Microalgae mixotrophic cultivation for β-galactosidase production

  • Cristina Maria Zanette
  • André Bellin Mariano
  • Yuri Sussumu Yukawa
  • Israel Mendes
  • Michele Rigon SpierEmail author


Microalgae present unexplored biotechnological potential and the ability to use different carbon sources in mixotrophic cultivation. Considering the need for efficient and low-cost industrial processes, the aim of this work was to evaluate the ability of microalgae and cyanobacteria to produce intra- and extracellular β-galactosidase. Eight species of Chlorophyta and Cyanophyta were cultivated in mixotrophic conditions with lactose as a carbon source. Dunaliella tertiolecta, Chlorella minutissima, and Nannochloropsis oculata were able to grow under mixotrophic conditions showing biomass production and growth rates higher than those of photoautotrophic cultures. β-Galactosidase extracellular production was 33.5 U L−1 on the 11th cultivation day for D. tertiolecta. For N. oculata and C. minutissima, the values were 29.6 and 11.02 U L−1 on the 14th and the 7th cultivation days, respectively. This study demonstrates the ability of microalgae to hydrolyze lactose under a mixotrophic regime and to outstanding great potential in the production of biomass and β-galactosidase.


Photosynthetic microorganisms Enzyme Lactose β-Galactosidase 



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© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Technology Sector, Department of Chemical Engineering, Post-Graduate Program in Food EngineeringFederal University of Paraná (UFPR)CuritibaBrazil
  2. 2.Department of Food EngineeringMidwestern State University (UNICENTRO)GuarapuavaBrazil
  3. 3.Technology Sector, Department of Electrical Engineering, Sustainable Energy Research & Development Center (NPDEAS)Federal University of Paraná (UFPR)CuritibaBrazil
  4. 4.Technology Sector, Department of Chemical EngineeringFederal University of ParanáCuritibaBrazil
  5. 5.Technology Institute of Paraná (TECPAR), Environmental and Health Technology CenterCuritibaBrazil

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