Zusammenfassung
Mit Hilfe der Saccharose-Doppeltrennwandmethode wurden „Voltage-clamp“-Untersuchungen an feinen Frosch-Vorhof-Trabekeln durchgeführt. Sie zeigen:
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einen initialen Natrium-Strom als Funktion des Potentials und der Zeit, der durch TTX blockierbar ist. Seine Aktivierung wird durch verstärkte Depolarisation beschleunigt. Seine Inaktivierung verläuft in zwei Phasen. Die erste, schnelle hat eine Zeitkonstante von der Größenordnung 1 msec. Die zweite ist um mehrere Größenordnungen langsamer
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eine verzögerte Gleichrichtung, welche eine anomale Gleichrichtung überlagert, so daß die stationären Strom-Spannungskurven S-förmig verlaufen. Die verzögerte Gleichrichtung wird von TEA nur wenig vermindert
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negative (Einwärts-) Ströme bei hyperpolarisierenden Impulsen, die zeitabhängig abnehmen und die durch TEA stark vermindert werden, was auf eine hohe Kalium-Leitfähigkeit zu Beginn des Hyperpolarisationsimpulses schließen läßt.
Diese Befunde bestätigen diejenigen von McAllister and Noble (1966, 1967) bezüglich einer verzögerten Gleichrichtung in Herzmuskelfasern. In bezug auf das Na-System sind sie bis zu einem gewissen Grade übereinstimmend mit den Befunden von Giebisch u. Weidmann (1967) und mit der Hypothese von Brady u. Woodbury (1960). Sie können qualitativ den Verlauf des Aktionspotentials erklären.
Summary
Voltage clamp experiments with the double sucrose gap technique performed on frog heart atrial trabecles show:
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an initial sodium current as function of potential and time, blocked by TTX. Its activation is accelerated with increased depolarization. Its inactivation has a rapid phase with a time constant of the order of a millisecond, followed by a very slow phase
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a delayed rectification, superimposed on anomalous rectification. Steady state current voltage curves have sigmoid shape. The delayed currents are only very little reduced by TEA
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negative currents decreasing with time during hyperpolarizing voltage steps. They are strongly reduced by TEA which suggests a high potassium conductance at the beginning of the steps.
These results confirm the findings of McAllister and Noble (1966, 1967) concerning the existence of a delayed rectification in cardiac muscle fibres. With respect to the sodium system they are to a certain extent in agreement with the ones of Giebisch and Weidmann (1967) and with the hypothesis of Brady and Woodbury (1960). They can qualitatively explain the time course of the action potential.
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This work was in part supported by the Deutsche Forschungsgemeinschaft, Bad Godesberg.
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Rougier, O., Vassort, G. & Stämpfli, R. Voltage clamp experiments on frog atrial heart muscle fibres with the sucrose gap technique. Pflügers Arch 301, 91–108 (1968). https://doi.org/10.1007/BF00362729
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DOI: https://doi.org/10.1007/BF00362729