Skip to main content
SpringerLink
Log in

Nuclear and extranuclear inheritance of oligomycin resistance in Aspergillus nidulans

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

A number of spontaneously-occurring, stable oligomycin-resistant mutants have been isolated in Aspergillus nidulans. Genetic characterisation showed that while most of the mutants examined were nuclear, one mutant was extranuclear as judged by several criteria. While the nuclear mutants showed no abnormalities on drug-free medium, the extranuclear mutant exhibited impaired growth ability. This character never segregated from the oligomycin-resistance character in any of the genetic experiments carried out, and appeared to be a secondary effect of the same mutation. The extranuclear genetic element coding for the oligomycin-resistance character was unable to co-exist in a stable fashion within the same mycelium as the wild type element, and they tended to segregate into sectors consisting almost wholly of one type or the other. The nuclear mutants showed incomplete dominance in heterozygous diploids, segregating fully resistant homozygous areas. All nuclear mutants mapped on linkage group VII.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Adouette, A., Beisson, J.: Evolution of mixed populations of genetically different mitochondria in Paramecium aurelia. Nature (Lond.) 235, 393–396 (1972)

    Google Scholar 

  • Arlett, C. F., Grindle, M., Jinks, J. L.: The red cytoplasmic variant of Aspergillus nidulans. Heredity 17, 197–209 (1962)

    Google Scholar 

  • Avner, P. R., Griffiths, D. E.: Oligomycin resistant mutants in yeast. FEBS Letters 10, 202–207 (1970)

    Google Scholar 

  • Avner, P. R., Griffiths, D. E.: Studies on energy-linked reactions. Isolation and characterisation of oligomycin-resistant mutants of Saccharomyces cerevisiae. Europ. J. Biochem., 32, 301–311 (1973a)

    Google Scholar 

  • Avner, P. R., Griffiths, D. E.: Studies on energy-linked reactions. Genetic analysis of oligomycin-resistant mutants of Saccharomyces cerevisiae. Europ. J. Biochem. 32, 312–321 (1973b)

    Google Scholar 

  • Beale, G. H., Knowles, J. K. C., Tait, A.: Mitochondrial genetics in Paramecium. Nature (Lond.) 235, 396–397 (1972)

    Google Scholar 

  • Coen, D., Deutsch, J., Netter, F., Petrochilo, E., Slonimski, P. P.: Mitochondrial genetics I-Methodology and phenomenology. In: Control of organelle development. Soc. exp. Biol. Symposia No. 24, 449–496 (1970)

  • Dorn, G. L.: A revised map of the eight linkage groups of Aspergillus nidulans. Genetics 56, 619–631 (1967)

    Google Scholar 

  • Edelman, M., Verma, I. M., Littauer, U.: Mitochondrial ribosomal RNA from Aspergillus nidulans: characterisation of a novel molecular species. J. molec. Biol. 49, 67–83 (1970)

    Google Scholar 

  • Forbes, E.: Use of mitotic segregation for assigning genes to linkage groups in Aspergillus nidulans. Heredity 13, 67–80 (1959)

    Google Scholar 

  • Grindle, M.: Nucleo-cytoplasmic interactions in the “red” cytoplasmic variant of Aspergillus nidulans. Heredity 19, 75–95 (1964)

    Google Scholar 

  • Handley, L., Caten, C. E.: Vegetative death: a mitochondrial mutation in Aspergillus amstelodami. Communicated at the 171st Meeting of the Genetical Society, April 1973

  • Jinks, J. L.: Cytoplasmic inheritance in fungi. In: Methodology in basic genetics (Ed. Burdette, W. J.), p. 325–354. San Francisco: Holden-Day Inc. 1963

    Google Scholar 

  • Lardy, H. A., Johnson, D., McMurray, W. C.: Antibiotics as tools for metabolic studies. I.A survey of toxic antibiotics in respiratory, phosphorylative and glycolytic systems. Arch. Biochem. Biophys. 78, 587–597 (1958)

    Google Scholar 

  • Lardy, H. A., Witonsky, P., Johnson, D.: Antibiotics as tools for metabolic studies. IV. Comparative effectiveness of oligomycins A, B, C and rutamycin as inhibitors of phosphoryl transfer reactions in mitochondria. Biochemistry (Wash.) 4, 552–554 (1965)

