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Growing Phototrophic Cells without Light

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Abstract

Many phototrophic microorganisms contain large quantities of high-value products such as n-3 polyunsaturated fatty acids and carotenoids but phototrophic growth is often slow due to light limitation. Some phototrophic microorganisms can also grow on cheap organic substrate heterotrophically. Heterotrophic cultivation can be well controlled and provides the possibility to achieve fast growth and high yield of valuable products on a large scale. Several strategies have been investigated for cultivation of phototrophic microorganisms without light. These include trophic conversion of obligate photoautotrophic microorganisms by genetic engineering, development of efficient cultivation systems and optimization of culture conditions. This paper reviews recent advances in heterotrophic cultivation of phototrophic cells with an emphasis on microalgae.

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References

  • KE Apt PW Behrens (1999) ArticleTitleCommercial developments in microalgal biotechnology J. Phycol. 35 215–226 Occurrence Handle10.1046/j.1529-8817.1999.3520215.x

    Article  Google Scholar 

  • WR Barclay KM Meager JR Abril (1994) ArticleTitleHeterotrophic production of long-chain omega-3 fatty acids utilizing algae and algae-like microorganisms J. Appl. Phycol. 6 123–129 Occurrence Handle10.1007/BF02186066 Occurrence Handle1:CAS:528:DyaK2cXlslOks7o%3D

    Article  CAS  Google Scholar 

  • AAD Beauclerk AJ Smith (1978) ArticleTitleTransport of d-glucose and 3-O-methyl-d-glucose in cyanobacteria Aphanocapsa 6714 and Nostoc strain MAC Eur. J. Biochem. 82 187–197 Occurrence Handle10.1111/j.1432-1033.1978.tb12011.x Occurrence Handle413715 Occurrence Handle1:CAS:528:DyaE1cXosFOhsQ%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • CR Benedict (1978) ArticleTitleNature of obligate photoautotrophy Annu. Rev. Plant Physiol. Plant Molec. Biol. 29 67–93 Occurrence Handle10.1146/annurev.pp.29.060178.000435 Occurrence Handle1:CAS:528:DyaE1cXktl2gt78%3D

    Article  CAS  Google Scholar 

  • MA Borowitzka (1997) ArticleTitleMicroalgae for aquaculture: opportunities and constraints J. Appl. Phycol. 9 393–401 Occurrence Handle10.1023/A:1007921728300

    Article  Google Scholar 

  • MA Borowitzka (1999) ArticleTitleCommercial production of microalgae: ponds, tanks, tubes and fermenters J. Biotechnol. 70 313–321 Occurrence Handle10.1016/S0168-1656(99)00083-8 Occurrence Handle1:CAS:528:DyaK1MXjs1Shsb0%3D

    Article  CAS  Google Scholar 

  • BR Brinda R Sarada BS Kamath GA Ravishankar (2004) ArticleTitleAccumulation of astaxanthin in flagellated cells of Haematococcus pluvialis – cultural and regulatory aspects Curr. Sci. 87 1290–1295 Occurrence Handle1:CAS:528:DC%2BD2cXhtFChur%2FO

    CAS  Google Scholar 

  • G Britton (1995) ArticleTitleStructure and properties of carotenoids in relation to function FASEB J. 9 1551–1558 Occurrence Handle8529834 Occurrence Handle1:CAS:528:DyaK28XhsVCksw%3D%3D

    PubMed  CAS  Google Scholar 

  • F Chen (1996) ArticleTitleHigh cell density culture of microalgae in heterotrophic growth Trends Biotechnol. 14 421–426 Occurrence Handle10.1016/0167-7799(96)10060-3 Occurrence Handle1:CAS:528:DyaK28XntVyltb4%3D

    Article  CAS  Google Scholar 

  • F Chen MR Johns (1991) ArticleTitleEffect of C/N ratio and aeration on the fatty acid composition of heterotrophic Chlorella sorokiniana J. Appl. Phycol. 3 203–209 Occurrence Handle1:CAS:528:DyaK38XhtVansrY%3D

    CAS  Google Scholar 

  • F Chen MR Johns (1996) ArticleTitleHeterotrophic growth of Chlamydomonas reinhardtii on acetate in chemostat culture Proc. Biochem. 31 601–604 Occurrence Handle10.1016/S0032-9592(96)00006-4 Occurrence Handle1:CAS:528:DyaK28Xjs1ehs7g%3D

