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Mutational Specificity Analysis: Assay for Mutations in the Yeast SUP4-o Gene

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Yeast Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1163))

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Abstract

Mutational specificity analysis can yield valuable insights into processes that generate genetic change or maintain genetic stability. Powerful diagnostic tools for such analysis have been created by combining genetic assays for mutation with DNA sequencing. Here, steps for isolating spontaneous mutations in the yeast (Saccharomyces cerevisiae) suppressor tRNA gene SUP4-o as a prelude to sequence characterization are described (modifications of this protocol can be used to study induction of mutations by various physical or chemical agents). Mutations in SUP4-o are selected on drug-containing medium by virtue of their inactivation of suppressor activity. The small size, detailed knowledge of detectably mutable sites, and other features of the target gene facilitate subsequent analysis of these mutations.

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Authors and Affiliations

  1. Life and Environmental Sciences, Deakin University, 75 Pigdons Road, Waurn Ponds, VIC, 3216, Australia

    Bernard A. Kunz

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  1. Bernard A. Kunz
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Correspondence to Bernard A. Kunz .

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Editors and Affiliations

  1. Capital Normal University College of Life Sciences, Beijing, China

    Wei Xiao

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Kunz, B.A. (2014). Mutational Specificity Analysis: Assay for Mutations in the Yeast SUP4-o Gene. In: Xiao, W. (eds) Yeast Protocols. Methods in Molecular Biology, vol 1163. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0799-1_15

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  • DOI: https://doi.org/10.1007/978-1-4939-0799-1_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0798-4

  • Online ISBN: 978-1-4939-0799-1

  • eBook Packages: Springer Protocols

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