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Delayed Colony Formation in Diploid Cells of Various Genotypes after UV Light Irradiation

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Abstract

Experimental curves of the dependence of survival and delayed colony formation on UV light (254 nm) fluence for two wild-type strains of diploid yeast Saccharomyces cerevisiae capable of recovering from UV damage and their UV-sensitive mutants are presented. The dose–response curves were sigmoid for wild-type cells and rad6/rad6 and rad18/rad18 mutants, which were sensitive to UV irradiation by a factor of 2.2 and 1.5 for survival and 2.0 and 3.1 for delayed colony formation in comparison with the original strain (XS800). The T2 (rad2/rad2) strain was characterized by an exponential dose–response curve and was more sensitive to UV irradiation by a factor of 10.7 for survival and 7.0 for delayed colony formation compared to the original strain (T1). Delayed colony formation of all studied strains reached 100% with increasing UV light fluence. Unlike traditional representations, these data indicate that the delayed colony formation is mainly determined by cell ploidy and does not depend on the shape of the dose–response curves and UV sensitivity of cells.

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REFERENCES

  1. Ultraviolet radiation, Geneva: WHO, 1995. https://www.who.int/uv/en/.

  2. Shore, R.E., Overview of radiation-induced skin cancer in humans, Int. J. Radiat. Biol., 1990, vol. 57, no. 4, pp. 809—827.

    Article  CAS  PubMed  Google Scholar 

  3. Kielbassa, C., Roza, L., and Epe, B., Wavelength dependence of oxidative DNA damage induced by UV and visible light, Cancerogenesis, 1997, vol. 18, no. 4, pp. 811—816.

    Article  CAS  Google Scholar 

  4. Tolstorukov, I.I., Bliznik, K.M., and Korogodin, V.I., Mitotic instability of diploid yeast cells of Pichia pinus: 1. Spontaneous cleavage, Genetica (Moscow), 1979, vol. 15, no. 12, pp. 2140—2147.

    Google Scholar 

  5. Chang, W.P. and Little, J.B., Persistently elevated frequency of spontaneous mutations in progeny of CHO clones surviving X-irradiation: association with delayed reproductive death phenotype, Mutat. Res., 1992, vol. 270, no. 2, pp. 191—199.

    Article  CAS  PubMed  Google Scholar 

  6. Marder, B.A. and Morgan, W.F., Delayed chromosomal instability induced by DNA damage, Mol. Cell. Biol., 1993, vol. 13, no. 11, pp. 6667—6677.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Vorobtsova, I.E., Transgenic transfer of radiation-induced genome instability and the susceptibility to carcinogenesis, Vopr. Onkol., 2008, vol. 54, no. 4, pp. 490—493.

    CAS  PubMed  Google Scholar 

  8. Kapul’tsevich, Yu.G., Korogodin, V.I., and Petin, V.G., Analysis of radiobiological reactions of yeast cells: 1. Survival curves and the grow-up effect, Radiobiologiya, 1972, vol. 12, no. 2, pp. 267—271.

    Google Scholar 

  9. Korogodin, V.I., Bliznik, K.M., Kapultcevich, Yu.G., et al., Cascade mutagenesis: regularities and mechanisms, Proceedings of the 2nd International N.W. Timofeef-Ressovsky Conference, Dubna, 2007, vol. 1, pp. 419—447.

  10. Evstratova, E.S., Pereklad, O.V., and Khryachkova, A.V., RBE of α-particles for delayed production of colonies by irradiated yeast cells, Radiats. Biol. Radioekol., 2016, vol. 56, no. 5, pp. 481—486.

    CAS  Google Scholar 

  11. Evstratova, E.S., Pereklad, O.V., and Petin, V.G., Dependence of radiation-induced genetic instability on the yeast cell ploidy, Radiat. Risk, 2016, vol. 25, no. 4, pp. 80—89.

    Article  Google Scholar 

  12. Evstratova, E.S., Khryachkova, A.V., Zhurakovskaya, G.P., et al., UV-induced genetic instability of yeast cells, Radiats. Biol. Radioekol., 2018, vol. 58, no. 3, pp. 245—250.