    Article  CAS  Google Scholar 

  • YE Choi YS Yun JM Park (2002) ArticleTitleEvaluation of factors promoting astaxanthin production by a unicellular green alga, Haematococcus pluvialis, with fractional factorial design Biotechnol. Prog. 18 1170–1175 Occurrence Handle12467447 Occurrence Handle10.1021/bp025549b Occurrence Handle1:CAS:528:DC%2BD38XmsFGms7k%3D

    Article  PubMed  CAS  Google Scholar 

  • Cohen Z, Ratledge C (2005) (eds): Single Cell Oils, Champaign, IL, AOCS Press

  • ME Swaaf Particlede TC Rijk Particlede G Eggink L Sijtsma (1999) ArticleTitleOptimisation of docosahexaenoic acid production in batch cultivations by Crypthecodinium cohnii J. Biotechnol. 70 185–192 Occurrence Handle10.1016/S0168-1656(99)00071-1

    Article  Google Scholar 

  • ME Swaaf Particlede JT Pronk L Sijtsma (2003a) ArticleTitleFed-batch cultivation of the docosahexaenoic-acid-producing marine alga Crypthecodinium cohnii on ethanol Appl. Microbiol. Biotechnol. 61 40–43

    Google Scholar 

  • ME Swaaf Particlede L Sijtsma JT Pronk (2003b) ArticleTitleHigh-cell-density fed-batch cultivation of the docosahexaenoic acid producing marine alga Crypthecodinium cohnii Biotechnol. Bioeng. 81 666–672 Occurrence Handle10.1002/bit.10513 Occurrence Handle1:CAS:528:DC%2BD3sXhtlOnu70%3D

    Article  CAS  Google Scholar 

  • KW Fan F Chen EBG Jones LLP Vrijmoed (2001) ArticleTitleEicosapentaenoic and docosahexaenoic acids production by and okara-utilizing potential of thraustochytrids J. Ind. Microbiol. Biotechnol. 27 199–202 Occurrence Handle11687930 Occurrence Handle10.1038/sj.jim.7000169 Occurrence Handle1:CAS:528:DC%2BD3MXotFWns7s%3D

    Article  PubMed  CAS  Google Scholar 

  • H Fischer I Robl M Sumper N Kroger (1999) ArticleTitleTargeting and covalent modification of cell wall and membrane proteins heterologously expressed in the diatom Cylindrotheca fusiformis (Bacillariophyceae) J. Phycol. 35 113–120 Occurrence Handle10.1046/j.1529-8817.1999.3510113.x Occurrence Handle1:CAS:528:DyaK1MXhvVKrsL0%3D

    Article  CAS  Google Scholar 

  • M Guerin ME Huntley M Olaizola (2003) ArticleTitleHaematococcus astaxanthin: applications for human health and nutrition Trends Biotechnol. 21 210–216 Occurrence Handle10.1016/S0167-7799(03)00078-7 Occurrence Handle12727382 Occurrence Handle1:CAS:528:DC%2BD3sXjtlWltLw%3D

    Article  PubMed  CAS  Google Scholar 

  • A Hallmann M Sumper (1996) ArticleTitleThe Chlorella hexose/H+ symporter is a useful selectable marker and biochemical reagent when expressed in Volvox Proc. Natl. Acad. Sci. USA 93 669–673 Occurrence Handle10.1073/pnas.93.2.669 Occurrence Handle8570613 Occurrence Handle1:CAS:528:DyaK28XnslOmtA%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • M Harel W Koven I Lein Y Bar P Behrens J Stubblefield Y Zohar AR Place (2002) ArticleTitleAdvanced DHA, EPA and ArA enrichment materials for marine aquaculture using single cell heterotrophs Aquaculture 213 347–362 Occurrence Handle10.1016/S0044-8486(02)00047-9 Occurrence Handle1:CAS:528:DC%2BD38Xnt1GjsLw%3D

    Article  CAS  Google Scholar 

  • PF Ip F Chen (2005a) ArticleTitleProduction of astaxanthin by the green microalga Chlorella zofingiensis in the dark Proc. Biochem. 40 733–738 Occurrence Handle10.1016/j.procbio.2004.01.039 Occurrence Handle1:CAS:528:DC%2BD2cXpt1Wgsbg%3D

