Skip to main content
SpringerLink
Log in

Physiological and biochemical studies of nuclear and extranuclear oligomycin-resistant mutants of Aspergillus nidulans

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

The biochemistry and physiology of a number of nuclear and one extranuclear oligomycin-resistant mutants of Aspergillus nidulans have been studied.

All the mutants showed at least a 50-fold increase in oligomycin resistance compared with the wild type, and all were cross-resistant to rutamycin. A number of the nuclear mutants also exhibited cross-resistance to venturicidin. None of the mutants were cross-resistant to DCCD, chloramphenicol or cycloheximide.

In a total of 6 mutants examined, including the extranuclear mutant, none possessed a mitochondrial ATPase with an in vitro oligomycin resistance greater than about 7 times that of the wild type.

The extranuclear mutant, strain OR6, exhibited impaired growth ability on drug-free media. On oligomycin, rutamycin or chloramphenicol, however, its impaired growth ability was not apparent. The extranuclear mutant also possessed an altered cytochrome spectrum.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Avner, P.R., Griffiths, D.E.: Oligomycin resistant mutants in yeast. FEBS Letters 10, 202–207 (1970)

    Article  CAS  PubMed  Google Scholar 

  • Avner, P.R., Griffiths, D.E.: Studies on energy-linked reactions. Isolation and characterisation of oligomycin-resistant mutants of Saccharomyces cerevisiae. Europ. J. Biochem. 32, 301–311 (1973a)

    Article  CAS  PubMed  Google Scholar 

  • Avner, P.R., Griffiths, D.E.: Studies on energy-linked reactions. Genetic analysis of oligomycin-resistant mutants of Saccharomyces cerevisiae. Europ. J. Biochem. 32, 312–321 (1973b)

    Article  CAS  PubMed  Google Scholar 

  • Bainbridge, B.W.: Macromolecular composition and nuclear division during spore germination in Aspergillus nidulans. J. gen. Microbiol. 66, 319–325 (1971)

    Article  CAS  PubMed  Google Scholar 

  • Bulos, B., Racker, E.: Partial resolution of the enzymes catalysing oxidative phosphorylation. XVII. Further resolution of the rutamycin-sensitive adenosine triphosphatase. J. biol. Chem. 243, 3891–3900 (1968)

    Article  CAS  PubMed  Google Scholar 

  • Dorn, G.L.: A revised map of the eight linkage groups of Aspergillus nidulans. Genetics 56, 619–631 (1967)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ephrussi, B.L.: Nucleo-cytoplasmic relations in microorganisms. Oxford: Clarendon Press 1953

    Google Scholar 

  • Fiske, C.H., Subbarow, Y.: The colourimetric determination of phosphorus. J. biol. Chem. 66, 375–400 (1925)

    Article  CAS  Google Scholar 

  • Fleischer, S., Fleischer, B.: Removal and binding of polar lipids in mitochondria and other membrane systems. III. C. Assay of Mg++-stimulated ATPase activity. In: Methods in enzymology (Estabrook, R.W., Pullman, M.E., eds.), vol. X, pp. 428–429. New York and London: Academic Press 1967

    Google Scholar 

  • Griffiths, D.E., Avner, P.R., Lancashire, W.E., Turner, J.R.: Studies of energy-linked reactions: isolation and properties of mitochondrial oligomycin-resistant, trialkyltin-resistant and uncoupler-resistant mutants of yeast. in: The biochemistry and biophysics of mitochondrial membranes (Azzone, G.F., Carafoli, E., Lehninger, A.L., Quagliariello, E., Siliprandi, N., eds.), pp. 505–521. New York and London: Academic Press 1972

    Google Scholar 

  • Handley, L., Caten, C.E.: Vegetative death: a mitochondrial mutation in Aspergillus amstelodami. Heredity 31, 136 (1973)

    Google Scholar 

  • Jagow, G. von, Weiss, H., Klingenberg, M.: Comparison of the respiratory chain of Neurospora crassa wild type and the mi-mutants mi-1 and mi-3. Europ. J. Biochem. 33, 140–157 (1973)

    Article  Google Scholar 

  • Lardy, H.A., Johnson, D., McMurray, W.C.: Antibiotics as tools for metabolic studies. I. A survey of toxic antibiotics in respiratory, phosphorylative and glycolytic systems, Arch. Biochem. Biophys. 78, 587–597 (1958)

