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Injection of catalytic subunit of cAMP-dependent protein kinase into isolated cardiac myocytes

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Abstract

Cyclic adenosine 3′,5′-monophosphate (cAMP) or the free catalytic subunit (C) of the cAMP-dependent protein kinase were pressure injected into single guinea pig ventricular cells. The following results were obtained:

  1. 1.

    Injection of cAMP prolonged the action potential and shifted the action potential plateau to a more positive level. Under voltage clamp, cAMP injection increased the amplitude of the slow inward calcium current (I si).

  2. 2.

    Injection of C permanently prolonged the action potential and enhanced the amplitude ofI si by a factor of 2–4, depending on the amount of injected C. In the current-voltage relations the potential of maximumI si and the apparent current reversal did not change.

  3. 3.

    After maximum prolongation of the action potential due to repeated injections of C, even high concentrations of adrenaline did not further change the configuration of the action potential.

  4. 4.

    In many experiments transient depolarizations appeared after the injection. Correspondingly, under voltage clamp transient inward currents occurred.

  5. 5.

    C injection increased both the time-dependent and time-independent potassium outward current.

  6. 6.

    In response to injection of the catalytic subunit, the isotonic contraction was larger in amplitude and relaxation was faster.

It is concluded that the cAMP-dependent protein kinase increases the slow inward calcium current in the heart, presumably by phosphorylation of some membrane proteins.

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Author notes

  1. G. Brum

    Present address: Departamento de Biofisica, Facultad de Medicina, Montevideo, Uruguay

Authors and Affiliations

  1. H. Physiologisches Institut der Universität des Saarlandes, D-6650, Homburg/Saar

    G. Brum, W. Osterrieder & W. Trautwein

  2. Pharmakologisches Institut der Universität Heidelberg, D-6690, Heidelberg, Federal Republic of Germany

    V. Flockerzi & F. Hofmann

Authors
  1. G. Brum
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  2. V. Flockerzi
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  3. F. Hofmann
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  4. W. Osterrieder
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  5. W. Trautwein
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Additional information

This work was supported by the Deutsche Forschungsgemeinschaft SFB 38, Membranforschung, project G, and DFG Ho 579/6-1 and Fonds der Chemischen Industrie

Dr. Brum is a recipient of a fellowship from the Deutscher Akademischer Austauschdienst

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Brum, G., Flockerzi, V., Hofmann, F. et al. Injection of catalytic subunit of cAMP-dependent protein kinase into isolated cardiac myocytes. Pflugers Arch. 398, 147–154 (1983). https://doi.org/10.1007/BF00581064

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  • DOI: https://doi.org/10.1007/BF00581064

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