Abstract
Physiological saline with or without substance P (50 ng/ml) was infused into the humeral artery in 6 healthy males. Indices of fibrinolytic activity (whole blood diluted lysis time, euglobulin lysis time, lysis areas in non-heated fibrin plates produced by plasma or euglobulin precipitate, plasminogen plasma levels, α2-macroglobulin, Cl-inhibitor, and α2-antiplasmin) were evaluated in the homolateral antecubital vein before and after 5 min of substance P or saline infusion. After substance P the fibrinolytic activity increased, as can be seen from the shortening of lysis times (p<0.01) and enlargement of the lysis areas (p<0.01). A reduction of plasminogen plasma levels (p<0.01), associated with a decrease in α2-anitplasmin (p<0.01), was also found. Alpha2-macroglobulin and Cl-inhibitor were instead unaltered by the peptide. The saline infusion, on the other hand, was unable to modify any of the examined indices. We concluded that exogenous substance P given intra-arterially increases fibrinolytic activity in locally-sampled venous blood through a mechanism which remains to be elucidated.
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Wasantapruek, S., Poolsuppasit, S., and Pibolnukarintr, O., New Engl. J. Med.290 (1974) 259.
Visudhipan, S., Poolsuppasit, S., and Pibolnukarintr, O., Am. J. clin. Nutr.35 (1982) 1452.
Holzer, P., Neuroscience24 (1988) 739.
Maggi, C. A., and Meli, A., Gen. Pharmac.19 (1988) 1.
Regoli, D., Drapeau, G., Dion, S., and D'Orleans-Juste, P., Pharmacology38 (1989) 1.
Sicuteri, F., Michelacci, S., Lombardi, V., and Fanciullacci, M., Archs int. Pharmacodyn. Thér.151 (1964) 38.
Fearnley, G. R., Balforth, G., and Fearnley, E., Clin. Sci.16 (1957) 645.
Kluft, C., and Brakman, P., in: Progress in Chemical Fibrinolysis and Thrombolysis, vol. I, p. 375. Eds J. F. Davidson, M. M. Samama and P. C. Desnoyers. Raven Press, New York 1975.
Nilsson, I. M., Medner, U., and Pandolfi, M., in: Fibrinolytics and Antifibrinolytics, p. 107. Ed. F. Markwardt. Springer-Verlag, Berlin 1978.
Mancini, G., Carbonara, A. O., and Heremans, J. F., Immunochemistry2 (1965) 235.
Gallimore, M. J., Amundsen, E., Aasen, A. O., Braaten, M. L., Lyngaas, K., and Svendsen, L., Thromb. Res.14 (1979) 51.
Matsas, R., Fulcher, I. S., Kenny, A. J., and Turner, A. J., Proc. natl Acad. Sci. USA80 (1983) 3111.
Sekizawa, K., Tamaoki, J., Graf, P. D., Basbaum, C. B., Barson, D. B., and Nadel, J. A., J. Pharmac. exp. Ther.243 (1987) 1222.
Tramontana, M., Del Bianco, E., Ziche, M., Santicioli, P., Maggi, C. A., and Geppetti, P., Pharmac. Res. Commun.23 (1991) 285.
Spillantini, M. G., Panconesi, A., Del Bianco, P. L., and Sicuteri, F., Neuropeptides8 (1986) 111.
Skrabaneck, P., Cannon, D., Kirrane, J., Legge, D., and Powell, D., Ir. J. med. Sci.145 (1976) 399.
Berger, H., Fencher, K., Albrecht, E., and Niedrich, H., Biochem. Pharmac.28 (1979) 3173.
Levin, E. G., and Loskvtoff, D. J., J. Cell Biol.94 (1982) 631.
Loesch, A., and Burnstock, P., Anat. Embryol.178 (1988) 137.
Maggi, C. A., Patacchini, R., Perretti, F., Tramontana, M., Manzini, S., Geppetti, P., and Santicioli, P., Naunyn-Schmiedebergs Arch. Pharmak.342 (1990) 78.
Isolani, D., Cappugi, P., Lotti, T., Senesi, C., Urso, C., and Panconesi, E. in: Aspetti farmacologici e funzionali dei neuropeptidi: incontro nazionale, p. 33. Eds M. Ziche and P. Geppetti. University Florence, 1990.
Sicuteri, F., Fanciullacci, M., Franchi, G., and Michelacci, S., Experientia19 (1963) 44.
Sicuteri, F., Franchi, G., and Anselmi, B., Settim. med.50 (1962) 19.
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Fanciullacci, M., Fedi, S., Alessandri, M. et al. Substance P-induced fibrinolysis in the forearm of healthy humans. Experientia 49, 242–244 (1993). https://doi.org/10.1007/BF01923533
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DOI: https://doi.org/10.1007/BF01923533