Skip to main content
SpringerLink
Log in

Ultrastructural demonstration of nerve endings containing a substance related to growth hormone-releasing factor in the guinea-pig paraventricular nucleus

  • Published:
Cell and Tissue Research Aims and scope Submit manuscript

Summary

By means of a preembedding immuno-electronmicroscopic technique, a large number of nerve endings containing a substance related to human growth hormonereleasing factor (hGRF) have been demonstrated in the paraventricular nucleus of the guinea pig. They made synaptic contacts primarily with dendritic shafts: 80% of these contacts were symmetrical. The immunoprecipitate was located mainly in large granules and around small clear vesicles. These findings suggest that a peptide related to hGRF may play a role in neural communication in the paraventricular nucleus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bai FL, Yamano M, Shiotani Y, Emson PC, Smith AD, Powell JF, Tohyama M (1985) An arcuato-paraventricular and dorsomedial hypothalamic neuropeptide containing system which lacks noradrenaline in the rat. Brain Res 331:172–175

    PubMed  Google Scholar 

  • Beauvillain JC, Tramu G, Croix D, Poulain P (1985) Etude à l'échelle ultrastructurale de différentes terminaisons nerveuses peptidergiques dans l'éminence médiane, l'hypothalamus dorsal et le septum. Ann Endocrinol 46:9–17

    Google Scholar 

  • Bloch B, Brazeau P, Ling N, Bohlen P, Esch F, Wehrenberg WB, Benoit R, Bloom F, Guillemin R (1983) Immunohistochemical detection of growth hormone-releasing factor in brain. Nature 301:607–608

    PubMed  Google Scholar 

  • Bloom FE, Battenberg EL, Rivier J, Vale W (1982) Corticotropin releasing factor CRF: immunoreactive neurones and fibers in rat hypothalamus. Regul Pept 4:43–48

    PubMed  Google Scholar 

  • Bohlen P, Brazeau P, Bloch B, Ling N, Gaillard R, Guillemin R (1983) Human hypothalamic growth hormone releasing factor (GRF): evidence for two forms identical to tumor derived GRF-44-NH2 and GRF-40. Biochem Biophys Res Commun 114:930–936

    PubMed  Google Scholar 

  • Bugnon C, Couget A, Fellmann D, Clavequin MC (1983) Immunocytochemical demonstration of a novel peptidergic neurone system in the cat brain with an anti-growth hormone-releasing factor serum. Neurosci Lett 38:131–137

    PubMed  Google Scholar 

  • Bugnon C, Fellmann D, Couget A, Cardot J (1982) Corticoliberin in rat brain: immunocytochemical identification and localization of a novel neuroglandular system. Neurosci Lett 30:25–30

    PubMed  Google Scholar 

  • Colonnier M (1981) The electron microscopic analysis of the neuronal organization of the cerebral cortex. In: Schmitt F, Worden G, Adelmo G, Denis SG (eds) The organization of the cerebral cortex. Cambridge MIT Press, pp 122–152

  • Eccles JC (1964) The physiology of synapses. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Elde R, Hökfelt T, Johansson O, Schultzberg M, Efendic S, Luft R (1978) Cellular localization of somatostatin. Metabolism [Suppl] 27:1151–1159

    Google Scholar 

  • Fellmann D, Gouget A, Bugnon C (1983) Mise en évidence d'un nouveau système neuronal peptidergique immunoréactif à un immunsérum anti-hp GRF44 dans le cerveau du Lérot (Eliomys quercinus). C R Acad Sci Paris 296:487–492

    Google Scholar 

  • Guillemin R, Brazeau P, Bohlen P, Esch F, Ling N, Wehrenberg WB (1982) Growth hormone releasing factor from a human pancreatic tumor that caused acromegaly. Science 218:585–587

    PubMed  Google Scholar 

  • Imaki T, Shibasaki T, Hotta M, Masuda A, Demura H, Shizume K, Ling N (1985) The satiety effect of growth hormone-releasing factor in rats. Brain Res 340:186–188

    PubMed  Google Scholar 

  • Jacobowitz DM, Schulte H, Chrousos GP, Loriaux DL (1983) Localization of GRF-like immunoreactive neurons in the rat brain. Peptides 4:521–524

    PubMed  Google Scholar 

  • Kahn D, Abrams GM, Zimmerman EA, Corraway R, Leeman SE (1980) Neurotensin neurons in the rat hypothalamus: an immunohistochemical study. Endocrinology 107:47–54

