Summary
A shear-sensitive hybridoma cell line, incapable of growth or antibody production in spinner or shake flasks agitated at 40 rpm, was grown successfully in a perfusion propagation system consisting of a bioreactor (1.5 liter), stirred with a cell-lift impeller at 60 rpm, and a tangential flow filtration unit for removal of spent culture medium from the reactor. The culture was maintained over a 48 day period and cell numbers reached 1.8 × 107 cells/ml. Maximal monoclonal antibody concentration was 800 ug/ml, indicating a productivity of 504 mg/day.
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Adamson, S.R., S.L. Fitzpatrick, L.A. Behie, G.M. Gaucher and B.H. Lesser, 1983, Biotechnol. Lett. 5, 573.
Brown, P.C., M.A.C. Costello, R. Oakley and J.L. Lewis, 1985. In “Large Scale Mammalian Cell Culture” (J. Feder and W.R. Tolbert eds.) pp.59–71, Academic Press.
de St. Groth, F., 1983, J. Immuno. Methods 57, 121.
Hopkinson, J., 1985, Biotechnology 3, 225.
Littlefield, S.G., K.J. Gilligan and A.P. Jarvis, 1984, Hybridoma 3, 75.
Lydersen, B.K., J. Putnam, E. Bognar, M. Patterson, G.G. Pugh and L.A. Noll, 1985. In “Large Scale Mammalian Cell Culture” (J. Feder and W.R. Tolbert eds.) pp. 39–58, Academic Press.
Feder, J. and W.R. Tolbert, 1985, Am. Biotechnol. Lab. 3, 24.
Macmillan, J.D., Velez, D., Miller L. and Reuveny, S. 1986. Monoclonal antibody production in stirred reactors. In Lydersen, B.K. (ed) Large Scale Cell Culture Technology. Carl Hanser BmbH & Co., Munich. (In Press).
Meyer, R.F., Miller, L., Bennett, R.W. and Macmillan, J.D., 1984. Appl. Environ. Microbiol. 47, 283.
Patterson, Jr., M.K., 1979, Methods Enzymol. 58, 141.
Reuveny, S., Velez, D., Miller, L. and Macmillan, J.D., 1986a. J. Immunol. Methods 86, 61.
Reuveny, S., Zheng, Z. B. and Eppstein, L., 1986b. Am. Biotechnol. Lab. 1, 28.
Seaver, S., Rudolph J.L., Ducibella, T. and Gabriels, J.W., 1984. InThe World Biotech Report 1984, Vol. 2: U.S.A. (Online Publications, New York, NY) p. 325.
Thompson, N.E., Bergdoll, M.S., Meyer, R.F., Bennett, R.W., Miller, L. and Macmillan, J.D., 1985. In Monoclonal Antibodies Against Bacteria, Vol. II. (Marcario, A.J. and Macario, E.C., eds.) Academic Press pp. 23–59.
Tolbert, W.R., Lewis, Jr., C., White, P.J., and Feder, J., 1985. In “Large Scale Mammalian Cell Culture” (J. Feder and W.R. Tolbert eds.) pp. 97–119, Academic Press.
Van Wezel, A.L., C.A.M. Van der Velden-de Groot, H.H. De Haan, N. Van der Heuvel and R. Schasfoort, 1985, Dev. Biol. Stand. 60, 229.
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Brennan, A.J., Shevitz, J. & Macmillan, J.D. A perfusion system for antibody production by shear-sensitive hybridoma cells in a stirred reactor. Biotechnol Tech 1, 169–174 (1987). https://doi.org/10.1007/BF00227555
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DOI: https://doi.org/10.1007/BF00227555