Summary
In this paper the serotonin-containing neurons in the diencephalon and mesencephalon of the trout have been studied by immunocytochemistry. Serotonin-immunoreactive perikarya, fibres and terminals were visualized by using well-characterized antibodies to serotonin. Six areas could be demonstrated to contain serotonin-immunoreactive cellbodies. 1. A group of cells, situated ventral to the commissura posterior; 2. A region within the confines of the nucleus recessi lateralis and a few scattered cells dorsally to this nucleus; 3. Neurons, situated ventral to the recessus lateralis hypothalami, within the nucleus tuberis inferior; 4. Cells which are situated within the borders of the nucleus recessi posterioris; 5. A number of cells, situated within the nucleus raphes superior and finally: 6. Perikarya, located dorsolateral to the nucleus raphes superior. The following three regions showed a very high density of serotonin-immunoreactive fibres and terminals: A. the most dorsal portion of the nucleus diffusus tori lateralis; B. the area dorsal to the recessus lateralis and C. the area surrounding the recessus posterior.
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References
Baumgarten HG (1972) Biogenic monoamines in the cyclostome and lower vertebrate brain. Histochem Cytochem 4:1–90
Baumgarten HG, Braak H (1967) Catecholamine im Hypothalamus vom Goldfish (Carassius auratus). Z Zellforsch 80:246–263
Bergquist H (1932) Zur morphologie des Zwischenhirns bei niederen Wirbeltieren. Stockholm, pp 303
Bertler A, Falde B, Mecklenburg CV (1963) Monoaminergic mechanisms in special ependymal areas in Salmo virideus. Gen Comp Endocrinol 3:685–686
Braak H (1967) Elektronenmikroskopische Untersuchungen an Catecholaminkernen im Hypothalamus vom Goldfish (Carassius auratus). Z Zellforsch 83:398–415
Braak H, Baumgarten HG (1967) 5-Hydroxytryptamin im Zentralnervensystem vom Goldfish (Carassius auratus). Z Zellforsch 81:416–432
Ekengren B (1975) Aminergic nuclei in the hypothalamus of the roach, Leuciscus rutilus. Cell Tissue Res 159:493–502
El Hawary MBE, Feldberg W, Lotti VJ (1967) Monoamine-oxydase inhibition: Effect on 5-hydroxytryptamine-output from perfused third ventricle and body-temperature. J Physiol (Lond) 188:131–140
Fremberg M, Veen van Th, Hartwig HG (1977) Formaldehyde-induced fluorescence in the telencephalon and diencephalon of the eel (Anguilla anguilla L.) Cell Tissue Res 176:1–22
Hafeez MA, Zerihun L (1976) Autoradiographic localization of 3H-5-HTP and 3H-5-HT in the pineal organ and circumventricular areas in the rainbow trout, Salmo gairdneri Richardson. Cell Tissue Res 170:61–76
l'Hermite A, Lefranc G (1972) Recherches sur les voies monoaminergiques de l'encéphale d'Anguilla vulgaris. Arch d'Anat Microsc 61:139–152
Honma S, Honma Y (1970) Histochemical demonstration of MA's in the hypothalamus of the lamprey and ice-goby. Bull Jpn Soc Sci Fish 36:125–134
Kah O, Chambolle P (1983) Serotonin in the brain of the goldfish, Carassius auratus. An immunocytochemical study. Cell Tissue Res 234:319–333
Knowles F, Vollrath L (1966) Neurosecretory innervation of the pituitary of the eels Anguilla and Conger. II. The structure and innervation of the pars distalis at different stages of the lifecycle. Phil Trans B 250:329–342
Kotrschal K, Adam H (1983) The aminergic system in the brain of Blennitis incognitus (Bath 1968) (Teleostei, Perciformes). Cell Tissue Res 229:403–409
Lefranc G, l'Hermite A, Tusques J (1969) Mise en évidence de neurones monoaminergiques par la technique de fluorescence dans l'encephal d'Anguilla. Cr Séanc Soc Biol 163:1193–1196
Lefranc G, l'Hermite AL, Tusques J (1970) Etude topographique et cytologique des différents noyaux monoaminergiques de l'encéphale d'Anguilla vulgaris. CR Soc Biol (Paris) 164:1629–1934
Miller RN (1940) The diencephalic cell masses of the teleost, Corydoras paleatus. J Comp Neurol 73:345–378
