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A genetic map of tetraploid Paspalum notatum Flügge (bahiagrass) based on single-dose molecular markers

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Abstract

Paspalum notatum Flügge is a warm-season forage grass with mainly diploid (2n = 20) and autotetraploid (2n = 40) representatives. Diploid races reproduce sexually and require crosspollination due to a self-incompatible mating system, while autotetraploids reproduce by aposporous apomixis. The objectives of this work were to develop a genetic linkage map of Paspalum notatum Flügge at the tetraploid level, identify the linkage/s group/s associated with apomixis and carry out a general characterization of its mode of inheritance. A pseudo test-cross F1 family of 113 individuals segregating for the mode of reproduction was obtained by crossing a synthetic completely sexual tetraploid plant (Q4188) as female parent with a natural aposporous individual (Q4117) as pollen donor. Map construction was based on single-dose markers (SDAFs) segregating from both parents. Two linkage maps (female and male) were constructed. Within each map, homologous groups were assembled by detecting repulsion-phase linked SDAFs. Putative Q4188 and Q4117 homolog groups were identified by mapping shared single dose markers (BSDF). The Q4188 map consisted of 263 markers distributed on 26 co-segregation groups over a total genetic distance of 1.590.6 cM, while the Q4117 map contained 216 loci dispersed on 39 co-segregation groups along 2.265.7 cM, giving an estimated genome coverage of 88% and 83%, respectively. Seven and 12 putative homologous chromosomes were detected within Q4188 and Q4117 maps, respectively. Afterward, ten female and male homologous chromosomes were identified by mapping BSDFs. In the Q4117 map, a single linkage group was associated with apospory. It was characterized by restriction in recombination and preferential chromosome pairing. A BPSD marker mapping within this group allowed the detection of the female homolog and the putative four male groups of the set carrying apospory.

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References

  • Akiyama Y, Hanna WW, Ozias-Akins P (2005) High resolution physical mapping reveals that the apospory specific genomic region (ASGR) in Cenchrus ciliaris is located on a heterochromatic and hemizygous region of a single chromosome. Theor Appl Genet 111:1042–1051

    Article  PubMed  CAS  Google Scholar 

  • Al-Janabi SM, Honeycutt RJ, McClelland M, Sobral BW (1993) A genetic linkage map of Saccharum spontaneum L. SES 208. Genetics 134:1249–1260

    PubMed  CAS  Google Scholar 

  • Bethel CM, Sciara EB, Estill JC, Bowers JE, Hanna W, Paterson AH (2006) A framework map of bermudagrass (Cynodon dactylon x transvaalensis) based on single-dose restriction fragments. Theor Appl Genet 112:727–737

    Article  PubMed  CAS  Google Scholar 

  • Burton GW (1948) The method of reproduction of common bahiagrass, Paspalum notatum. J Am Soc Agron 40:443–452

    Google Scholar 

  • Burton GW (1967) A search for the origin of Pensacola bahiagrass. Econ Bot 21:379–382

    Google Scholar 

  • Chakravarti A, Lasher LK, Reefer JE (1991) A maximum likelihood method for estimating genome length using genetic linkage data. Genetics 128:175–182

    PubMed  CAS  Google Scholar 

  • Chase A (1929) North American species of Paspalum. Cont US Natl Herb (28): Part 1

  • Cheng R, Kleinhofs A, Ukai Y (1998) Method for mapping a partial lethal-factor locus on a molecular-marker linkage map of a backcross and doubled-haploid population. Theor Appl Genet 97:293–298

    Article  CAS  Google Scholar 

  • Da Silva JAG, Sorrells ME, Burnquist WL, Tanskley SD (1993) RFLP linkage map and genome analysis of Saccharum spontaneum. Genome 36:782–791

    CAS  PubMed  Google Scholar 

  • Daurelio DL, Espinoza F, Quarin CL, Pessino SC (2004) Genetic diversity in sexual diploid and apomictic tetraploid populations of Paspalum notatum situated in sympatry or allopatry. Plant Syst Evol 244:189–199

    Article  CAS  Google Scholar 

  • Ebina M, Nakagawa H, Yamamoto T, Araya H, Tsuruta S, Takahra M, Nakayima K (2005) Co-segregation of AFLP and RAPD markers to apospory in Guineagrass (Panicum maximum Jacq.). Grassland Sci 51:71–78

    Article  CAS  Google Scholar 

  • Espinoza F, Daurelio DL, Pessino SC, Valle EM, Quarin CL (2006) Genetic characterization of Paspalum notatum accessions by AFLP markers. Plant Syst Evol 258:147–159

    Article  CAS  Google Scholar 

  • Forbes I, Burton GW (1961) Cytology of diploids, natural and induced tetraploids, and intraspecific hybrids of bahiagrass, Paspalum notatum. Flugge. Crop Sci 1:402–406

