Abstract
The periplasmic reduction of the electron acceptors nitrate (Em +420 mV) and trimethylamine-N-oxide (TMAO; Em +130 mV) by Nap and Tor reductases is widespread in Gram-negative bacteria and is usually considered to be driven by non-energy conserving quinol dehydrogenases. The Epsilonproteobacterium Campylobacter jejuni can grow by nitrate and TMAO respiration and it has previously been assumed that these alternative pathways of electron transport are independent of the proton-motive menaquinol-cytochrome c reductase complex (QcrABC) that functions in oxygen-linked respiration. Here, we show that a qcrABC deletion mutant is completely deficient in oxygen-limited growth on both nitrate and TMAO and is unable to reduce these oxidants with physiological electron donors. As expected, the mutant grows normally on fumarate under oxygen-limited conditions. Thus, the periplasmic Nap and Tor reductases receive their electrons via QcrABC in C. jejuni, explaining the general absence of NapC and TorC quinol dehydrogenases in Epsilonproteobacteria. Moreover, the specific use of menaquinol (Em −75 mV) coupled with a Qcr complex to drive reduction of nitrate or TMAO against the proton-motive force allows the process to be electrogenic with a H+/2e− ratio of 2. The results have general implications for the role of Qcr complexes in bacterial oxygen-independent respiration and growth.
Similar content being viewed by others
Introduction
The cytochrome bc1 complex (referred to as Complex III in eukaryotic mitochondria) is a highly conserved proton-translocating, quinol-cytochrome c reductase (Qcr) that has a major role in oxygen-linked respiration in phylogenetically diverse prokaryotes1. The core of the complex consists of the membrane bound Rieske Fe-S protein and a diheam b containing cytochrome, combined with a membrane anchored c-type cytochrome facing the extracytoplasmic side of the cytoplasmic membrane. The complex functions via an electron bifurcating proton-motive Q-cycle that couples electron transfer from the quinol pool to periplasmic electron acceptors with proton translocation across the cytoplasmic membrane2. In one cycle, for every two electrons transferred, four protons are released to the extracytoplasmic side (p-phase) of the membrane and two protons are taken up from the cytoplasmic side (n-phase). The mechanistically similar cytochrome b6 f complex operates in chloroplasts and cyanobacteria, and connects the two photosystems of oxygenic photosynthesis1.
In mitochondria, and most lineages of Gram-negative bacteria where it is present, including the Alpha- Beta- and Gammaproteobacteria, the c-type cytochrome associated with the Qcr complex is a monohaem protein in the c1 family, that donates electrons to small soluble periplasmic c-type cytochromes which act to shuttle electrons to the terminal cytochrome c oxidase. However, uniquely in Epsilonproteobacteria, including the pathogens Campylobacter jejuni and Helicobacter pylori and the rumen bacterium Wolinella succinogenes, this cytochrome is a dihaem protein, a member of the cytochrome c4 family3. Phylogenetic and sequence/structure analysis suggests a mutational induced collapse of the dihaem structure during evolution has resulted in the cytochrome c1 type of molecule3. High GC Gram-positive bacteria also have a diheam cytochrome c associated with the Rieske/cytochrome b core. In these latter bacteria, soluble c-type cytochromes are absent; instead the oxidase interacts directly to form a “super-complex” that couples quinol oxidation with oxygen reduction4,5,6.
There are a few important examples where, in addition to its role in cytochrome c oxidase linked oxygen respiration, the bc1 complex can mediate electron transport to periplasmic reductases. The best studied of these are in denitrifying bacteria, such as Paracoccus denirificans, where the nitrous oxide reductase (Nos), nitric oxide reductase (Nor) and the copper- or cd1-type of nitrite reductases all receive their electrons by a cytochrome bc1 dependent route, via either cytochrome c or a small copper protein acting as an electron shuttle7,8,9. In addition, many Gram-negative bacteria have a periplasmic cytochrome c peroxidase that is commonly dependent on the cytochrome bc1 complex10. Genomic studies have revealed that some bacteria have genes for multiple separate Qcr type complexes, although their physiological roles are largely unknown11.
The presence of a Rieske/cytochrome b core complex containing the atypical diheam cytochrome c4 in Epsilonproteobacteria3 is of interest in relation to possible alternative functions of this complex in this group of bacteria. Recently, it was shown that in W. succinogenes, nitrate respiration via the periplasmic Nap reductase was unexpectedly severely inhibited in a mutant missing the Qcr complex12. This conflicts with the current model of nitrate reduction by the active site subunit NapA, which is based on electron transfer from menaquinol through the membrane associated NapGH subunits acting as a quinol dehydrogenase13,14.
In this study, we sought to clarify the role of the Qcr complex in the important Epsilonproteobacterium C. jejuni, which is the commonest cause of bacterial food-borne gastroenteritis in many countries15. This bacterium has a microaerophilic lifestyle and colonises the caeca of chickens to high levels; it infects humans mainly through consumption of undercooked poultry16. In addition to the use of oxygen as a preferred electron acceptor, various strains of C. jejuni can reduce fumarate, nitrate, nitrite, TMAO/DMSO, tetrathionate and hydrogen peroxide and many of these can support growth under severely oxygen-limited conditions17,18,19,20. Whether completely anaerobic growth occurs is controversial17,21 but due to the presence of a single oxygen-requiring ribonucleotide reductase essential for DNA synthesis17, a small amount of oxygen seems necessary for viability.
Although the assembly, composition and functions of the electron transport chains of C. jejuni have been clarified in recent years22,23,24, it has been assumed, based on models developed in other bacteria, that the major function of the proton-translocating Qcr complex is in oxygen-linked respiration. Here, we provide evidence from mutant studies for a hitherto unappreciated role for QcrABC in both nitrate and TMAO respiration. The results provide a rationale for the puzzling absence of the membrane bound quinol dehydrogenases NapC and TorC, that are essential for periplasmic nitrate and TMAO reduction respectively, in many other bacteria (e.g. E. coli), that lack a Qcr complex. Moreover, we show that by the use of menaquinol (Em −75 mV) as the electron donor to Qcr, the periplasmic reduction of nitrate, TMAO and certain other electron acceptors e.g. tetrathionate, can be an electrogenic process.
