Skip to main content
SpringerLink
Log in

Hyper-UV-sensitive yeast I: Isolation and properties of two such mutants

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

By using the UV-sensitive yeast mutant uvs-1 (Nakai and Matsumoto, 1967) as starting material, a series of hypersensitive strains have been isolated. Two of these strains are described here. In addition to being hyper-UV-sensitive, they are also sensitive to gamma radiation and one is sensitive to visible light; a characteristic which was first observed as an apparent failure to photoreactivate. Both strains are cytoplasmic petites. The value of this method of isolating strains which have progressively lost their dispensible repair functions is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Brendel, M., Khan, N. A., Haynes, R. H.: Common steps in the repair of alkylation and radiation damage in yeast. Molec. Gen. Genetics 106, 289–295 (1970).

    Google Scholar 

  • Cox, B. S., Parry, J. M.: The isolation, genetics and survival characteristics of ultraviolet light-sensitive mutants in yeast. Mutat. Res. 6, 37–55 (1968).

    Google Scholar 

  • Elkind, M. M., Sutton, H.: Lethal effects of visible light in a mutant strain of haploid yeast I: General dependencies. Arch. Biochem. 72, 84–95 (1957a).

    Google Scholar 

  • —: Lethal effects of visible light in a mutant strain of haploid yeast II: Absorption and action spectra. Arch. Biochem. 72, 96–111 (1957b).

    Google Scholar 

  • Kilbey, B. J., Smith, S. M.: Similarities between a UV-sensitive mutant of yeast and bacterial mutants lacking excision-repair ability. Molec. Gen. Genetics 104, 253–257 (1969).

    Google Scholar 

  • Nakai, S., Matsumoto, S.: Two types of radiation sensitive mutants in yeast. Mutat. Res. 4, 129–136 (1967).

    Google Scholar 

  • Snow, R.: Mutants of yeast sensitive to ultraviolet light. J. Bact. 94, 571–575 (1967).

    Google Scholar 

  • Zimmerman, F. K.: Sensitivity to methylmethanesulphonate and nitrous acid of ultraviolet light-sensitive mutants in Saccharomyces cerevisiae. Molec. Gen. Genetics 102, 247–256 (1968).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Genetics, Edinburgh University, EH9 3JN, Edinburgh, Scotland

    Alistair M. Brown & B. J. Kilbey

Authors
  1. Alistair M. Brown
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. J. Kilbey
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by E. Witkin

Rights and permissions

Reprints and permissions

About this article

Cite this article

Brown, A.M., Kilbey, B.J. Hyper-UV-sensitive yeast I: Isolation and properties of two such mutants. Molec. Gen. Genet. 108, 258–265 (1970). https://doi.org/10.1007/BF00283356

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00283356

Keywords

Search

Navigation