    Article  Google Scholar 

  • Gates RN, Quarin CL, Pedreira CGS (2004) Bahiagrass. In: Moser LE, Burson BL, Sollenberger LE (eds) Warm-season (C4) grasses. ASA, CSSA, and SSSA, Madison, WI pp 651–680

    Google Scholar 

  • Grattapaglia D, Sederoff R (1994) Genetic linkage maps of Eucalyptus grandis and Eucalyptus urophylla using a pseudo test cross: mapping strategy and RAPD markers. Genetics 137:1121–1137

    PubMed  CAS  Google Scholar 

  • Jarret RL, Ozias-Akins P, Phatak S, Nadimpalli R, Duncan R, Hiliard S (1995) DNA contents in Paspalum ssp. determined by flow cytometry. Genet Resour Crop Evol 42:273–242

    Article  Google Scholar 

  • Jessup RW, Burson BL, Burrow G, Wang YW, Chang C, Li Z, Paterson AH, Hussey MA (2003) Segmental allotetraploidy and allelic interactions in buffelgrass (Pennisetum ciliare (L.) Link syn. Cenchrus ciliaris L) as revealed by genome mapping. Genome 46:304–313

    Article  PubMed  CAS  Google Scholar 

  • Kosambi DD (1944) The estimation of map distance from recombination values. Ann Eugen 12:172–175

    Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    Article  PubMed  CAS  Google Scholar 

  • Lerceteau-Kölher E, Guerin G, Laigret F, Denoyes-Rothan B (2003) Characterization of mixed disomic and polysomic inheritance in octoploid strawberry (Fragaria x ananassa) using AFLP mapping. Theor Appl Genet 107(4):619–628

    Article  CAS  Google Scholar 

  • Maliepaard C, Alston FH, van Arkel G, Brown LM, Chevreau E, Dunemann F, Evans KM, Gardiner S, Guilford P, van Heusden AW, Janse J Laurens F, Lynn JR, Manganaris AG, den Nijs APM, Periam N, Rikkerink E, Roche P, Ryder C, Sansavini S, Schimidt H, Tartarini S, Verhaegh JJ, Vrielink-van GM, King GJ (1998) Aligning male and female linkage maps of apple (Malus pumila Mill.) using multi-allelic markers. Theor Appl Genet 97:60–73

    Article  CAS  Google Scholar 

  • Martínez EJ, Urbani MH, Quarin CL, Ortiz JPA (2001) Inheritance of apospory in bahiagrass, Paspalum notatum. Hereditas 135:19–25

    Article  PubMed  Google Scholar 

  • Martínez EJ, Hopp E, Stein J, Ortiz JPA, Quarin CL (2003) Genetic characterization of apospory in tetraploid Paspalum notatum based on the identification of linked molecular markers. Mol Breed 12(4):319–327

    Article  Google Scholar 

  • Missaoui AM, Paterson AH, Bouton JH (2005) Investigation of genomic organization in switchgrass (Panicum virgatum L.) using DNA markers. Theor Appl Genet 110:1372–1383

    Article  PubMed  CAS  Google Scholar 

  • Nogler GA (1984) Gametophytic apomixis. In: Johri BM (ed) Embryology of Angiosperms. Springer-Verlag, Berlin

    Google Scholar 

  • Ortiz JPA, Pessino SC, Bhat V, Hayward MD, Quarin CL (2001) A genetic linkage map of diploid Paspalum notatum. Crop Sci 41:823–830

    Article  CAS  Google Scholar 

  • Pessino SC, Evans C, Ortiz JPA, Armstead I, do Valle CB, Hayward MD (1998) A genetic map of the apospory-region in Brachiaria hybrids: identification of two markers closely associated with the trait. Herditas 128:153–158

    Article  Google Scholar 

  • Pessino SC, Espinoza F, Martínez EJ, Ortiz JPA, Valle E, Quarin CL (2001) Isolation of cDNA clones differentially expressed in flowers of apomictic and sexual Paspalum notatum. Hereditas 134:35–42

    Article  PubMed  CAS  Google Scholar 

  • Porceddu A, Albertini E, Barcaccia E, Falistocco E, Falcinelli M (2002) Linkage mapping in apomictic and sexual Kentucky bluegrass (Poa pratensis L.) genotypes using a two way pseudo-testcross strategy based on AFLP and SAMPL markers. Theor Appl Genet 104:273–280

    Article  PubMed  CAS  Google Scholar 

  • Pupilli F, Martinez EJ, Busti A, Calderini O, Quarin CL, Arcioni S (2004) Comparative mapping reveals partial conservation of synteny at the apomixis locus in Paspalum spp. Mol Gen Genomics 270:539–548

    Article  CAS  Google Scholar 

  • Qu L, Hancock JF (2001) Detecting and mapping repulsion-phase linkage in polyploids with polysomic inheritance. Theor Appl Genet 103:136–143

