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Improved GA1 production by Fusarium fujikuroi

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Abstract.

Recent studies into gibberellin A1 (GA1) showed it to be physiologically more active than GA3 in plants of great agricultural interest, such as tomatoes, rice, peas, and sweet cherries. We describe here a simple procedure for obtaining large quantities of GA1 (1,500 mg/l) by incubating the FKMC1995 strain of Fusarium fujikuroi in a standard complex medium (SCM). We also compare the GA production of this strain with that of two other wild-type strains of F. fujikuroi (IMI58289, m567) in SCM and low-nitrogen medium and discuss the possible biogenetic mechanisms involved in the over-accumulation of GA1 by FKMC1995.

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Acknowledgements.

This research was financed by the European Community (FEDER programme) project 1FD97-1346-C02-01. We thank Prof. E. Cerdá-Olmedo for his collaboration and suggestions, Dr. J. F. Leslie and Dr. B. Tudzynski for strains FKMC1995 and m567 respectively, Dr. M. Muñoz-Dorado for the facilities provided, J. F. Fernández-Sánchez and A. Salinas-Castillo for the spectrofluorimetric analyses, and our English colleague Dr. J. Trout for revising style and grammar. The procedures described here for producing GA1 are covered under Spanish patent P200203050. The experiments here described comply with the current laws of Spain.

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Authors and Affiliations

  1. Departamento de Química Orgánica, Instituto de Biotecnología, Facultad de Ciencias, Universidad de Granada, 18071, Granada, Spain

    J. L. Oller-López, A. F. Barrero & J. E. Oltra

  2. Departamento de Genética, Facultad de Biología, Universidad de Sevilla, 41080, Sevilla, Spain

    J. Avalos

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  1. J. L. Oller-López
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  2. J. Avalos
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  3. A. F. Barrero
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  4. J. E. Oltra
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Correspondence to J. E. Oltra.

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Oller-López, J.L., Avalos, J., Barrero, A.F. et al. Improved GA1 production by Fusarium fujikuroi . Appl Microbiol Biotechnol 63, 282–285 (2003). https://doi.org/10.1007/s00253-003-1376-6

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  • DOI: https://doi.org/10.1007/s00253-003-1376-6

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