    Google Scholar 

  • Mackintosh, M. E., Pritchard, R. H.: The production and replica-plating of micro-colonies of Aspergillus nidulans. Genet. Res. 4, 320–322 (1963)

    Google Scholar 

  • McCully, K. S., Forbes, E.: The use of p-fluorophenylalanine with “master strains” of Aspergillus nidulans for assigning genes to linkage groups. Genet. Res. 6, 352–359 (1965)

    Google Scholar 

  • Michel, R., Huet, F., Huet, M.: Action de la rutamycine (A272) sur les a adénosinetriphosphatases mitochondriales hépatiques de rat. C. R. Soc. Biol. (Paris) 158, 994–998 (1964)

    Google Scholar 

  • Mitchell, C. H., Bunn, C. L., Lukins, H. B., Linnane, A. W.: Studies on cross-resistance between inhibitors of mitochondrial oxidative phosphorylation and mitochondrial protein synthesis: a new insight into mitochondrial membrane structure. Proc. Aust. biochem. Soc. 4, 67 (1971)

    Google Scholar 

  • Morpurgo, G.: Somatic segregation induced by p-fluorophenylalanine (PFP). Aspergillus News Letter 2, 10 (1961)

    Google Scholar 

  • Parker, J. H., Trimble, I. R., Jr., Mattoon, J. R.: Oligomycin resistance in normal and mutant yeast. Biochem. biophys. Res. Commun. 33, 590–595 (1968)

    Google Scholar 

  • Pontecorvo, G., Roper, J. A., Hemmons, L. M., MacDonald, K. D., Bufton, A. W. J.: The genetics of Aspergillus nidulans. Advanc. Genet. 5, 141–238 (1953)

    Google Scholar 

  • Roberts, C. F.: Silica-gel stock cultures of Aspergillus nidulans. Aspergillus News Letter 10, 29 (1969)

    Google Scholar 

  • Roper, J. A.: Production of heterozygous diploids in filamentous fungi. Experientia (Basel) 8, 14–15 (1952)

    Google Scholar 

  • Roper, J. A., Käfer, E.: Acriflavine-resistant mutants of Aspergillus nidulans. J. gen. Microbiol. 16, 660–667 (1957)

    Google Scholar 

  • Rowlands, R. T., Turner, G.: Nuclear and extranuclear inheritance of oligomycin-resistance in Aspergillus nidulans. J. gen. Microbiol. 75, xix (1973)

    Google Scholar 

  • Stuart, K. D.: Cytoplasmic inheritance of oligomycin and rutamycin resistance in yeast. Biochem. biophys. Res. Commun. 39, 1045–1051 (1970)

    Google Scholar 

  • Thompson, R. Q., Hoehn, M. M., Higgens, C. E.: Crystalline antifungal antibiotic isolated from a strain of Streptomyces rutgersensis. Antimicrobial Agents and Chemotherapy — 1961, 474–480, published by the American Society for Microbiology 1961

  • Upshall, A.: Nucleo-cytoplasmic interaction in fungi. MSc Thesis, University of Birmingham, England (1966)

  • Wakabayashi, K.: Oligomycin resistance in yeast. II. Change in mitochondrial ATPase of a mutant and its genetic character. Antibiot. (Tokyo) 25, 475–476 (1972)

    Google Scholar 

  • Wakabayashi, K., Gunge, N.: Extrachromosomal inheritance of oligomycin-resistance in yeast. FEBS Letters 6, 302–304 (1970)

    Google Scholar 

  • Waldron, C.: Cold-sensitive mutants of Aspergillus nidulans. Communicated at the 171st Meeting of the Genetical Society, April 1973

Download references

Author information

Authors and Affiliations

  1. Department of Bacteriology, The Medical School, University of Bristol, Bristol, England

    Robert T. Rowlands & Geoffrey Turner

Authors
  1. Robert T. Rowlands
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Geoffrey Turner
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by F. Kaudewitz

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rowlands, R.T., Turner, G. Nuclear and extranuclear inheritance of oligomycin resistance in Aspergillus nidulans . Molec. Gen. Genet. 126, 201–216 (1973). https://doi.org/10.1007/BF00267531

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00267531

Keywords

Search

Navigation