    Article  CAS  Google Scholar 

  • PF Ip F Chen (2005b) ArticleTitleEmployment of reactive oxygen species to enhance astaxanthin formation in Chlorella zofingiensis in heterotrophic culture Proc. Biochem. 40 3491–3496 Occurrence Handle10.1016/j.procbio.2005.02.014 Occurrence Handle1:CAS:528:DC%2BD2MXhtVeiurbI

    Article  CAS  Google Scholar 

  • PF Ip F Chen (2005c) ArticleTitlePeroxynitrite and nitryl chloride enhance astaxanthin production by the green microalga Chlorella zofingiensis in heterotrophic culture Proc. Biochem. 40 3595–3599 Occurrence Handle10.1016/j.procbio.2005.03.055 Occurrence Handle1:CAS:528:DC%2BD2MXhtVeiurfE

    Article  CAS  Google Scholar 

  • Y Jiang F Chen (1999) ArticleTitleEffects of salinity on cell growth and docosahexaenoic acid content of the heterotrophic marine microalga Crypthecodinium cohnii J. Ind. Microbiol. Biotechnol. 23 508–513 Occurrence Handle10.1038/sj.jim.2900759 Occurrence Handle1:CAS:528:DC%2BD3cXlsFOhtA%3D%3D

    Article  CAS  Google Scholar 

  • Y Jiang F Chen (2000a) ArticleTitleEffects of temperature and temperature shift on docosahexaenoic acid production by the marine microalga Crypthecodinium cohnii J. Am. Oil Chem. Soc. 77 613–617 Occurrence Handle1:CAS:528:DC%2BD3cXksVOgu7w%3D

    CAS  Google Scholar 

  • Y Jiang F Chen (2000b) ArticleTitleEffects of medium glucose concentration and pH on docosahexaenoic acid content of heterotrophic Crypthecodinium cohnii Process Biochem. 35 1205–1209 Occurrence Handle10.1016/S0032-9592(00)00163-1 Occurrence Handle1:CAS:528:DC%2BD3cXlt1Witro%3D

    Article  CAS  Google Scholar 

  • Y Jiang KW Fan RDY Wong F Chen (2004) ArticleTitleFatty acid composition and squalene content of the marine microalga Schizochytrium mangrovei J. Agric. Food Chem. 52 1196–1200 Occurrence Handle14995120 Occurrence Handle1:CAS:528:DC%2BD2cXnsVKmsg%3D%3D

    PubMed  CAS  Google Scholar 

  • EJ Johnson (2004) ArticleTitleA biological role of lutein Food Rev. Int. 20 1–16 Occurrence Handle10.1081/FRI-120028826 Occurrence Handle1:CAS:528:DC%2BD2cXksFGitrs%3D

    Article  CAS  Google Scholar 

  • M Kobayashi Y Kurimura Y Tsuji (1997) ArticleTitleLight-independent, astaxanthin production by the green microalga Haematococcus pluvialis under salt stress Biotechnol. Lett. 19 507–509 Occurrence Handle10.1023/A:1018372900649 Occurrence Handle1:CAS:528:DyaK2sXktF2nu7s%3D

    Article  CAS  Google Scholar 

  • PM Kris-Etherton KD Hecker AE Binkoski (2004) ArticleTitlePolyunsaturated fatty acids and cardiovascular health Nutr. Rev. 62 414–426 Occurrence Handle10.1301/nr.2004.nov.414-426 Occurrence Handle15622714

    Article  PubMed  Google Scholar 

  • DW Lawlor (2001) Photosynthesis EditionNumber3 BIOS Scientific Publishers Ltd Oxford

    Google Scholar 

  • YK Lee (1997) ArticleTitleCommercial production of microalgae in the Asia-Pacific rim J. Appl. Phycol. 9 403–411 Occurrence Handle10.1023/A:1007900423275

    Article  Google Scholar 

  • YK Lee (2001) ArticleTitleMicroalgal mass culture systems and methods: their limitation and potential J. Appl. Phycol. 13 307–315 Occurrence Handle10.1023/A:1017560006941

    Article  Google Scholar 

  • RA Lewin (1962) Physiology and Biochemistry of Algae EditionNumber1 Academic Press New York

    Google Scholar 

  • BH Liu YK Lee (2000) ArticleTitleSecondary carotenoids formation by the green alga Chlorococcum sp J. Appl. Phycol. 12 301–307 Occurrence Handle10.1023/A:1008185212724 Occurrence Handle1:CAS:528:DC%2BD3cXoslejtLc%3D