    Google Scholar 

  13. Petin, V.G., Pereklad, O.V., Nili, M., et al., Yeast cells retain a memory of their original radiation-induced insult, J. Radiat. Ind., 2008, vol. 2, no. 2, pp. 59—64.

    Google Scholar 

  14. Petin, V.G., Zhurakovskaya, G.P., and Komarova, L.N., Radiobiologicheskie osnovy sinergicheskogo vzaimodeistviya v biosfere (Radiobiological basis of Synergistic Interaction in the Biosphere), Moscow: GEOS, 2012.

  15. Melloni, E., Marchesini, R., Emanuelli, H., Fava, G., et al., Hyperthermal effects in phototherapy with hematoporphyrin derivative sensitization, Tumori, 1984, vol. 70, no. 4, pp. 321—325.

    Article  CAS  PubMed  Google Scholar 

  16. Waldow, S.M. and Dougherty, T.J., Interaction of hyperthermia and photoradiation therapy, Radiat. Res., 1984, vol. 97, no. 2, pp. 380—385.

    Article  CAS  PubMed  Google Scholar 

  17. Evstratova, E.S. and Petin, V.G., The delayed appearance of haploid and homozygous diploid Saccharomyces cerevisiae yeast cells of wild-type and radiosensitive mutants surviving after exposure to gamma rays and alpha particles, J. Radiat. Res. Appl. Sci., 2018, vol. 11, no. 1, pp. 98—103.

    Article  CAS  Google Scholar 

  18. Korogodin, V.I., Bliznik, K.M., Kapul’tsevich, Yu.G., and Petin, V.G., Assessment of mitotic recombination frequency in clones growing from irradiated cells, Radiobiologiya, 1974, vol. 14, no. 5, pp. 681—685.

    CAS  Google Scholar 

  19. Chang, W.P. and Little, J.B., Evidence that DNA double-strand break initiate the phenotype of delayed reproductive death in Chinese hamster ovary cells, Radiat. Res., 1992, vol. 131, no. 1, pp. 53—59.

    Article  CAS  PubMed  Google Scholar 

  20. Urushibara, A., Kodama, S., and Yokoya, A., Induction of genetic instability by transfer of a UV-A-irradiated chromosome, Mutat. Res., 2014, vol. 766, no. 1, pp. 29—34.

    Article  CAS  Google Scholar 

  21. Sheltzer, J.M., Blank, H.M., Pfau, S.J., et al., Aneuploidy drives genomic instability in yeast, Science, 2011, vol. 333, no. 6045, pp. 1026—1030.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Kaprin, A.D., Galkin, V.N., Zhavoronkov, L.P., et al., Synthesis of basic and applied research: the basis for ensuring a high level of scientific results and their implementation in medical practice, Radiat. Risk, 2017, vol. 26, no. 2, pp. 26—40.

    Article  Google Scholar 

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Funding

This work was carried out in the framework of implementing the state task of the Tsyb Medical Radiological Research Center, the branch of the National Medical Research Radiological Center of the Ministry of Health of Russia, for 2015–2017, the intermediate results of which were reflected in the generalizing publication [22]. This work was partly supported by the grant of the President MK-872.2018.4 (contract no. 075-02-2018-570).

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Authors and Affiliations

  1. Tsyb Medical Radiological Research Center, Branch of the National Medical Research Radiological Center of the Ministry of Health of the Russian Federation, 249036, Obninsk, Kaluga oblast, Russia

    E. S. Evstratova & V. G. Petin

  2. Konstantinov Petersburg Nuclear Physics Institute, National Research Center Kurchatov Institute, 188300, Gatchina, Leningrad oblast, Russia

    V. G. Korolev

Authors
  1. E. S. Evstratova
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  2. V. G. Korolev
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  3. V. G. Petin
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Correspondence to E. S. Evstratova.

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The authors declare that they have no conflict of interest. This article does not contain any research using animals as objects. This article does not contain any research involving human subjects as objects of research.

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Translated by K. Lazarev

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Evstratova, E.S., Korolev, V.G. & Petin, V.G. Delayed Colony Formation in Diploid Cells of Various Genotypes after UV Light Irradiation. Russ J Genet 55, 904–907 (2019). https://doi.org/10.1134/S1022795419070068

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  • DOI: https://doi.org/10.1134/S1022795419070068

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