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The possible role of phospholipase A2 in cardiac membrane destabilization under calcium overload conditions

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Summary

The mechanism of spontaneous diastolic depolarizations induced by different Ca2+ overloading conditions (ouabain toxicity, calcium ionophore A23187, O-K, high Ca2+ solution) in mammalian working myocardium fibres was studied with conventional microelectrode technique and pharmacological approach.

Antagonistic properties of antiphospholipase-A2 (PL A2)-active compounds (dexamethasone and indomethacin) were tested. Membrane oscillation in Ca2+ overload conditions were shown to be eliminated or largely protected by both anti-inflammatory agents. There was no influence of the compounds on electrical parameters and ion currents in intact mammalian and amphibian myocardium. The data obtained suggested that modulation of Ca2+-dependent PL A2 activity may contribute significantly to membrane destabilization due to Ca2+ overload of cardiac cells. An analogous membrane destabilizing action of exogenous PL A2 observed in Langendorff-perfused guinea pig heart is in favour of the hypothesis introduced.

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Author notes

  1. M. E. Saxon

    Present address: Department of Medical Biophysics, Institute of Biological Physics of the Academy of Sciences of the U.S.S.R., 142292, Pushchino, USSR

Authors and Affiliations

  1. Department of Medical Biophysics, Institute of Biological Physics of the Academy of Sciences of the U.S.S.R., 142292, Pushchino, USSR

    A. K. Filippov & U. I. Porotikov

Authors
  1. M. E. Saxon
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  2. A. K. Filippov
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  3. U. I. Porotikov
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Saxon, M.E., Filippov, A.K. & Porotikov, U.I. The possible role of phospholipase A2 in cardiac membrane destabilization under calcium overload conditions. Basic Res Cardiol 79, 668–678 (1984). https://doi.org/10.1007/BF01908384

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  • DOI: https://doi.org/10.1007/BF01908384

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