    Article  CAS  Google Scholar 

  • RYM Ma F Chen (2001) ArticleTitleInduction of astaxanthin formation by reactive oxygen species in mixotrophic culture of Chlorococcum sp Biotechnol. Lett. 23 519–523 Occurrence Handle10.1023/A:1010370401235 Occurrence Handle1:CAS:528:DC%2BD3MXjtVejtr4%3D

    Article  CAS  Google Scholar 

  • H Nishino M Murakoshi T Ii M Takemura M Kuchide M Kanazawa XY Mou S Wada M Masuda Y Ohsaka S Yogosawa Y Satomi K Jinno (2002) ArticleTitleCarotenoids in cancer chemoprevention Cancer Metast. Rev. 21 257–264 Occurrence Handle10.1023/A:1021206826750 Occurrence Handle1:CAS:528:DC%2BD38XptFejs74%3D

    Article  CAS  Google Scholar 

  • M Olaizola (2003) ArticleTitleCommercial development of microalgal biotechnology: from the test tube to the marketplace Biomol. Eng. 20 459–466 Occurrence Handle12919832 Occurrence Handle10.1016/S1389-0344(03)00076-5 Occurrence Handle1:CAS:528:DC%2BD3sXmt1Khsbs%3D

    Article  PubMed  CAS  Google Scholar 

  • B Raboy E Padan (1978) ArticleTitleActive transport of glucose and alpha-methylglucoside in cyanobacterium Plectonema boryanum J. Biol. Chem. 253 3287–3291 Occurrence Handle417083 Occurrence Handle1:CAS:528:DyaE1cXktVeju7w%3D

    PubMed  CAS  Google Scholar 

  • C Ratledge (2004) ArticleTitleFatty acid biosynthesis in microorganisms being used for single cell oil production Biochimie 86 807–815 Occurrence Handle10.1016/j.biochi.2004.09.017 Occurrence Handle15589690 Occurrence Handle1:CAS:528:DC%2BD2cXhtVKksLbK

    Article  PubMed  CAS  Google Scholar 

  • C Ratledge K Kanagachandran AJ Anderson DJ Grantham JC Stephenson (2001) ArticleTitleProduction of docosahexaenoic acid by Crypthecodinium cohnii grown in a pH-auxostat culture with acetic acid as principal carbon source Lipids 36 1241–1246 Occurrence Handle11795857 Occurrence Handle1:CAS:528:DC%2BD38Xht1Oruw%3D%3D

    PubMed  CAS  Google Scholar 

  • CHS Ruxton SC Reed MJA Simpson KJ Millington (2004) ArticleTitleThe health benefits of omega-3 polyunsaturated fatty acids: a review of the evidence J. Hum. Nutr. Diet. 17 449–459 Occurrence Handle10.1111/j.1365-277X.2004.00552.x Occurrence Handle15357699 Occurrence Handle1:STN:280:DC%2BD2cvltVGhtw%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • CHS Ruxton PC Calder SC Reed MJA Simpson (2005) ArticleTitleThe impact of long-chain n-3 polyunsaturated fatty acids on human health Nutr. Res. Rev. 18 113–129 Occurrence Handle10.1079/NRR200497 Occurrence Handle1:CAS:528:DC%2BD2MXntFSktb4%3D

    Article  CAS  Google Scholar 

  • RA Schmidt MG Wiebe NT Eriksen (2005) ArticleTitleHeterotrophic high cell-density fed-batch cultures of the phycocyanin-producing red alga Galdieria sulphuraria Biotechnol. Bioeng. 90 77–84 Occurrence Handle10.1002/bit.20417 Occurrence Handle15723314 Occurrence Handle1:CAS:528:DC%2BD2MXivVamtrc%3D

    Article  PubMed  CAS  Google Scholar 

  • XM Shi HJ Liu XW Zhang F Chen (1999) ArticleTitleProduction of biomass and lutein by Chlorella protothecoides at various glucose concentrations in heterotrophic cultures Proc. Biochem. 34 341–347 Occurrence Handle10.1016/S0032-9592(98)00101-0 Occurrence Handle1:CAS:528:DyaK1MXksVSrtbg%3D