Results
Isolation and characterisation of a qcrABC deletion mutant
The genes cj1186c-cj1184c are operonic and encode the Rieske FeS subunit, the diheam cytochrome b subunit and the dihaem cytochrome c subunit, respectively, of the Qcr complex in C. jejuni (Fig. 1A). Although currently annotated as petABC (due to their homology with the Rhodobacter photosynthetic electron transport genes encoding a typical cytochrome bc1 complex), we propose that the qcrABC designation be used, as this more accurately reflects their function in this non-photosynthetic bacterium. QcrA and QcrB are similar to many other related homologues, with one transmembrane helix and nine transmembrane helices respectively, while QcrC is predicted to have two transmembrane helices and is significantly larger (41.4 kDa) than the W. succinogenes homologue (31.7 kDa).
Construction and verification of a qcrABC deletion mutant. (A) Mutagenesis strategy. The majority of the coding regions of the qcrA-C genes were replaced with a kanamycin resistance cassette with its own promoter. The downstream cfa gene has its own promoter, but the cassette has no terminator and so should be non-polar on cfa. (B) RT-PCR to verify expression of cfa gene and absence of qcr gene transcription in the qcrABC mutant. Fold-change in the mutant is shown relative to the wild type strain, using gyrA gene as a control. The difference between expression of the cfa gene in mutant and wild-type was not significant by t-test. (C) shows a comparison of the membrane associated c-type cytochromes revealed after a total membrane preparation was subjected to SDS-PAGE and electroblotting to a nitrocellulose membrane, followed by staining for haem-associated peroxidase activity using the chemiluminescence technique described in Methods. 20 μg total protein was run per lane. The Image shown is a cropped version of the full-size blot that can viewed in Supplementary Fig. 1, and was obtained using a ChemiDoc XRS system (BioRad Inc) with an exposure time of 2 min. A band corresponding to the expected size of QcrC is missing in the qcrABC deletion mutant.
The qcrABC genes were deleted from the chromosome of C. jejuni NCTC 11168 using allelic exchange via a plasmid containing upstream and downstream flanking regions for recombination and where the coding regions were replaced with a non-polar kanamycin resistance cassette with an outward reading promoter25 (Fig. 1A). Small colonies that developed on selective plates were shown to have the correct genotype by PCR with flanking primers (Table 1). Despite repeated attempts, we were unable to obtain a complemented strain with the wild-type qcrABC genes integrated at a distal locus. However, RT-PCR showed that transcription of the qcrA, qcrB and qcrC genes was absent in the mutant, and that the cfa gene, immediately downstream of the qcrABC operon, was not significantly altered in expression in the mutant compared to wild-type (Fig. 1B). To further confirm the phenotype of the qcrABC mutant, total membranes were prepared by differential centrifugation and subjected to haem blotting as described in Experimental Procedures, to specifically detect c-type cytochromes. Figure 1C shows a band of ~42 kDa consistent with size of QcrC (41.4 kDa) is present in wild-type membranes but missing in the qcrABC strain (the full size blot can be viewed in Supplementary Fig. 1).
Comparison of oxygen respiration and microaerobic growth phenotypes of qcrABC and ccoNOQP mutants: Deletion of qcrABC causes accumulation of ROS
Cells of WT, qcrABC and a ccoNOQP deletion strain24, were grown in complex media under standard microaerobic conditions and the specific rate of oxygen consumption in cell suspensions compared, with formate as electron donor. Figure 2A shows that the qcrABC and ccoNOQP strains had similar rates of formate-linked oxygen respiration but these were 58% and 68% lower respectively compared to the wild-type parent. These results are consistent with electron flux proceeding through the Qcr complex to CcoNOQP, thus giving a similar rate when the cognate genes of either complex are deleted, with the remaining rate being due to oxygen reduction by the alternative Qcr-independent CioAB menaquinol oxidase.
Phenotypic analysis of a qcrABC deletion mutant grown under microaerobic conditions. (A) shows the specific rate of formate dependent oxygen consumption measured by an oxygen electrode in cell suspensions of the strains indicated. About 68% of the electron flux to oxygen proceeds through the cytochrome c oxidase (CcoNOQP) and, as expected, this is similar in the qcrABC mutant, which is the source of electrons for CcoNOQP. The alternative oxidase (CioAB) accounts for the remaining electron flux. The data shown are means and standard deviation of triplicate determinations (*P < 0.05 by one way ANOVA. NS, not significant). (B) Growth curves of the strains indicated under microaerobic conditions. The qcrABC strain shows a larger reduction in growth rate and cell yield compared to the ccoNOQP mutant. The data shown are the means and standard deviations of triplicate growth curves; in most cases the error bars are too small to be seen. (C) Accumulation of ROS in microaerobically incubated cells suspensions of wild-type and qcrABC mutant strains. The fluorescence emission of 2′,7′ dihydrodichlorofluorescein diacetate (H2DCFDA) added to 10 μM final concentration is shown, normalized to total cell protein. Data are means and standard deviations of triplicate experiments. (**P < 0.01, ***P = 0.001 by multiple t-tests).
Figure 2B shows growth curves for wild-type, qcrABC and ccoNOQP strains under microaerobic conditions in complex media with no added exogenous electron acceptors. It is clear that the qcrABC strain has a severe growth defect, with a low final cell yield and doubling time of ~6 h in exponential phase compared to ~3.5 h for the wild-type. Interestingly, this is much slower than the ccoNOQP deletion mutant (~4.5 h doubling time), which receives electrons from the Qcr complex. We hypothesised that major disruption to the electron transport chain by removal of the Qcr complex might cause an accumulation of reactive oxygen species (ROS), particularly because the two periplasmic cytochrome c peroxidases in C. jejuni (Cj0020 and Cj0358) are thought to be dependent on the Qcr complex10,22. This was tested using the fluorescent ROS sensitive dye 2′,7′ dihydrodichlorofluorescein diacetate (H2DCFDA). We found much higher levels of ROS production in the qcrABC strain compared to wild-type when cells were resuspended and incubated in oxygenated buffer (Fig. 2C). Our previous studies using the same method have shown that the same ccoNOQP deletion mutant used here does not accumulate ROS above WT levels under these conditions24.
