Abstract
Single-use bioreactors have increasingly been used in recent years, for both research and development as well as industrial production, especially in mammalian cell-based processes. Among the numerous single-use bioreactors available today, wave-mixed bags and stirred systems dominate. Wave-mixed single-use bioreactors are the system of choice for inoculum production, while stirred single-use bioreactors are most often preferred for antibody expression. For this reason, the present chapter describes protocols instructing the reader to use the wave-mixed BIOSTAT® RM 50 for cell expansion and to produce a monoclonal antibody (mAb) in Pall’s Allegro™ STR 200 at pilot scale for the first time. All methods described are based on a Chinese hamster ovary (CHO) suspension cell line expressing a recombinant immunoglobulin G (IgG).
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Acknowledgments
We would like to thank Annelies Onraedt, Michael Schöb, Valentin Rüttimann, Klaus Kienle, Odette Becheau, Joe Capone, Emmanuelle Cameau, Jones Kyle, and John Welsh (all from Pall) for their technical assistance, many helpful discussions, and support. A further thank you goes to Lidija Lisica who strongly supported the ZHAW team in executing the cell expansions and IgG production studies.
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Schirmer, C., Müller, J., Steffen, N., Werner, S., Eibl, R., Eibl, D. (2020). How to Produce mAbs in a Cube-Shaped Stirred Single-Use Bioreactor at 200 L Scale. In: Pörtner, R. (eds) Animal Cell Biotechnology. Methods in Molecular Biology, vol 2095. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0191-4_10
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DOI: https://doi.org/10.1007/978-1-0716-0191-4_10
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