chemometric Approach, Elsevier, Amsterdam, 1993.

    Google Scholar 

  35. D. Mathieu, R. Phan-Tan-Luu, M. Sergent, in Criblage et etude des facteurs, LPRAI, Marseille, 1996.

    Google Scholar 

  36. E. Morgan (Ed.), Chemometrics: Experimental Design, John Wiley & Sons, Chichester, 1991, pp. 238–245.

    Google Scholar 

  37. S. Pinzauti, P. Gratteri, S. Furlanetto, P. Mura, E. Dreassi, R. Phan-Tan-Luu, J. Pharm. Biomed. Anal.14, 881 (1996).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. C.N.R. Institute of Chromatography, 00016 Monterotondo Scalo, P.O. Box 10, Rome, Italy

    S. Fanali & Z. Aturki

  2. Department of Pharmaceutical Sciences, University of Florence, Via G. Capponi 9, 50121 Florence, Italy

    S. Furlanetto & S. Pinzauti

Authors
  1. S. Fanali
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  2. S. Furlanetto
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  3. Z. Aturki
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  4. S. Pinzauti
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Fanali, S., Furlanetto, S., Aturki, Z. et al. Experimental design methodologies to optimize the CE separation of epinephrine enantiomers. Chromatographia 48, 395–401 (1998). https://doi.org/10.1007/BF02467710

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  • DOI: https://doi.org/10.1007/BF02467710

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