Nitrate and TMAO dependent oxygen-limited growth and respiration requires the QcrABC complex
Under oxygen-limited conditions in static broth cultures, we have previously shown that growth of C. jejuni NCTC 11168 is dependent on the addition of a range of alternative electron acceptors including fumarate (Em +30 mV), nitrate (Em +420 mV) or TMAO (Em +130 mV)17,18, although growth yields under these conditions are poor compared to microaerobic growth. There are two fumarate reductases present in C. jejuni, a menaquinol:fumarate reductase (FrdABC complex) and a methylmenaquinol:fumarate reductase (MfrABE complex) and thus electrons pass directly from the (methyl)menaquinol pool to fumarate via these enzymes19,26. Figure 3A shows that growth of the qcrABC mutant under oxygen-limited conditions with fumarate is identical to that of the parental wild-type strain and that neither strain grows in the absence of fumarate, showing that as expected, fumarate reduction is not dependent on the Qcr complex. This is an important control and the similar growth rate of both mutant and wild-type strains under these conditions, in contrast to the marked aerobic growth defect shown in Fig. 2B, further supports the view that the qcrABC mutant experiences significant oxidative stress in the presence of sufficient oxygen.
Comparison of growth of wild-type and qcrABC strains on fumarate and nitrate under oxygen-limited conditions. (A) growth of wild-type (red lines and symbols) and qcrABC mutant (blue lines and symbols) in the absence (open symbols) or presence (closed symbols) of 20 mM sodium fumarate. The mutant does not have a growth defect on fumarate, consistent with the two fumarate reductases present in C. jejuni deriving electrons directly from the (methyl)menaquinol pool. (B) growth of wild-type (red line and closed symbols) and qcrABC mutant (green line and closed symbols) in the presence of 20 mM sodium nitrate. (C) nitrite concentrations were measured in samples taken from the same growth curve as in (B) as described in Experimental Procedures. The data shown are single representative growth curves from several that have been performed with independent inocula. In each case similar results were obtained.
However, similar experiments with nitrate (Fig. 3B) or TMAO (Fig. 4A) as exogenous electron acceptors show that, in contrast to the wild-type, the qcrABC mutant is unable to grow with either of these oxidants. Nitrite accumulation from nitrate (Fig. 3C) and TMA accumulation from TMAO (Fig. 4B,C) only occur to any significant extent in wild-type cells and is closely correlated with growth. Taken together, the data indicate that the Qcr complex is required for both nitrate and TMAO-dependent growth and respiration. TMAO reduction was quantified as TMA accumulation at a chemical shift of 2.88–2.89 ppm using 1H-NMR spectroscopy of culture supernatants (Fig. 4B,C). During these experiments, we noted a resonance at 2.39 ppm increasing in intensity in the mutant cell supernatants, but much less in the wild-type (Fig. 4D). This peak has a natural abundance 13C resonance at 36.8 ppm, which matches succinate; it accumulated to ~1.2 mM after 7 h in the mutant, compared to ~ 0.5 mM in the wild-type. A peak at 2.36 ppm, increased in both WT and mutant (Fig. 4D) and had a natural abundance 13C peak at 29.2 ppm, matching pyruvate (~ 2 mM in both strains after 7 h).
Growth of wild-type and qcrABC strains on TMAO under oxygen-limited conditions and measurement of TMA accumulation. (A) growth of wild-type (black line and closed symbols) and qcrABC mutant (blue line and closed symbols) in the presence of 20 mM TMAO. Representative growth curve of several performed which all gave similar results. (B) 1H-NMR measurement of TMA accumulation during the growth curve shown in panel A. The region of chemical shift between 2.85 and 2.92 ppm is shown, with the TMA resonance at 2.885 ppm used for quantification. The different coloured spectra are media supernatants from samples taken every hour from time 0 (black spectra) to 7 h (yellow spectra), showing progressive TMA accumulation in the wild-type cells (black arrow) but no significant accumulation in the qcrABC mutant. The actual TMA concentrations are plotted in panel (C). Panel (D) shows the region of chemical shift between 2.32 and 2.40 ppm from the same spectra as in panel B, to illustrate the excretion of succinate (black arrow) in the qcrABC mutant.
The soluble c-type cytochromes CccB (Cj1020) and CccC (Cj0037) are not required for nitrate or TMAO reduction
Electron transfer from the Qcr complex to the Nap or Tor enzymes in the periplasm could occur either directly from QcrC, for example, or via additional periplasmic cytochromes. In C. jejuni, we previously identified and characterised three major periplasmic c-type cytochromes; two monohaem proteins, CccA and CccB, and the dihaem CccC24. Deletion mutants in cccB or cccC show only the loss of the cognate cytochromes, but deletion of cccA leads to an unusual pleiotropic phenotype involving the loss of all detectable periplasmic c-type cytochromes24. Therefore, we could not determine if electron transport to nitrate or TMAO requires CccA by mutant phenotypic studies. However, as Fig. 5 shows, a double cccB cccC mutant grows on fumarate, nitrate and TMAO under oxygen-limited conditions just as well as the wild-type, showing that neither of the soluble c-type cytochromes CccB or CccC are involved in electron transport to any of these electron acceptors.
The c-type cytochromes CccB (Cj1020) and CccC (Cj0037) are not required for oxygen-limited growth on fumarate, nitrate or TMAO. Panels (A–C) show representative growth curves of wild-type and the cccB cccC double mutant in static MHS media alone (open symbols) or in the presence of 20 mM sodium fumarate (closed symbols in A), 20 mM sodium nitrate (closed symbols in B) or 20 mM TMAO (closed symbols in C). The open symbol data are controls for the electron acceptor dependent growth and are the same in each panel.
Discussion
Until very recently, examples of the role of bacterial Qcr complexes in electron transport to a wider range of oxidants than just molecular oxygen was limited to hydrogen peroxide and the reduction of certain nitrogen oxides in denitrifying bacteria. The results presented here clearly show that nitrate and TMAO reduction and growth depend on electron transport through the Qcr complex in C. jejuni. Our data support recent experiments in the related Epsilonproteobacterium W. succinogenes, which also unexpectedly showed a dependency of nitrate reduction on the Qcr complex12. Both bacteria possess a single periplasmic nitrate reductase of the Nap type, with the catalytic NapA subunit receiving electrons via a diheam cytochrome c subunit, NapB (Fig. 6). The periplasmic Nap class of nitrate reductases are very widespread in many phylogenetically diverse groups of bacteria and have been thought to be obligately coupled to quinol oxidation through the intermediacy of either the NapC class of tetrahaem quinol dehydrogenases or, in bacteria like C. jejuni and W. succinogenes, where NapC is absent, the NapGH proteins (Fig. 6), which have been proposed to form an alternative quinol dehydrogenase module13,14,27. In either case, because quinol oxidation (and thus proton release) occurs on the periplasmic side of the membrane, into the same cellular compartment that nitrate reduction (and thus proton consumption) occurs, electron transfer from quinol to nitrate by this mechanism is not energy conserving14,28.