Müller H, Weiss J, Sterba G, Hoheiscl G (1974) To the relations between cerebrospinal fluid and lcn's. In: A Mitro (ed) Ependyma and neurohormonal regulation. Veda Publ House Slovak Acad Sci Bratislanar: pp 195–203
Owman C, Rüdeberg C (1970) Light-, fluorescence- and electron microscopic studies on the pineal organ of the pike, Esox lucius L. with special regard to 5-hydroxytryptamine. Z Zellforsch mikrosk-anat 107:522–550
Parent A, Dube L, Braford Jr MR, Northcutt RG (1978) The organization of monoamine-containing neurons in the brain of the sunfish (Lepomis gibbosus) as revealed by fluorescence microscopy. J Comp Neurol 182:495–516
Pease DC (1962) Buffered formaldehyde as a killing agent and primary fixative for electron microscopy. Anat Rec 142:342
Peter PE, Gill VE (1975) A stereotaxic atlas and technique for forebrain-nuclei of the goldfish, Carassius auratus. J Comp Neurol 159:69–101
Schnitzlein HN (1962) The habenula and the dorsal thalamus of some teleosts. J Comp Neurol 118:225–267
Steinbusch HWM, Nieuwenhuys R (1983) The raphe nuclei of the rat brain stem: a cytoarchitectonic and immunohistochemical study. In: P Emson (ed) Chemical neuroanatomy. Raven Press New York, pp 131–207
Steinbusch HWM, Verhofstad AAJ, Joosten H (1978) Immunofluorescent staining of serotonin in the central nervous system. In: P Simon (ed) Advances in pharmacology and therapeutics. Vol 2 Neurotransmitters, p 151
Steinbusch HWM, Nieuwenhuys R, Verhofstad AAJ (1979) Analyse van het serotonerge neuronsysteem in het central zenuwstelsel van de prik (Lampetra fluviatilis) en de rat met behulp van immunofluorescentie. Ned T Geneesk 123:2046
Steinbusch HWM, Kooy van der D, Verhofstad AAJ, Pellegrino A (1980) Serotonergic and non-serotonergic projections from the nucleus raphe dorsalis to the caudate-putamen complex in the rat, studied by a combined immunofluorescence and fluorescent retrograde axonal labeling technique. Neurosci Lett 19:137–142
Steinbusch HWM, Nieuwenhuys R, Verhofstad AAJ, Kooy van der D (1981a) The nucleus raphe dorsalis of the rat and its projections upon the caudato-putamen. A combined cytoarchitectonic, immunohistochemical and retrograde transport study. J Physiol 77:157–174
Steinbusch HWM, Verhofstad AAJ, Penke B, Varga J, Joosten HWJ (1981b) Immunohistochemical characterization of monoamine-containing neurons in the rat and the lamprey Lampetra fluviatilis. Acta Histochem Supp 24:107–122
Terlou M, Ekengren B, Hiemstra K (1978) Localization of monoamines in the forebrain of two salmonid species, with special reference to the hypothalamo-hypophysial system. Cell Tissue Res 190:417–434
Urano A (1971a) Monoamine-oxidase in the hypothalamo-hypophysial region of the teleosts, Anguilla japonica and Oryzias latipes. Z Zellforsch 114:83–94
Urano A (1971b) MAO in the hypothalamo-hypophysial region of the brown smooth dogfish, Triakis scyllia. Endocrinol. Jpn 18:37–46
Vigh B, Vigh-Teichmann I (1973) Comparative ultrastructure of the cerebrospinal fluid-contacting neurons. Int Rev Cytol 35:189–251
Watson SJ (1980) The distribution of aminergic neurones and their projections in the brain of the teleost, Myoxocephalus scorpius. Cell Tissue Res 208:299–312
Wilson JF, Dodd JM (1973) Distribution of monoamines in the diencephalon and pituitary of the dogfish, Scyliorhinus canicula 1. Z Zellforsch 137:451–469
Zambrano D (1970) The nucleus lateralis tuberis system of the gobiid fish Gillichtys mirabilis. I Ultrastructural and Histochemical characterization of the nucleus. Z Zellforsch 110:9–26
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Frankenhuis-van den Heuvel, T.H.M., Nieuwenhuys, R. Distribution of serotonin-immunoreactivity in the diencephalon and mesencephalon of the trout, Salmo gairdneri . Anat Embryol 169, 193–204 (1984). https://doi.org/10.1007/BF00303149
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DOI: https://doi.org/10.1007/BF00303149