    Article  CAS  Google Scholar 

  • XM Shi XW Zhang F Chen (2000) ArticleTitleHeterotrophic production of biomass and lutein by Chlorella protothecoides on various nitrogen sources Enzyme Microb. Technol. 27 312–318 Occurrence Handle10.1016/S0141-0229(00)00208-8 Occurrence Handle10899558 Occurrence Handle1:CAS:528:DC%2BD3cXkslSjt7k%3D

    Article  PubMed  CAS  Google Scholar 

  • XM Shi Y Jiang F Chen (2002) ArticleTitleHigh-yield production of lutein by the green microalga Chlorella protothecoides in heterotrophic fed-batch culture Biotechnol. Prog. 18 723–727 Occurrence Handle10.1021/bp0101987 Occurrence Handle12153304 Occurrence Handle1:CAS:528:DC%2BD38XltlCnsLY%3D

    Article  PubMed  CAS  Google Scholar 

  • H Sies W Stahl (2004) ArticleTitleCarotenoids and UV protection Photochem. Photobiol. Sci. 3 749–752 Occurrence Handle10.1039/b316082c Occurrence Handle15295630 Occurrence Handle1:CAS:528:DC%2BD2cXmt1Gjtb4%3D

    Article  PubMed  CAS  Google Scholar 

  • L Sijtsma ME Swaaf Particlede (2004) ArticleTitleBiotechnological production and applications of the omega-3 polyunsaturated fatty acid docosahexaenoic acid Appl. Microbiol. Biotechnol. 64 146–153 Occurrence Handle10.1007/s00253-003-1525-y Occurrence Handle14740186 Occurrence Handle1:CAS:528:DC%2BD2cXis1Gls7c%3D

    Article  PubMed  CAS  Google Scholar 

  • W Stahl H Sies (2005) ArticleTitleBioactivity and protective effects of natural carotenoids Biochim. Biophys. Acta 1740 101–107 Occurrence Handle15949675 Occurrence Handle1:CAS:528:DC%2BD2MXltFCrsb8%3D

    PubMed  CAS  Google Scholar 

  • EE Stinson R Kwoczak MJ Kurantz (1991) ArticleTitleEffect of cultural conditions on production of eicosapentaenoic acid by Pythium irregulare J. Ind. Microbiol. 8 171–178 Occurrence Handle1367900 Occurrence Handle10.1007/BF01575850 Occurrence Handle1:CAS:528:DyaK38Xlt1en

    Article  PubMed  CAS  Google Scholar 

  • U Tripathi R Sarada GA Ravishankar (2002) ArticleTitleEffect of culture conditions on growth of green alga Haematococcus pluvialis and astaxanthin production Acta Physiol. Plant. 24 323–329 Occurrence Handle1:CAS:528:DC%2BD38XpsV2lsrg%3D

    CAS  Google Scholar 

  • HC Vogel CL Todaro (1997) Fermentation and Biochemical Engineering Handbook: Principles, Process Design, and Equipment EditionNumber2 Noyes Publications Westwood, New Jersey

    Google Scholar 

  • Wen ZY (2001) A high yield and productivity strategy for eicosapentaenoic acid production by the diatom Nitzschia laevis in heterotrophic culture. PhD Thesis. Hong Kong: The University of Hong Kong

  • ZY Wen F Chen (2000) ArticleTitleHeterotrophic production of eicosapentaenoid acid by the diatom Nitzschia laevis: effects of silicate and glucose J. Ind. Microbiol. Biotechnol. 25 218–224 Occurrence Handle10.1038/sj.jim.7000056 Occurrence Handle1:CAS:528:DC%2BD3MXlvF2ntg%3D%3D

    Article  CAS  Google Scholar 

  • ZY Wen F Chen (2001a) ArticleTitleOptimization of nitrogen sources for heterotrophic production of eicosapentaenoic acid by the diatom Nitzschia laevis Enzyme Microb. Technol. 29 341–347 Occurrence Handle10.1016/S0141-0229(01)00385-4 Occurrence Handle1:CAS:528:DC%2BD3MXnt1Whu7k%3D

    Article  CAS  Google Scholar 

  • ZY Wen F Chen (2001b) ArticleTitleApplication of statistically-based experimental designs for the optimization of eicosapentaenoic acid production by the diatom Nitzschia laevis Biotechnol. Bioeng. 75 159–169 Occurrence Handle10.1002/bit.1175 Occurrence Handle1:CAS:528:DC%2BD3MXnsVeqsbs%3D