    Article  CAS  PubMed  Google Scholar 

  • Lardy, H.A., Witonsky, P., Johnson, D.: Antibiotics as tools for metabolic studies. IV. Comparative effectiveness of oligomycins A, B, C and rutamycin as inhibitors of phosphoryl transfer reactions in mitochondria. Biochemistry (Wash.). 4, 552–554 (1965)

    Article  CAS  Google Scholar 

  • Linnane, A.W., Lamb, A.J., Christodoulou, C., Lukins, H.B.: The biogenesis of mitochondria. VI. Biochemical basis of the resistance of Saccharomyces cerevisiae toward antibiotics which specifically inhibit mitochondrial protein synthesis. Proc. nat. Acad. Sci. (Wash.) 59, 1288–1293 (1968)

    Article  CAS  Google Scholar 

  • Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)

    Article  CAS  PubMed  Google Scholar 

  • Mager, J.: Chloramphenicol and chlortetacycline inhibition of amino acid incorporation into proteins in a cell-free system from Tetrahymena pyriformis. Biochim. biophys. Acta (Amst.) 38, 150–152 (1960)

    Article  CAS  Google Scholar 

  • McCully, K.S., Forbes, E.: The use of p-fluorophenylalanine with “master strains” of Aspergillus nidulans for assigning genes to linkage groups. Genet. Res. 6, 352–359 (1965)

    Article  CAS  PubMed  Google Scholar 

  • Michel, R., Huet, P., Huet, M.: Action de la rutamycine (A272) sur les adénosinetriphosphatases mitochondriales hépatiques de rat. C. R. Soc. Biol (Paris) 158, 994–998 (1964)

    CAS  Google Scholar 

  • Mitchell, C.H., Bunn, C.L., Lukins, H.B., Linnane, A.W.: Studies on cross-resistance between inhibitors of mitochondrial oxidative phosphorylation and mitochondrial protein synthesis: a new insight into mitochondrial membrane structure. Proc. Aust. biochem. Soc. 4, 67 (1971)

    Google Scholar 

  • Mitchell, C.H., Bunn, C.L., Lukins, H.B., Linnane, A.W.: Biogenesis of mitochondria: 23. The biochemical and genetic characterisitics of two different oligomycin resistant mutants of Saccharomyces cerevisiae under the influence of cytoplasmic genetic modification. J. Bioenergetics 4, 161–178 (1973)

    Article  CAS  Google Scholar 

  • Nishimura, M., Ito, T., Chance, B.: Studies on bacterial photophosphorylation. III. A sensitive and rapid method of determination of photophosphorylation. Biochim. biophys. Acta (Amst.) 59, 177–182 (1962)

    Article  CAS  Google Scholar 

  • Ohnishi, T., Kawaguchi, K., Hagihara, B.: Preparation and some properties of yeast mitochondria. J. biol. Chem. 241, 1797–1806 (1966)

    Article  CAS  PubMed  Google Scholar 

  • Parker, J.H., Trimble, I.R., Jr., Mattoon, J.R.: Oligomycin resistance in normal and mutant yeast. Biochem. biophys. Res. Commun. 33, 590–595 (1968)

    Article  CAS  PubMed  Google Scholar 

  • Pontecorvo, G., Roper, J.A., Hemmons, L.M., MacDonald, K.D., Bufton, A.W.J.: The genetics of Aspergillus nidulans. Advanc. Genet. 5, 141–238 (1953)

    Article  CAS  Google Scholar 

  • Rowlands, R.T., Turner, G.: Nuclear and extranuclear inheritance of oligomycin resistance in Aspergillus nidulans. J. gen. Microbiol. 75, xix (1973a)

    Google Scholar 

  • Rowlands, R.T., Turner, G.: Nuclear and extranuclear inheritance of oligomycin resistance in Aspergillus nidulans. Molec. gen. Genet. 126, 201–216 (1973b)

    Article  CAS  PubMed  Google Scholar 

  • Shannon, C., Enns, R., Wheelis, L., Burchiel, K., Criddle, R.S.: Alterations in mitochondrial adenosine triphosphatase activity resulting from mutation of mitochondrial deoxyribonucleic acid. J. biol. Chem. 248, 3004–3011 (1973)

