Summary
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1.
By difference spectrophotometry the cytochrome pattern of two strains of Rhizobium leguminosarum — effective and partly-effective — was studied in pure culture and in bacteroids isolated from legume root nodulesFootnote 1.
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2.
In pure culture both strains contained cytochromes c, b and a-a 3 as well as cytochrome a 2 which appeared under semi-anaerobic conditions, especially in the effective strain. In Rhizobia cytochrome a 2 was detected for the first time.
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3.
Bacteroids of the effective strain contained cytochromes c, b and some CO-reactive pigments which remained incompletely identified. Bacteroids of the partly-effective strain contained cytochrome a (a = peak at 597 nm at − 196°) which was absent from bacteroids of the effective strain.
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4.
The data obtained show that the low O2 concentration within nodules does not seem to be the only factor responsible for the absence of cytochromes a from N2-fixing bacteroids.
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Notes
The term bacteroids is applied to all forms of the specific strain of bacteria isolated from root nodules.
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Kretovich, W.L., Romanov, V.I. & Korolyov, A.V. Rhizobium leguminosarum cytochromes (Vicia faba). Plant Soil 39, 619–634 (1973). https://doi.org/10.1007/BF00264179
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DOI: https://doi.org/10.1007/BF00264179