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Strain and plastic composite support (PCS) selection for vitamin K (Menaquinone-7) production in biofilm reactors

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Abstract

Menaquinone-7 (MK-7), a subtype of vitamin K, has received a significant attention due to its effect on improving bone and cardiovascular health. Current fermentation strategies, which involve static fermentation without aeration or agitation, are associated with low productivity and scale-up issues and hardly justify the commercial production needs of this vitamin. Previous studies indicate that static fermentation is associated with pellicle and biofilm formations, which are critical for MK-7 secretion while posing significant operational issues. Therefore, the present study is undertaken to evaluate the possibility of using a biofilm reactor as a new strategy for MK-7 fermentation. Bacillus species, namely, Bacillus subtilis natto, Bacillus licheniformis, and Bacillus amyloliquifaciens as well as plastic composite, supports (PCS) were investigated in terms of MK-7 production and biofilm formation. Results show the possibility of using a biofilm reactor for MK-7 biosynthesis. Bacillus subtilis natto and soybean flour yeast extract PCS in glucose medium were found as the most potent combination for production of MK-7 as high as 35.5 mg/L, which includes both intracellular and extracellular MK-7.

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Acknowledgements

This project was partly funded by the Pennsylvania Agricultural Experiment Station. The authors thank the Statistical Consulting Center at Pennsylvania State University for assisting data processing.

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Authors and Affiliations

  1. Department of Agricultural and Biological Engineering, The Pennsylvania State University, University Park, PA, 16802, USA

    Ehsan Mahdinia & Ali Demirci

  2. Faculty of Science and Engineering, The University of Waikato, Hamilton, 3240, New Zealand

    Aydin Berenjian

Authors
  1. Ehsan Mahdinia
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  2. Ali Demirci
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  3. Aydin Berenjian
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Correspondence to Ali Demirci.

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Mahdinia, E., Demirci, A. & Berenjian, A. Strain and plastic composite support (PCS) selection for vitamin K (Menaquinone-7) production in biofilm reactors. Bioprocess Biosyst Eng 40, 1507–1517 (2017). https://doi.org/10.1007/s00449-017-1807-x

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