Model for electrogenic nitrate reduction in C. jejuni. The redox potential span between the MK/MKH2 and the nitrate/nitrite couple is large enough to allow for a Qcr dependent electrogenic mechanism, given a typical transmembrane Δp of ~180 mV. The operation of the Qcr complex directly coupled to the NapAB enzyme gives a net transmembrane proton translocation of 2 H+ per 2e− transferred from menaquinol. There is uncertainty about the precise route of electron transfer from the Qcr complex to NapB (dashed arrows) but the simplest mechanism would be direct transfer from haem 2 of QcrC to haem 1 of NapB. The role of NapG and NapH, which are known to be essential for nitrate respiration, is unclear. NapG may act in the electron transfer pathway itself or NapGH may act as a quinol dehydrogenase involved in a reductive maturation process e.g. for NapA. Black filled circles in QcrC and NapB represent the haems.
The midpoint redox potential of the nitrate/nitrite couple is highly positive (Em +420 mV) and in principle nitrate reduction could be driven by either ubiquinol oxidation (Em +90 mV) with a ΔE of 330 mV, or menaquinol oxidation (Em −75 mV) with a ΔE of 495 mV. Indeed, in E. coli, it is thought that NapC catalyses electron transfer from menaquinol to NapB, whereas NapGH is more specific for ubiquinol29. For a proton-motive Qcr-dependent mechanism of nitrate reduction, however, the redox potential of the quinol used becomes more critical as a pre-existing proton-motive force of ~180 mV, positive outside, exists across the cytoplasmic membrane, against which the Q-cycle mechanism has to operate. C. jejuni synthesises menaquinones but not ubiquinone, and so employs the energetically more favourable menaquinol dependent reduction of nitrate which, via the Qcr mechanism, would be electrogenic with a net H+/2e ratio of 2 (Fig. 6). Note that recent measurements of Δp in C. jejuni, using a permeant cation redistribution method, gave values of ~100–120 mV30, but this is an underestimate of the actual value expected for an energy transducing membrane.
Our results, and those of Hein et al.12 provide a rationale for the absence of NapC in Epsilonproteobacteria, but call into question the proposed role of NapGH as a quinol dehydrogenase in these and other bacteria employing a Qcr-complex for nitrate reduction. Nevertheless, from previous mutant studies in W. succinogenes31 it has been established that NapG and NapH are essential for NapA dependent nitrate reduction. In C. jejuni napH is also essential for nitrate respiration and deletion of napG is highly deleterious18, with residual growth thought to be due to the ability of the quinol dehydrogenase of the nitrite reductase (NrfH) to donate electrons to NapB18. NapG and NapH are possibly involved after electrons leave the Qcr complex, but the route by which electrons might be transferred to them requires investigation. NapG is membrane bound but facing the periplasm, with several FeS redox centres and so direct transfer from the quinol oxidase site in QcrB or the periplasmically located c-haems in QcrC are both possible. However, in the light of our current results, we think it more likely that QcrC can donate electrons directly to NapB and that NapGH has some other role (Fig. 6), for example in the reductive maturation of NapA, as has been discussed previously32. Irrespective of their precise function in the process, the key bioenergetic consequence of nitrate reduction being dependent on the Qcr complex is that electron transfer from menaquinol to nitrate will be energy conserving. It should be noted that in addition to the Nap-type of nitrate reductase, evidence is also emerging for a role for the Qcr complex in nitrate reduction in Streptomyces, which uses the membrane bound Nar-type of enzyme33, possibly suggesting direct Qcr-Nar electron transfer in this case.
That TMAO reduction is also Qcr dependent in C. jejuni now explains the long-standing puzzling observation that there is no homologue of the membrane bound pentahaem quinol dehydrogenase TorC in this bacterium17,22, and it has hitherto been unclear how quinol oxidation is coupled to TMAO reduction. TorC has been characterised best in E. coli34 and is a member of the widespread NapC /NrfH family, which are commonly tetrahaem proteins. It consists of an N-terminal membrane anchored domain, a central periplasmic domain containing the four core haem c centres and an additional C-terminal periplasmic domain containing a fifth c-type haem (Supplementary Fig. 2 and Fig. 7A). Electron transfer from TorC to the catalytic molybdoenzyme subunit TorA occurs via this fifth haem in the C-terminal domain34 (Fig. 7A). Interestingly, in C. jejuni, TorA (Cj0264) is associated with a small soluble monohaem c-type cytochrome (TorB; Cj0265), which is absent from E. coli, but which is homologous to the C-terminal domain of TorC (see the alignment in Supplementary Fig. 2 and Fig. 7B). Thus, we suggest that the functions of TorB and the C-terminal domain of TorC are equivalent and that this domain has been retained as a soluble protein in bacteria like C. jejuni that use a Qcr-dependent mechanism of TMAO reduction, presumably to specifically receive electrons from QcrC.
Comparison of the mechanism of TMAO reduction in E. coli and C. jejuni. (A) shows how quinol oxidation on the periplasmic side of the membrane in E. coli is coupled to electron transfer (black arrows) through the five haems in TorC (black filled circles) and the [4Fe-4S] and molybdopterin guanine dinucleotide (MGD) cofactors in TorA, ultimately reducing TMAO to TMA. Note that this mechanism is not energy-conserving (H+/2e− transferred is 0) because proton release from quinol oxidation and proton uptake during TMAO reduction occur in the same compartment (the periplasm). (B) shows the proposed energy-conserving mechanism of TMAO reduction in C. jejuni coupled to the proton-motive Q cycle in the Qcr complex, which gives an overall net H+/2e− ratio of 2. Although the redox span between menaquinol and TMAO is 205 mV, close to the typical value of ~180 mV for Δp, continued diffusion of TMA away from the system (dashed black arrow) would shift the equilibrium and favour electrogenic TMAO reduction. TorB (Cj0265), homologous to the C-terminal domain of TorC (see Supplementary Fig. 2), most likely receives electrons directly from QcrC (black arrows), before transfer to the redox centres in TorA. bL, low-potential haem b; bH, high-potential haem b in the QcrB subunit.