    PubMed  Google Scholar 

  • Kiss JZ, Palkovits M, Zaborzky L, Tribollet E, Szabo D, Makara GB (1983) Quantitative histological studies on the hypothalamic paraventricular nucleus in rats: number of cells and synaptic boutons. Brain Res 262:217–224

    PubMed  Google Scholar 

  • Lechan RM, Jackson IMD (1982) Immunohistochemical localization of thyrotropin-releasing hormone in the rat hypothalamus and pituitary. Endocrinology 115:55–65

    Google Scholar 

  • Leidy JW, Robbins RJ (1986) Regional distribution of human growth hormone releasing hormone in the human hypothalamus by radioimmunoassay. J Clin Endocrinol Metab 62:372–378

    PubMed  Google Scholar 

  • Ling N, Esch F, Bohlen P, Brazeau P, Wehrenberg WB, Guillemin R (1984) Isolation, primary structure, and synthesis of human hypothalamic somatocrinin: growth hormone-releasing factor. Proc Natl Acad Sci USA 81:4302–4306

    PubMed  Google Scholar 

  • Merchenthaler I, Vigh S, Schally AV, Petrusz P (1984) Immunocytochemical localization of growth hormone-releasing factor in the rat hypothalamus. Endocrinology 114:1082–1085

    PubMed  Google Scholar 

  • Poulain P, Carette B (1986) Effects of GRF on paraventricular neurons in slices of guinea-pig hypothalamus. Brain Res 371:355–359

    PubMed  Google Scholar 

  • Rivier J, Spiess J, Thorner M, Vale W (1982) Characterization of a growth hormone-releasing factor from a human pancreatic islet tumour. Nature 300:276–278

    PubMed  Google Scholar 

  • Sawchenko PE, Swanson LW, Rivier J, Vale WW (1985) The distribution of growth hormone-releasing factor (GRF) immunoreactivity in the central nervous system of the rat: An immunohistochemical study using antisera directed against rat hypothalamic GRF. J Comp Neurol 237:100–115

    PubMed  Google Scholar 

  • Smith RM, Howe PRC, Oliver JR, Willoughby JO (1984) Growth hormone releasing factor immunoreactivity in rat hypothalamus. Neuropeptides 4:109–115

    PubMed  Google Scholar 

  • Stanley BG, Leibowitz SF (1985) Neuropeptide Y injected in the paraventricular hypothalamus: A powerful stimulant of feeding behavior. Proc Natl Acad Sci USA 82:3940–3943

    PubMed  Google Scholar 

  • Tramu G, Beauvillain JC, Pillez A, Mazzuca M (1983a) Presence d'une substance immunologiquement apparantée à la somatolibérine extraite d'une tumeur pancréatique humaine (hp GRF) dans des neurones de l'aire hypophysiotrope du Cobaye et du Rat. C R Acad Sci Paris 297:435–440

    Google Scholar 

  • Tramu G, Croix D, Pillez A (1983b) Ability of the CRF immunoreactive neurons of the paraventricular nucleus to produce vasopressin-like material. Neuroendocrinology 37:467–469

    PubMed  Google Scholar 

  • Vaccarino FJ, Bloom FE, Rivier J, Vale W, Koob GF (1985) Stimulation of food intake in rats by centrally administered hypothalamic growth hormone releasing factor. Nature 314:167–168

    PubMed  Google Scholar 

  • Varro A, Lonovics J, Hajnal F, Somogyi P (1983) Subcellular localization of bioactive cholecystokinin-like peptides in the rat cerebral cortex. J Neurochem 41:890–893

    PubMed  Google Scholar 

  • Wehrenberg W, Ling N, Brazeau P, Esch F, Bohlen P, Baird A, Ying SW, Guillemin R (1982) Somatocrinin, growth hormonereleasing factor, stimulates secretion of growth hormone in anesthetized rats. Biochem Biophys Res Commun 109:382–387

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. U 156 INSERM, Lille, France

    J. C. Beauvillain, G. Tramu & M. Mazzuca

Authors
  1. J. C. Beauvillain
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. Tramu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Mazzuca
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Beauvillain, J.C., Tramu, G. & Mazzuca, M. Ultrastructural demonstration of nerve endings containing a substance related to growth hormone-releasing factor in the guinea-pig paraventricular nucleus. Cell Tissue Res. 248, 223–226 (1987). https://doi.org/10.1007/BF01239984

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01239984

Key words

Search

Navigation