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Distribution of somatostatin-28 (1-12), calcitonin gene-related peptide, and substance P in the squirrel monkey brainstem: an immunocytochemical study

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Abstract

Using an immunocytochemical technique, we have studied the distribution of fibers and cell bodies containing somatostatin-28 (1-12) [SOM-28 (1-12)], calcitonin gene-related peptide (CGRP), and substance P (SP) in the brainstem of Saimiri sciureus. The distribution of the peptidergic cell bodies was very restricted: perikarya containing SOM-28 (1-12) were only observed in the substantia grisea centralis, while no immunoreactive cell bodies containing CGRP or SP were visualized. Fibers containing SOM-28 (1-12), CGRP, or SP were widely distributed in the brainstem: immunoreactive fibers containing SOM-28 (1-12) showed the most widespread distribution and were the most abundant. The distribution of SOM-28 (1-12)-, CGRP- or SP-immunoreactive fibers was very similar. Colocalization of immunoreactive fibers containing SOM-28 (1-12), CGRP or SP was observed in many brainstem nuclei. A neuroanatomical relationship between CGRP- and SP-immunoreactive fibers was observed, although this relationship was less marked for SOM-28 (1-12) and SP and lower still for SOM-28 (1-12) and CGRP. The widespread distribution of the peptidergic fibers suggests that the studied neuropeptides are involved in many physiological actions.

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Abbreviations

Ann:

Nucleus annularis

AS:

Aquaeductus Sylvii

BC:

Brachium conjunctivum

BP:

Brachium pontis

Cd:

Nucleus caudatus

CD:

Nucleus cochlearis dorsalis

Cn:

Nucleus cuneatus

CnL:

Nucleus cuneatus lateralis

CM:

Centrum medianum thalami

CoS:

Colliculus superior

CT:

Corpus trapezoideum

CV:

Nucleus cochlearis ventralis

DBC:

Decussatio brachii conjunctivi

DG:

Nucleus dorsalis tegmenti (Gudden)

DPy:

Decussatio pyramidum

DR:

Nucleus dorsalis raphae

DV:

Nucleus dorsalis nervi vagi

FC:

Funiculus gracilis

FRM:

Formatio reticularis myelencephali

FRPo:

Formatio reticularis pontis oralis

FRTM:

Formatio reticularis tegmenti mesencephali

G:

Nucleus gracilis

GC:

Substantia grisea centralis

GCv:

Substantia grisea centralis, pars ventralis

GL:

Corpus geniculatum lateralis

GM:

Corpus geniculatum medialis

GPo:

Griseum pontis

IP:

Nucleus interpeduncularis

LC:

Locus coeruleus

LM:

Nucleus lemnisci lateralis

LP:

Nucleus lateralis posterior thalami

MD:

Nucleus medialis dorsalis thalami

NCI:

Nucleus colliculi inferioris

NCS:

Nucleus centralis superior tegmenti

NIII:

Nucleus nervi oculomotorii

nIV:

Nervus trochlearis

NIV:

Nucleus nervi trochlearis

NR:

Nucleus ruber

NSv:

Nucleus tractus spinalis nervi trigemini

NTS:

Nucleus tractus solitarii

nV:

Nervus trigemini

NVI:

Nucleus nervi abducentis

NVII:

Nucleus nervi facialis

nVIIIa:

Nervus acusticus

NXII:

Nucleus nervi hypoglossi

OI:

Nucleus olivaris inferior

OIm:

Nucleus olivaris inferior, accessorius medialis

OIp:

Nucleus olivaris inferior principalis

OS:

Nucleus olivaris superior

PbL:

Nucleus parabrachialis lateralis

PbM:

Nucleus parabrachialis medialis

pf:

Nucleus parafascicularis thalami

Pp:

Nucleus praepositus

PTc:

Nucleus praetectalis

PuO:

Nucleus peripeduncularis oralis thalami

Pv:

Nucleus principalis n. trigemini

Py:

Tractus pyramidalis

RL:

Nucleus reticularis lateralis myelencephali

RPm:

Nucleus reticularis paramedianus myelencephali

RTP:

Nucleus reticularis tegmenti pontis

SNc:

Substantia nigra, pars compacta

SNd:

Substantia nigra, pars diffusa

TSc:

Tractus tectospinalis nervi trigemini

TTS:

Tractus tectospinalis

VesI:

Nucleus vestibularis inferior

VesL:

Nucleus vestibularis lateralis

VesM:

Nucleus vestibularis medialis

VesS:

Nucleus vestibularis superior

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Acknowledgements

This work has been supported by the Universidad de Santander UDES, Bucaramanga (Colombia). The authors wish to thank Professor Gérard Tramu (Université de Bordeaux I, France) for the gift of primary antibodies, and the language service of the Universidad de Santander UDES (Colombia) for supervising the English text. This work is dedicated to Professor Julio Villalobos (Universidad del Valle, Cali, Colombia).

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Authors and Affiliations

  1. Universidad de Santander UDES, School of Medicine, Neuroscience Group, Bucaramanga, Colombia

    E. Duque-Díaz

  2. Institute of Neuroscience of Castilla y León (INCYL), Laboratory of Neuroanatomy of the Peptidergic Systems (Lab. 14), University of Salamanca, Salamanca, Spain

    R. Coveñas

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  1. E. Duque-Díaz
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  2. R. Coveñas
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Correspondence to E. Duque-Díaz.

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Duque-Díaz, E., Coveñas, R. Distribution of somatostatin-28 (1-12), calcitonin gene-related peptide, and substance P in the squirrel monkey brainstem: an immunocytochemical study. Anat Sci Int 94, 86–100 (2019). https://doi.org/10.1007/s12565-018-0453-y

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