    Article  CAS  Google Scholar 

  • ZY Wen F Chen (2002a) ArticleTitlePerfusion culture of the diatom Nitzschia laevis for ultra-high yield of eicosapentaenoic acid Proc. Biochem. 38 523–529 Occurrence Handle10.1016/S0032-9592(02)00174-7 Occurrence Handle1:CAS:528:DC%2BD3sXhtFaltA%3D%3D

    Article  CAS  Google Scholar 

  • ZY Wen F Chen (2002b) ArticleTitleContinuous cultivation of the diatom Nitzschia laevis for eicosapentaenoic acid production: physiological study and process optimization Biotechnol. Prog. 18 21–28 Occurrence Handle10.1021/bp010125n Occurrence Handle1:CAS:528:DC%2BD3MXptlWnsL0%3D

    Article  CAS  Google Scholar 

  • ZY Wen F Chen (2003) ArticleTitleHeterotrophic production of eicosapentaenoic acid by microalgae Biotechnol. Adv. 21 273–294 Occurrence Handle10.1016/S0734-9750(03)00051-X Occurrence Handle14499126 Occurrence Handle1:CAS:528:DC%2BD3sXkvF2gs7s%3D

    Article  PubMed  CAS  Google Scholar 

  • ZY Wen Y Jiang F Chen (2002) ArticleTitleHigh cell density culture of the diatom Nitzschia laevis for eicosapentaenoic acid production: fed-batch development Proc. Biochem. 37 1447–1453 Occurrence Handle10.1016/S0032-9592(02)00034-1 Occurrence Handle1:CAS:528:DC%2BD38XktVyjtLw%3D

    Article  CAS  Google Scholar 

  • AP Wood JP Aurikko DP Kelly (2004) ArticleTitleA challenge for 21st century molecular biology and biochemistry: what are the causes of obligate autotrophy and methanotrophy? FEMS Microbiol. Rev. 28 335–352 Occurrence Handle10.1016/j.femsre.2003.12.001 Occurrence Handle15449607 Occurrence Handle1:CAS:528:DC%2BD2cXks1equ7w%3D

    Article  PubMed  CAS  Google Scholar 

  • T Yaguchi S Tanaka T Yokochi T Nakahara T Higashihara (1997) ArticleTitleProduction of high yields of docosahexaenoic acid by Schizochytrium sp. strain SR21 J. Am. Oil Chem. Soc. 74 1431–1434 Occurrence Handle1:CAS:528:DyaK2sXnsFGrtr8%3D

    CAS  Google Scholar 

  • T Yokochi D Honda T Higashihara T Nakahara (1998) ArticleTitleOptimization of docosahexaenoic acid production by Schizochytrium limacinum SR21 Appl. Microbiol. Biotechnol. 49 72–76 Occurrence Handle10.1007/s002530051139 Occurrence Handle1:CAS:528:DyaK1cXhtVantbc%3D

    Article  CAS  Google Scholar 

  • W Yongmanitchai OP Ward (1991) ArticleTitleGrowth of and omega-3 fatty acid production by Phaeodactylum tricornutum under different culture conditions Appl. Environ. Microbiol. 57 419–425 Occurrence Handle2014989 Occurrence Handle1:CAS:528:DyaK3MXpsl2rsA%3D%3D

    PubMed  CAS  Google Scholar 

  • LA Zaslavskaia JC Lippmeier C Shih D Ehrhardt AR Grossman KE Apt (2001) ArticleTitleTrophic conversion of an obligate photoautotrophic organism through metabolic engineering Science 292 2073–2075 Occurrence Handle10.1126/science.160015 Occurrence Handle11408656 Occurrence Handle1:STN:280:DC%2BD3MzksFygug%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • CC Zhang R Jeanjean F Joset (1998) ArticleTitleObligate phototrophy in cyanobacteria: more than a lack of sugar transport FEMS Microbiol. Lett. 161 285–292 Occurrence Handle9570120 Occurrence Handle1:CAS:528:DyaK1cXhvFKjs7o%3D

    PubMed  CAS  Google Scholar 

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Chen, GQ., Chen, F. Growing Phototrophic Cells without Light. Biotechnol Lett 28, 607–616 (2006). https://doi.org/10.1007/s10529-006-0025-4

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