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Alkaline phosphatase activity in cultured meningioma cells

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Summary

The specific activity of alkaline phosphatase in cultured human meningioma cells varies over a relatively wide range. There is no correlation between the levels of activity and the histological type of meningioma from which the cultures were derived. The enzyme is heat-labile and is strongly inhibited byl-homoarginine, levamisole, and l-bromotetramisole, but unaffected byl-phenylalanine andl-phenylalanylglycylglycine. These findings indicate that meningioma cells synthesize the liver/bone/kidney form of alkaline phosphatase. In contrast to cultures derived from pituitary adenomas, glioblastomas, and astrocytomas in which prednisolone and/or sodium butyrate elicit a manifold increase of alkaline phosphatase activity, with meningioma cells the hormone causes only a slight augmentation in specific activity, and the fatty acid is ineffective. As with other cells producing the liver/bone/kidney enzyme form, no increase in activity occurs in meningioma cells growing in hyperosmolar medium.

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Authors and Affiliations

  1. Division of Neuropathology and Tissue Culture Laboratory, Dept. of Pathology, Montefiore Hospital and Medical Center, Albert Einstein College of Medicine, 111 East 210th Street, 10467, Bronx, NY, USA

    N. Takahara, F. Herz & A. Hirano

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  1. N. Takahara
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  2. F. Herz
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  3. A. Hirano
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Takahara, N., Herz, F. & Hirano, A. Alkaline phosphatase activity in cultured meningioma cells. Acta Neuropathol 57, 45–50 (1982). https://doi.org/10.1007/BF00688876

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  • DOI: https://doi.org/10.1007/BF00688876

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