Abstract
Key message
Two QTL with pleiotropic effects on plant height and spike length linked in coupling phase on chromosome 2DS were dissected, and diagnostic marker for each QTL was developed.
Abstract
Plant height (PHT) is a crucial trait related to plant architecture and yield potential, and dissection of its underlying genetic basis would help to improve the efficiency of designed breeding in wheat. Here, two quantitative trait loci (QTL) linked in coupling phase on the short arm of chromosome 2D with pleiotropic effects on PHT and spike length, QPht/Sl.cau-2D.1 and QPht/Sl.cau-2D.2, were separated and characterized. QPht/Sl.cau-2D.1 is a novel QTL located between SNP makers BS00022234_51 and BobWhite_rep_c63957_1472. QPht/Sl.cau-2D.2 is mapped between two SSR markers, SSR-2062 and Xgwm484, which are located on the same genomic interval as Rht8. Moreover, the diagnostic marker tightly linked with each QTL was developed for the haplotype analysis using diverse panels of wheat accessions. The frequency of the height-reduced allele of QPht/Sl.cau-2D.1 is much lower than that of QPht/Sl.cau-2D.2, suggesting that this novel QTL may be an attractive target for genetic improvement. Consistent with a previous study of Rht8, a significant difference in cell length was observed between the NILs of QPht/Sl.cau-2D.2. By contrast, there was no difference in cell length between NILs of QPht/Sl.cau-2D.1, indicating that the underlying molecular mechanism for these two QTL may be different. Collectively, these data provide a new example of QTL dissection, and the developed diagnostic markers will be useful in marker-assisted pyramiding of QPht/Sl.cau-2D.1 and/or QPht/Sl.cau-2D.2 with the other genes in wheat breeding.
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Acknowledgements
This work was financially supported by the National Key Research and Development Program of China (Grant No. 2017YFD0101004) and the National Natural Science Foundation of China (Grant No. 91435204). The authors would like to thank the IWGSC for pre-publication access to the RefSeq v1.0 sequence.
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ZN and AZ conceived the project; LC, ZC and RB carried out experiments; XC and HZ participated in field trials; LC, ZC and XC performed marker development of the QTL region of interest; LC and ZH developed the segregation populations and near isogenic lines; LC, ZC and RB performed phenotyping of the segregation populations and the near isogenic lines; QS, HP, YY, ZH, MX and WG assisted in revising the manuscript; LC analysed experimental results and wrote the manuscript.
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Chai, L., Chen, Z., Bian, R. et al. Dissection of two quantitative trait loci with pleiotropic effects on plant height and spike length linked in coupling phase on the short arm of chromosome 2D of common wheat (Triticum aestivum L.). Theor Appl Genet 131, 2621–2637 (2018). https://doi.org/10.1007/s00122-018-3177-4
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DOI: https://doi.org/10.1007/s00122-018-3177-4