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Stimulation of bikaverin production by sucrose and by salt starvation in Fusarium fujikuroi

  • Applied Microbial and Cell Physiology
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Abstract

The fungus Fusarium fujikuroi (Gibberella fujikuroi mating group C) exhibits a rich secondary metabolism that includes the synthesis of compounds of biotechnological interest, such as gibberellins, bikaverin, and carotenoids. The effect of the carbon source on their production was checked using a two-phase incubation protocol, in which nine different sugars were added upon transfer of the fungus from repressed to appropriate inducing conditions, i.e., nitrogen starvation for gibberellins and bikaverin and illumination for carotenoids production. Most of the carbon sources allowed the synthesis of these metabolites in significant amounts. However, bikaverin production was strongly increased by the presence of sucrose in comparison to other carbon sources, an effect not exhibited for the production of gibberellins and carotenoids. The bikaverin inducing effect was enhanced in the absence of phosphate and/or sulfate. Similar results were also observed in carotenoid-overproducing strains known to be altered in bikaverin production. The induction by salt starvation, but not by sucrose, correlated with an increase in messenger RNA levels of gene bik1, encoding a polyketide synthase of the bikaverin pathway.

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Acknowledgments

We are grateful to Sonia Distel for technical assistance. This work was supported by a Marie Curie reintegration grant (contract MERG-CT-2004-006378), Spanish Government (Ministerio de Ciencia y Tecnología, projects BIO2003-01548 and BIO2006-01323), and Andalusian Government (Consejería de Innovación, Ciencia y Empresa, project P07-CVI-02813). R. Rodríguez-Ortiz was supported by a CONACYT fellowship from the Government of Mexico.

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  1. Departamento de Genética, Universidad de Sevilla, Apartado 1095, 41080, Sevilla, Spain

    Roberto Rodríguez-Ortiz, Bina J. Mehta, Javier Avalos & M. Carmen Limón

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  1. Roberto Rodríguez-Ortiz
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  2. Bina J. Mehta
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  3. Javier Avalos
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  4. M. Carmen Limón
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Correspondence to M. Carmen Limón.

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Rodríguez-Ortiz, R., Mehta, B.J., Avalos, J. et al. Stimulation of bikaverin production by sucrose and by salt starvation in Fusarium fujikuroi . Appl Microbiol Biotechnol 85, 1991–2000 (2010). https://doi.org/10.1007/s00253-009-2282-3

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