Abstract
The existence of a NADH oxidase catalyzed by a non-cytochrome containing pathway in membranes of free-living Rhizobium phaseoli was explored. This alternative electron transport route was distinguished from the cytochrome-oxidase linked pathway by its low affinity towards O2 (K \(\left( {K_{m_{app} } = 75 - 80{\text{ }}\mu M} \right)\), higher K m for NADH (75 μM), a hundred-fold lower sensitivity to quinarine inhibition, and resistance to UV (360 nm) photoinactivation. In addition to NADH, tetramethyl-p-phenylenediamine (TMPD) donates electrons to this low-O2 affinity pathway, causing reduction bleaching of a flavoprotein absorption band at 455 nm. Ascorbate-TMPD dependent respiration was partially (25%) inhibited by 200 μM quinacrine. The low O2-affinity oxidase activity promoted by NADH, or ascorbate plus TMPD was present in aerobic and microaerophilic grown cells and absent in anaerobic and bacteroid cells. Thus, a NADH linked flavoprotein type oxidase is suggested.
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Barquera, B., Garcia-Horsman, A. & Escamillá, J.E. An alternative non-cytochrome containing branch in the respiratory system of free-living Rhizobium phaseoli . Arch. Microbiol. 155, 428–435 (1991). https://doi.org/10.1007/BF00244957
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DOI: https://doi.org/10.1007/BF00244957