What are the bioenergetic implications of a Qcr-dependent mechanism of TMAO reduction? While it is clearly unfavourable thermodynamically for ubiquinol oxidation (Em +90 mV) to drive the reduction of TMAO (Em +130 mV) against a typical Δp across the membrane of ~180 mV, TMAO reduction by menaquinol (Em −75 mV) has a ΔE of 205 mV. Although this would seemingly be close to the limit of electrogenic function for a Qcr-dependent, Q-cycle mechanism across an energised membrane, the product of TMAO reduction is TMA, which is a water soluble gas which would tend to diffuse out of the system. This could help shift the equilibrium and provide a thermodynamic pull that would effectively increase the ΔE available to drive the reaction. It should be noted that C. jejuni also synthesises methylmenaquinone (MMK) with an Em of −125 mV for the MMK/MMKH2 couple; if MMKH2 could be used to drive TMAO reduction then the ΔE increases to 255 mV. Thus, the model of TMAO reduction in C. jejuni we now propose (Fig. 7B) is, like nitrate reduction, electrogenic with a net H+/2e− ratio from menaquinol oxidation of 2, whereas in E. coli it is 0, because TorC is a non proton-motive menaquinol dehydrogenase releasing protons into the periplasm (Fig. 7A); in the latter case, it is only at the level of the primary dehydrogenases that energy can be conserved during TMAO (or indeed Nap dependent nitrate) reduction.
We noted that ~2-fold more succinate was excreted during oxygen-limited growth of the qcrABC mutant on TMAO, which might be a compensatory mechanism to maintain redox balance by disposing of electrons through a Qcr independent route, i.e. fumarate reduction, although the low rate did not allow any significant growth. Both wild-type and mutant excreted some pyruvate under these oxygen-limited conditions, and this has been noted previously in C. jejuni grown under very low oxygen availability35.
Although we have focussed on the clear phenotypes of the qcrABC mutant in relation to nitrate and TMAO reduction under oxygen-limited growth conditions, we also noted that this mutant grew far more poorly with oxygen as electron acceptor compared to a mutant deficient in the cytochrome c oxidase, CcoNOQP. We hypothesised that a growth inhibiting build-up of ROS might result from removal of the Qcr complex, because of its role as electron donor to the two periplasmic cytochrome c peroxidases (Cj0020 and Cj0358). Measurements using a ROS sensitive dye suggested a much higher accumulation of ROS was indeed occurring in the qcr mutant compared to the wild-type. There may also be other reasons why ROS would accumulate in this mutant, for example if there is a reduction in its ability to properly oxidise the quinol pool and electrons are transferred to oxygen by non-physiological routes.
These new results concerning the reduction of nitrate and TMAO in C. jejuni not only change our overall view of the bioenergetics of this pathogen but, more generally, help to rationalise the use of menaquinol in electrogenic Qcr-dependent electron transport pathways to alternative acceptors of moderate redox potential. For example, the recent discovery of the TsdA type of bidirectional tetrathionate reductase in some strains of C. jejuni and many other bacteria20, which is a soluble periplasmic diheam cytochrome c, also suggests the involvement of the Qcr complex in this mode of tetrathionate reduction. The recent experimental determination of the midpoint redox potential of the tetrathionate/thiosulphate couple as +198 mV36 rather than the previously assumed value of +24 mV, means that the redox span using menaquinol to reduce tetrathionate is 273 mV, allowing the Qcr complex to operate electrogenically against the Δp, as with TMAO and nitrate.
Nitrate, TMAO and tetrathionate are present in the mammalian host intestinal environment and there is evidence that Salmonella uses nitrate reduction via the Nap system37 and tetrathionate reduction (in this case via the molybdoenzyme Tttr38) to gain a competitive advantage in host infection. While torA deletion mutants of C. jejuni do not show gross colonisation defects in a chicken model of colonisation, a napA mutant colonised at lower levels than the wild-type21. Given the conservation of Qcr-dependent pathways to electron acceptors other than oxygen in many C. jejuni strains, we suggest they are important in the infection biology of this pathogen.
Experimental Procedures
Bacterial strains, media and general culture conditions
Campylobacter jejuni strain NCTC 11168 was the wild-type strain used in this study. An isogenic ccoNOQP deletion mutant was previously constructed and described24. Individual deletion mutants in cccB (cj1020c::cat) and cccC (cj0037::kan) have also been previously described and characterised24; a double mutant was made by transformation of the deletion mutant plasmid pGEM1020CAT24 into the cccC mutant. Construction of the qcrABC deletion mutant is described below. All strains were routinely grown on Columbia Blood Agar base CM0331 (Oxoid, Basingstoke, UK) containing 5% (v/v) lysed horse blood (SR0050C, Thermo Scientific) and 10 μg/ml each of amphotericin B and vancomycin (referred to as CBA media) at 42 °C under microaerobic conditions [10% (v/v) O2, 5% (v/v) CO2 and 85% (v/v) N2] in a MACS growth cabinet (Don Whitley Scientific, Shipley, UK). Selective antibiotics (either kanamycin or chloramphenicol) were added where appropriate to a final concentration of 50 μg ml−1. From plates, bacterial cells were inoculated in 50–100 ml Mueller-Hinton broth (Oxoid) plus 20mM L-Serine (MHS) in 250 ml conical flasks. Liquid cultures for microaerobic growth were shaken at 200 rpm on a KS125 IKA-labortechnic shaker (IKA, Staufen, Germany) at 42 °C in the MACS growth cabinet as above. For oxygen-limited growth, cells were grown in 200 ml MHS in 250 ml conical flasks in the MACS growth cabinet as above, but without shaking. Where required, the electron acceptors sodium fumarate, sodium nitrate or TMAO were added to a final concentration of 20 mM from filter-sterilised stocks.