    Article  CAS  Google Scholar 

  • Quarin CL, Burson BL, Burton GW (1984) Cytology of intra and interspecific hybrids between two cytotypes of Paspalum notatum and P. cromyorrhyzon. Bot Gaz 145:420–426

    Article  Google Scholar 

  • Quarin CL, Urbani MH, Blount AR, Martínez EJ, Hack CM, Burton GW, Quesenberry KH (2003) Registration of Q4188 and Q4205, sexual tetraploid germplasm lines of Bahiagrass. Crop Sci 43:745–746

    Article  Google Scholar 

  • Ripol MI, Churchill GA, Da Silva JAG, Sorrells M (1999) Statistical aspects of genetic mapping in autopolyploids. Gene 235:31–41

    Article  PubMed  CAS  Google Scholar 

  • Ritter E, Gebhardt C, Salamini F (1990) Estimation of recombination frequencies and construction of RFLP linkage maps in plants from crosses between heterozygous parents. Genetics 125:645–654

    PubMed  CAS  Google Scholar 

  • Rodrigues JCM, Cabral GB, Dusi DMA, de Mello LV, Ridgen DJ, Carneiro VTC (2003) Identification of differentially expressed cDNA sequences in ovaries of sexual and apomictic plants of Brachiaria brizantha. Plant Mol Biol 53:745–757

    Article  PubMed  CAS  Google Scholar 

  • Saha MC, Mian R, Zwonitzer JC, Chekhovskiy K, Hopkins AA (2005) An SSR and AFLP based genetic linkage map of tall fescue (Festuca arundinacea Schreb.). Theor Appl Genet 110(2):323–336

    Article  PubMed  CAS  Google Scholar 

  • Stam P (1993) Construction of integrated genetic linkage maps by means of a new computer package: JoinMap. Plant J 3:739–744

    Article  CAS  Google Scholar 

  • Stein J, Quarin CL, Martínez EJ, Pessino SC, Ortiz JPA (2004) Tetraploid races of Paspalum notatum show polysomic inheritance and preferential chromosome pairing around the apospory-controlling locus. Theor Appl Genet 109:186–191

    Article  PubMed  CAS  Google Scholar 

  • Warnke SE, Barker RE, Jung G, Sim S-C, Mian MAR, Saha MC, Brilman LA, Dupal MP, Foster JW (2004) Genetic linkage mapping of an annual x perennial ryegrass population. Theor Appl Genet 109:294–304

    Article  PubMed  CAS  Google Scholar 

  • Wu KK, Burnquist W, Sorrells ME, Tew TL, Moore PH, Tanksley SD (1992) The detection and estimation of linkage in polyploids using single-dose restriction fragments. Theor Appl Genet 83:294–300

    Article  Google Scholar 

Download references

Acknowledgements

Authors wish to thank to Glaucia Cabral and Dr. Vera Tavares de Campos Carneiro, EMBRAPA Cenargen-Brazil for providing the cDNA probes from Brachiaria sp. We thank Ing. Florencia Galdeano for her technical assistance. This study was financed by the Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT), Argentina, PICT N° 13578 and PAV 137/3. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina, PIP No. 6805. Centro Argentino Brasilero de Biotecnología (CABBIO 2003 No. 012). J. Stein and MP Rodriguez received fellowships from CONICET and ANPCyT, respectively. EJ Martínez, SC Pessino, CL Quarin and JPA Ortiz are career members of CONICET.

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Authors and Affiliations

  1. Laboratorio de Biología Molecular, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario (UNR), CC 14, Zavalla, Pcia. de Santa Fe, S2125ZAA, Argentina

    Juliana Stein, Silvina C. Pessino, María Pía Rodriguez, Lorena A. Siena & Juan Pablo Amelio Ortiz

  2. Instituto de Botánica del Nordeste (IBONE)-CONICET, Facultad de Ciencias Agrarias, Universidad Nacional del Nordeste (UNNE), CC 209, Corrientes, 3400, Argentina

    Eric J. Martínez, Camilo L. Quarin & Juan Pablo Amelio Ortiz

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  1. Juliana Stein
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  2. Silvina C. Pessino
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  3. Eric J. Martínez
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  4. María Pía Rodriguez
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  5. Lorena A. Siena
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  6. Camilo L. Quarin
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  7. Juan Pablo Amelio Ortiz
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Correspondence to Juan Pablo Amelio Ortiz.

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Stein, J., Pessino, S.C., Martínez, E.J. et al. A genetic map of tetraploid Paspalum notatum Flügge (bahiagrass) based on single-dose molecular markers. Mol Breeding 20, 153–166 (2007). https://doi.org/10.1007/s11032-007-9083-0

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  • DOI: https://doi.org/10.1007/s11032-007-9083-0

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