    Article  CAS  PubMed  Google Scholar 

  • Shaw, P.D.: Oligomycin complex, rutamycin and aurovertin. In: Antibiotics (Gottlieb, D., Shaw, P.D., eds.), vol. 1, pp. 585–610. Berlin-Heidelberg-New York: Springer 1967

    Chapter  Google Scholar 

  • Sherman, F., Slonimski, P.P.: Respiration-deficient mutants of yeast. II. Biochemistry. Biochim. biophys. Acta (Amst.) 90, 1–15 (1964)

    Article  CAS  Google Scholar 

  • Stekhoven, F.S., Waitkus, R.F., Moerkerk, H.Th.B. van: Identification of the d dicyclohexylcarbodiimide-binding protein in the oligomycin-sensitive adenosine triphosphatase from bovine heart mitochondria. Biochemistry (Wash.) 11, 1144–1150 (1972)

    Article  CAS  Google Scholar 

  • Stouthamer, A.H.: Determination and significance of molar growth yields. In: Methods in microbiology (Norris, J.R., Ribbons, D.W., eds.), vol. 1, pp. 629–663. London and New York: Academic Press 1969

    Google Scholar 

  • Stuart, K.D.: Cytoplasmic inheritance of oligomycin and rutamycin resistance in yeast. Biochem. biophys. Res. Commun. 39, 1045–1051 (1970)

    Article  CAS  PubMed  Google Scholar 

  • Tait, A.: Altered mitochondrial ribosomes in an erythromycin resistant mutant of Paramecium. FEBS Letters 24, 117–120 (1972)

    Article  CAS  PubMed  Google Scholar 

  • Thompson, R.Q., Hoehn, M.M., Higgens, C.E.: Crystalline antifungal antibiotic isolated from a strain of Streptomyces rutgersensis. In: Antimicrobial agent and chemotherapy-1961, pp. 474–480: published by the American Society for Microbiology 1961

  • Trinci, A.P.J.: A kinetic study of the growth of Aspergillus nidulans and other fungi. J. gen. Microbiol. 57, 11–24 (1969)

    Article  CAS  PubMed  Google Scholar 

  • Trinci, A.P.J., Gull, K.: Effect of actidione, griseofulvin and triphenyltin acetate on the kinetics of fungal growth. J. gen Microbiol. 60, 287–292 (1970)

    Article  CAS  PubMed  Google Scholar 

  • Turner, G.: Cycloheximide-resistant synthesis of mitochondrial-membrane components in Aspergillus nidulans. Europ. J. Biochem. 40, 200–206 (1973)

    Article  Google Scholar 

  • Wakabayashi, K.: Oligomycin resistance in yeast. II. Change in mitochondrial ATPase of a mutant and its genetic character. J. Antibiotics (Tokyo) 25, 475–476 (1972)

    Article  CAS  Google Scholar 

  • Wakabayashi, K., Gunge, N.: Extrachromosomal inheritance of oligomycin resistance in yeast. FEBS Letters 6, 302–304 (1970)

    Article  CAS  PubMed  Google Scholar 

  • Walter, P., Lardy, H.A., Johnson, D.: Antibiotics as tools for metabolic studies. X. Inhibition of phosphoryl transfer reaction in mitochondria by peliomycin, ossamycin and venturicidin. J. biol. Chem. 242, 5014–5018 (1967)

    Article  CAS  PubMed  Google Scholar 

  • Watson, K., Linnane, A.W.: Headpiece stalk particles lining membranes of mitochondria isolated from normal oligomycin-resistant mutants of Saccharomyces cerevisiae. J. Bioenergetics 3, 235–244 (1972)

    Article  CAS  Google Scholar 

  • Weiss, H., Jagow, G. von, Klingenberg, M., Bucher, Th.: Characterisation of Neurospora crassa mitochondria prepared with a grind-mill. Europ. J. Biochem. 14, 75–82 (1970)

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Bacteriology, The Medical School, University of Bristol, Bristol, England

    Robert T. Rowlands & Geoffrey Turner

Authors
  1. Robert T. Rowlands
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Geoffrey Turner
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by F. Kaudewitz

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rowlands, R.T., Turner, G. Physiological and biochemical studies of nuclear and extranuclear oligomycin-resistant mutants of Aspergillus nidulans. Molec. Gen. Genet. 132, 73–88 (1974). https://doi.org/10.1007/BF00268232

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00268232

Keywords

Search

Navigation