DNA manipulation and construction of a qcrABC deletion mutant
Chromosomal DNA of C. jejuni was extracted by using the wizard genomic DNA purification kit (Promega). Standard techniques were employed for the cloning, transformation, preparation and restriction analysis of plasmid DNA from E. coli39. A plasmid vector (pGEMQCR) for allelic exchange mutagenesis of qcrABC was assembled using pGEM3zf (Promega), 500 bp upstream and downstream fragments flanking qcrABC and a kanamycin resistance cassette derived from pJMK3025 using the Gibson isothermal assembly method, as previously described24. The two flanking fragments were PCR amplified using primers qcr_ISA_F1F/F1R and qcr_ISA_F2F/F2R (Table 1) with adapters homologous to 30 bp around the HincII site of the pGEM3zf multiple cloning site and 30 bp at the start or end of the kanamycin resistance cassette, which was amplified using primers Kan_F and Kan R (Table 1). An isothermal assembly reaction was carried out at 50 °C for 1 h with equimolar amounts of both flanking fragments, kanamycin cassette and HincII digested pGEM3zf in a reaction master mix containing isothermal assembly buffer, T5 Exonuclease, Phusion polymerase and Taq ligase24. The mixture was then transformed directly into competent E. coli DH5a cells with selection for kanamycin resistance. Plasmid pGEMQCR was checked by automated DNA sequencing using standard vector M13 primers. C. jejuni cells were grown in MHS overnight, pelleted and washed in 1 ml of ice cold wash buffer (9% (w/v) sucrose and 15% (w/v) glycerol in water), 3–4 times and finally resuspended in 200–300 μl of ice cold wash buffer. pGEMQCR was electrotransformed into these cells (2.5 kV, 200 Ohms, 25 μF; Bio-Rad Gene Pulser), which were then spread onto non-selective CBA plates. After overnight incubation, cell growth was transferrred to CBA plates containing kanamycin. Colonies appeared within 2–4 days, which were PCR screened with primers qcr_screen_F and qcr_screen_R (Table 1) that anneal approximately 100 bp upstream and downstream of the qcr operon respectively.
RT-PCR
Aliquots (5 ml) of mid-log microaerobically grown cultures (OD600 nm ~0.6) were pelleted and washed in 1 ml 20 mM sodium phosphate buffer, pH 7.4, then 5 µl phenol and 50 µl ethanol mixed in before re-pelleting. Phenol-pellets were stored at −80 °C. RNA was purified from phenol-pellets using the TRIzol Max Bacterial RNA Isolation Kit (Thermo Fisher) and subsequently DNase treated using the TURBO DNase Kit (Invitrogen), following the manufacturers protocols. The absence of contaminating DNA was confirmed by PCR screening using MyTaq Red Mix (Bioline). RT-PCR was performed using the SensiFAST SYBR kit (Bioline), with 20 µl reactions contained in 96-well plates following the manufacturers recommendations. Genomic DNA serial dilution controls were performed for each primer set to generate a standard CT curve. The RT-PCR was performed in an Mx3005P cycler, controlled by MxPro software (Stratagene). CT values generated by the software were manually processed to generate fold RNA changes, relative to wildtype, using gyrA as the housekeeping control gene. DNA controls were performed in duplicate, while RNA screening reactions were performed in quadruplicate. Primers used for RT-PCR are listed in Table 1.
Microaerobic and oxygen-limited growth curves, and determination of nitrite
For comparisons of microaerobic growth, cells from overnight starter cultures in the MACS cabinet were inoculated in 200 ml MHS in 500 ml conical flasks with appropriate antibiotics to a final OD (600 nm) of 0.1 and grown at 42 °C until an OD 600 nm of approximately 0.4–0.5. They were then back diluted to an OD 600 nm of 0.1 in fresh 100 ml MHS in shaken 250 ml conical flasks and the OD 600 nm monitored every hour using Jenway 6705 UV spectrophotometer. All Growth curves were done in triplicates. For growth and supernatant sample collection under oxygen-limited conditions, cells grown microaerobically as above, were back diluted to an OD of 0.1 in fresh 200 ml MHS in 250 ml conical flasks, with and without TMAO, nitrate or fumarate, and OD 600 nm monitored every hour. Cells in 1 ml samples were separated from the media supernatant by centrifugation (13,800 × g, 5 min) and the supernatants removed and stored frozen at −20 °C until ready for analysis. For nitrite determination, diluted culture supernatants (50 μl) from oxygen-limited growth experiments were added to 850 μl of 1% (w/v) sulphanilamide (Sigma) dissolved in 1 M HCl and 100 μl of 0.02% (w/v) naphthylethylenediamine (Sigma). After 15 min, the absorbance at 540 nm was measured and nitrite concentrations were determined by reference to a standard curve.
1H and 13C-NMR
For analysis of TMAO reduction, trimethylamine (TMA) concentrations in supernatant samples were quantified by 1H-NMR. To 800 μl of sample 10 μl of 100 mM trimethylsilyl propionate (TSP) was added as a 0 ppm chemical shift and quantitation reference. 450 μl of the mixture was transferred to NMR tubes, 50 μl of D2O was added and spectra acquired as described by Sellars et al.17. Integration of the single peak of TMA at 2.88–2.89 ppm and comparison with the TSP peak allowed TMA concentrations to be calculated. The 13C chemical shifts of succinate and pyruvate were obtained from a 2D 1H−13C HSQC spectrum, acquired with the standard Bruker pulse program, hsqcetgpsisp2, on an 800 MHz Avance I NMR spectrometer. The data were acquired with 2048 points in the direct dimension and 300 complex points in the indirect dimension, 64 transients per indirect point and a SW of 100 ppm for 13C.
Total membrane protein isolation and detection of c-type cytochromes
Cell cultures were grown overnight in 500 ml MHS, harvested by centrifuging at 8,000 × g, 4 °C for 20 min and resuspended in 5 ml of 10 mM HEPES buffer (pH 7.4). Cells were broken by sonication for 6 × 20 s at a frequency of 16 microns amplitude (MSE sonicator). Unbroken cells and debris were removed by centrifuging at 15,000 × g, 4 °C for 30 min. The supernatant was then centrifuged at 100,000 × g, 4 °C for 1 h in a benchtop ultracentrifuge (Beckman). The supernatant was discarded and the membrane pellet was washed and resuspended in 1 ml 25 mM phosphate buffer (pH 7.4). Total protein concentration was determined by Lowry assay. Proteins were denatured gently by incubating for 1 h at 37 °C in SDS-PAGE sample buffer but without β-mercaptoethanol. Proteins were separated by SDS-PAGE on 10% acrylamide gels and either stained with Coomassie blue G250 or electroblotted onto nitrocellulose membrane (Hybond-C extra, GE Healthcare). Covalently bound haem was detected as haem-associated peroxidase activity40, using the enhanced chemiluminesence (ECL) kit from GE Healthcare. Images were obtained using a ChemiDoc XRS system (BioRad Inc) with an exposure time of 2 min.
