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Isolation of Chromosomal and Plasmid DNA from Bifidobacteria

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Bifidobacteria

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2278))

Abstract

Rapid and efficient protocols aimed at the isolation and purification of DNA for the purpose of downstream applications, such as cloning, PCR, Southern blotting, or sequencing, are essential for genetic, biochemical, and molecular biological analyses of a given bacterium. The protocols herein presented provide a robust and efficient method for the isolation of chromosomal and plasmid DNA from Bifidobacterium strains by organic extraction. The methods are simple, and the yield, purity, and quality of the DNA are adequate to perform various downstream applications including next-generation sequencing.

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Author information

Authors and Affiliations

  1. School of Microbiology and APC Microbiome Ireland, University College Cork, Cork, Ireland

    Maria Esteban-Torres, Rocio Sanchez-Gallardo & Douwe van Sinderen

  2. Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias, IPLA-CSIC, Villaviciosa, Spain

    Lorena Ruiz

  3. Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Oviedo, Asturias, Spain

    Lorena Ruiz

Authors
  1. Maria Esteban-Torres
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  2. Lorena Ruiz
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  3. Rocio Sanchez-Gallardo
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  4. Douwe van Sinderen
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Corresponding author

Correspondence to Maria Esteban-Torres .

Editor information

Editors and Affiliations

  1. Microbiome Ireland, School of Microbiology & APC, University College Cork, Cork, Ireland

    Douwe van Sinderen

  2. Laboratory of Probiogenomics, Department of Chemistry, Life Sciences & Environmental Sustainability, University of Parma, Parma, Italy

    Marco Ventura

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Esteban-Torres, M., Ruiz, L., Sanchez-Gallardo, R., van Sinderen, D. (2021). Isolation of Chromosomal and Plasmid DNA from Bifidobacteria. In: van Sinderen, D., Ventura, M. (eds) Bifidobacteria. Methods in Molecular Biology, vol 2278. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1274-3_3

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  • DOI: https://doi.org/10.1007/978-1-0716-1274-3_3

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1273-6

  • Online ISBN: 978-1-0716-1274-3

  • eBook Packages: Springer Protocols

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