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Microalgae mixotrophic cultivation for β-galactosidase production

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Abstract

Microalgae present unexplored biotechnological potential and the ability to use different carbon sources in mixotrophic cultivation. Considering the need for efficient and low-cost industrial processes, the aim of this work was to evaluate the ability of microalgae and cyanobacteria to produce intra- and extracellular β-galactosidase. Eight species of Chlorophyta and Cyanophyta were cultivated in mixotrophic conditions with lactose as a carbon source. Dunaliella tertiolecta, Chlorella minutissima, and Nannochloropsis oculata were able to grow under mixotrophic conditions showing biomass production and growth rates higher than those of photoautotrophic cultures. β-Galactosidase extracellular production was 33.5 U L−1 on the 11th cultivation day for D. tertiolecta. For N. oculata and C. minutissima, the values were 29.6 and 11.02 U L−1 on the 14th and the 7th cultivation days, respectively. This study demonstrates the ability of microalgae to hydrolyze lactose under a mixotrophic regime and to outstanding great potential in the production of biomass and β-galactosidase.

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Authors and Affiliations

  1. Technology Sector, Department of Chemical Engineering, Post-Graduate Program in Food Engineering, Federal University of Paraná (UFPR), Curitiba, Parana, Brazil

    Cristina Maria Zanette & Michele Rigon Spier

  2. Department of Food Engineering, Midwestern State University (UNICENTRO), Guarapuava, Parana, Brazil

    Cristina Maria Zanette

  3. Technology Sector, Department of Electrical Engineering, Sustainable Energy Research & Development Center (NPDEAS), Federal University of Paraná (UFPR), Curitiba, Brazil

    André Bellin Mariano

  4. Technology Sector, Department of Chemical Engineering, Federal University of Paraná, Curitiba, Brazil

    Yuri Sussumu Yukawa

  5. Technology Institute of Paraná (TECPAR), Environmental and Health Technology Center, Curitiba, Parana, Brazil

    Israel Mendes

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  1. Cristina Maria Zanette
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  5. Michele Rigon Spier
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Correspondence to Michele Rigon Spier.

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Zanette, C.M., Mariano, A.B., Yukawa, Y.S. et al. Microalgae mixotrophic cultivation for β-galactosidase production. J Appl Phycol 31, 1597–1606 (2019). https://doi.org/10.1007/s10811-018-1720-y

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