Measurement of substrate respiration rate in intact cells
Respiration rates were measured as the rate of oxygen consumption of cell suspensions in a Clark-type oxygen electrode using 10 mM sodium formate as electron donor, calibrated using air-saturated 25 mM phosphate buffer (pH 7.4) (200 nmol dissolved O2 ml−1 at 42 °C). Total protein concentration of the cell suspension was determined by Lowry assay at 600 nm and the specific rate of oxidation was calculated as nmol oxygen produced min−1 mg−1 total protein.
Measurement of Reactive Oxygen Species (ROS)
Cells were grown microaerobically in MHS and harvested at mid-expoential growth phase by centrifugation (8,000 × g, for 3 min). Cell pellets were washed and resuspended in 5 ml of 25 mM phosphate buffer (pH 7.4). Cells were added to 6 ml of 25 mM phosphate buffer (pH 7.4) in 6-well plates to a final OD 600 nm of 0.2. 2′,7′ dihydrodichlorofluorescein diacetate (H2DCFDA; Life Technologies, USA), dissolved in 1% DMSO, was added to a final concentration of 10 μM at time zero and the plates incubated microaerobically at 42 °C. Samples (1 ml) were removed every 10 min and fluorescence emission at 538 nm measured on a Cary Eclipse (Agilent), fluorimeter, with excitation at 485 nm. Total protein concentration of the cell suspension was determined by Lowry assay and the data expressed as fluorescence intensity per mg protein.
Data Availability
All data generated or analysed during this study are included in this published article.
References
Dibrova, D. V., Cherepanov, D. A., Galperin, M. Y., Skulachev, V. P. & Mulkidjanian, A. Y. Evolution of cytochrome bc complexes: From membrane-anchored dehydrogenases of ancient bacteria to triggers of apoptosis in vertebrates. Biochim Biophys Acta 1827, 1407–1427 (2013).
Xia, D. et al. Structural analysis of cytochrome bc 1 complexes: implications to the mechanism of function. Biochim Biophys Acta 1827, 1278–1294 (2013).
Baymann, F., Lebrun, E. & Nitschke, W. Mitochondrial cytochrome c 1 is a collapsed di-heme cytochrome. Proc Natl Acad Sci USA 101, 17737–17740 (2004).
Niebisch, A. & Bott, M. Molecular analysis of the cytochrome bc 1 -aa 3 branch of the Corynebacterium glutamicum respiratory chain containing an unusual diheme cytochrome c 1. Arch Microbiol 174, 282–294 (2001).
Graf, S. et al. Rapid Electron Transfer within the III-IV Supercomplex in Corynebacterium glutamicum. Sci Rep. 6, 34098, https://doi.org/10.1038/srep34098 (2016).
Kao, W. C. et al. The obligate respiratory supercomplex from Actinobacteria. Biochim Biophys Acta. 1857, 1705–1714 (2016).
Alefounder, P. R. & Ferguson, S. J. Electron transport-linked nitrous oxide synthesis and reduction by Paracoccus denitrificans monitored with an electrode. Biochem Biophys Res Commun. 104, 1149–1155 (1982).
Carr, G. J., Page, M. D. & Ferguson, S. J. The energy-conserving nitric-oxide-reductase system in Paracoccus denitrificans. Distinction from the nitrite reductase that catalyses synthesis of nitric oxide and evidence from trapping experiments for nitric oxide as a free intermediate during denitrification. Eur J Biochem. 179, 683–692 (1989).
Pearson, I. V., Page, M. D., van Spanning, R. J. & Ferguson, S. J. A mutant of Paracoccus denitrificans with disrupted genes coding for cytochrome c 550 and pseudoazurin establishes these two proteins as the in vivo electron donors to cytochrome c d 1 nitrite reductase. J Bacteriol. 185, 6308–6315 (2003).
Atack, J. M. & Kelly, D. J. Structure, mechanism and physiological roles of bacterial cytochrome c peroxidases. Adv Microb Physiol. 52, 73–106 (2007).
ten Brink, F., Schoepp-Cothenet, B., van Lis, R., Nitschke, W. & Baymann, F. Multiple Rieske/cyt b complexes in a single organism. Biochim Biophys Acta. 1827, 1392–406 (2013).
Hein, S., Witt, S. & Simon, J. Clade II nitrous oxide respiration of Wolinella succinogenes depends on the NosG, -C 1, -C 2, -H electron transport module, NosB and a Rieske/cytochrome bc complex. Environ Microbiol. 19, 4913–4925 (2017).
Kern, M. & Simon, J. Characterization of the NapGH quinol dehydrogenase complex involved in Wolinella succinogenes nitrate respiration. Mol Microbiol. 69, 1137–1152 (2008).
Kern, M. & Simon, J. Electron transport chains and bioenergetics of respiratory nitrogen metabolism in Wolinella succinogenes and other Epsilonproteobacteria. Biochim Biophys Acta. 1787, 646–656 (2009).
O’Brien, S. J. & Sarah, J. The consequences of Campylobacter infection. Curr Opin Gastroenterol. 33, 14–20 (2017).
Sheppard, S. K. et al. Campylobacter genotyping to determine the source of human infection. Clin Infect Dis 48, 1072–1078 (2009).
Sellars, M. J., Hall, S. J. & Kelly, D. J. Growth of Campylobacter jejuni supported by respiration of fumarate, nitrate, nitrite, trimethylamine-N-oxide, or dimethyl sulfoxide requires oxygen. J Bacteriol. 184, 4187–4196 (2002).
Pittman, M. S. et al. Growth of Campylobacter jejuni on nitrate and nitrite: electron transport to NapA and NrfA via NrfH and distinct roles for NrfA and the globin Cgb in protection against nitrosative stress. Mol Microbiol. 63, 575–590 (2007).
Weingarten, R. A., Taveirne, M. E. & Olson, J. W. The dual-functioning fumarate reductase is the sole succinate: quinone reductase in Campylobacter jejuni and is required for full host colonization. J Bacteriol. 191, 5293–52300 (2009).
Liu, Y. W., Denkmann, K., Kosciow, K., Dahl, C. & Kelly, D. J. Tetrathionate stimulated growth of Campylobacter jejuni identifies a new type of bi-functional tetrathionate reductase (TsdA) that is widely distributed in bacteria. Mol Microbiol. 88, 173–188 (2013).
Weingarten, R. A., Grimes, J. L. & Olson, J. W. Role of Campylobacter jejuni respiratory oxidases and reductases in host colonization. Appl Environ Microbiol. 74, 1367–1375 (2008).
Myers, J. D. & Kelly, D. J. Respiratory electron transport in Helicobacter and Campylobacter, (ed. Zannoni, D.) 63–80 (Respiration in Archaea and Bacteria, 2004) Springer, Netherlands.
Kelly, D. Complexity and Versatility in the Physiology and Metabolism of Campylobacter jejuni, (ed. In Nachamkin, I., Szymanski, C. & Blaser, M.) 41–61 (Campylobacter, Third Edition. 2008) ASM Press, Washington, DC., https://doi.org/10.1128/9781555815554.ch3.
Liu, Y. W. & Kelly, D. J. Cytochrome c biogenesis in Campylobacter jejuni requires cytochrome c 6 (CccA; Cj1153) to maintain apocytochrome cysteine thiols in a reduced state for haem attachment. Mol Microbiol. 96, 1298–1317 (2015).
van Vliet, A. H., Wooldridge, K. G. & Ketley, J. M. Iron-responsive gene regulation in a Campylobacter jejuni fur mutant. J Bacteriol. 180, 5291–5298 (1998).
Guccione, E. et al. Reduction of fumarate, mesaconate and crotonate by Mfr, a novel oxygen-regulated periplasmic reductase in Campylobacter jejuni. Environ Microbiol. 12, 576–591 (2010).
Cole, J. A. & Richardson, D. J. Respiration of Nitrate and Nitrite. EcoSal Plus. 3, https://doi.org/10.1128/ecosal.3.2.5. (2008).
Simon, J., van Spanning, R. J. & Richardson, D. J. The organisation of proton motive and non-proton motive redox loops in prokaryotic respiratory systems. Biochim Biophys Acta. 1777, 1480–1490 (2008).
Brondijk, T. H., Nilavongse, A., Filenko, N., Richardson, D. J. & Cole, J. A. NapGH components of the periplasmic nitrate reductase of Escherichia coli K-12: location, topology and physiological roles in quinol oxidation and redox balancing. Biochem J. 379, 47–55 (2004).
van der Stel, A-X. et al. Generation of the membrane potential and its impact on the motility, ATP production and growth in Campylobacter jejuni. Mol Microbiol 105, 637–651 (2017).
Kern, M., Mager, A. M. & Simon, J. Role of individual nap gene cluster products in NapC-independent nitrate respiration of Wolinella succinogenes. Microbiology. 153, 3739–3747 (2007).
Kern, M. & Simon, J. Periplasmic nitrate reduction in Wolinella succinogenes: cytoplasmic NapF facilitates NapA maturation and requires the menaquinol dehydrogenase NapH for membrane attachment. Microbiology. 155, 2784–94 (2009).
Sawers, R. G., Falke, D. & Fischer, M. Oxygen and Nitrate Respiration in Streptomyces coelicolor A 3(2). Adv Microb Physiol. 68, 1–40 (2016).
Gon, S., Giudici-Orticoni, M. T., Méjean, V. & Iobbi-Nivol, C. Electron transfer and binding of the c-type cytochrome TorC to the trimethylamine N-oxide reductase in Escherichia coli. J Biol Chem. 276, 11545–11551 (2001).
Guccione, E. et al. Transcriptome and proteome dynamics in chemostat culture reveal how Campylobacter jejuni modulates metabolism, stress responses and virulence factors upon changes in oxygen availability. Environ Microbiol. 19, 4326–4348 (2017).
Kurth, J. M., Dahl, C. & Butt, J. N. Catalytic protein film electrochemistry provides a direct measure of the tetrathionate/thiosulfate reduction potential. J Am Chem Soc. 137, 13232–13235 (2015).
Lopez, C. A., Rivera-Chávez, F., Byndloss, M. X. & Bäumler, A. J. The Periplasmic Nitrate Reductase NapABC Supports Luminal Growth of Salmonella enterica Serovar Typhimurium during Colitis. Infect Immun. 83, 3470–3478 (2015).
Winter, S. E. et al. Gut inflammation provides a respiratory electron acceptor for Salmonella. Nature. 467, 426–429 (2010).
Sambrook, J., Fritsch, E. F. & Maniatis, T. Molecular cloning: a laboratory manual, (ed. Sambrook, J) 1–1546 (Molecular cloning: a laboratory manual, Second Edition, 1989) Cold Spring Harbor Laboratory Press, New York.
Feissner, R., Xiang, Y. & Kranz, R. G. Chemiluminescent-based methods to detect subpicomole levels of c-type cytochromes. Anal Biochem. 315, 90–94 (2003).
Acknowledgements
This work was funded by a University of Sheffield Vice Chancellors Indian Scholarship to NG and a UK Biotechnology and a Biological Sciences Research Council (BBSRC) grant (BB/R003491/1) to DJK for AJT. We thank Dr. Jörg Simon for insightful discussions on the role of the Qcr complex in epsilonproteobacteria. We acknowledge Yang Wei Liu for construction of the cccB cccC mutant and Mrs. Andrea Hounslow for performing the NMR in the departmental biomolecular NMR facility. This paper is dedicated to the memory of J. Baz Jackson, an inspirational mentor.
Author information
Authors and Affiliations
Contributions
N.G. and A.J.T. performed all experiments. D.J.K. wrote the manuscript and N.G., A.J.T. and D.J.K. edited and reviewed the text.
Corresponding author
Ethics declarations
Competing Interests
The authors declare no competing interests.
Additional information
Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Electronic supplementary material
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Garg, N., Taylor, A.J. & Kelly, D.J. Bacterial periplasmic nitrate and trimethylamine-N-oxide respiration coupled to menaquinol-cytochrome c reductase (Qcr): Implications for electrogenic reduction of alternative electron acceptors. Sci Rep 8, 15478 (2018). https://doi.org/10.1038/s41598-018-33857-2
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41598-018-33857-2
- Springer Nature Limited