Skip to main content
SpringerLink
Log in

Detection of genetic determinants that define the difference in photoperiod sensitivity of Triticum aestivum L. near-isogenic lines

  • Plant Genetics
  • Published:
Russian Journal of Genetics Aims and scope Submit manuscript

Abstract

Identification of genetic determinants that define different degrees of line sensitivity to the photoperiod was acomplished using near-isogenic lines of the soft hexaploid wheat Triticum aestivum L. using SSR markers and markers specific to the Vrn and Ppd genes. It was established that the Ppd-s line contains a dominant Ppd-D1a allele located on chromosome 2D. This allele is characterized by a large deletion in the gene promoter region. For two other lines (Ppd-m and Ppd-w), introgression of the Ppd-B1 gene on chromosome 2B was detected from the parental Sonora variety, which is insensitive to the day length; however, the previously described Ppd-B1a.1 allele was not found. Another polymorphism that can cause weak photoperiodic sensitivity, an increased copy number of the Ppd-B1 gene, was detected for these lines.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Worland, A.J., The influence of flowering time genes on environmental adaptability in European wheats, Euphytica, 1996, vol. 89, no. 1, pp. 49–57.

    Article  Google Scholar 

  2. Borlaug, N.E., Contributions of conventional plant breeding to food production, Science, 1983, vol. 219, no. 4585, pp. 689–693.

    Article  CAS  PubMed  Google Scholar 

  3. Beales, J., Turner, A., Griffiths, S., et al., A pseudoresponse regulator is misexpressed in the photoperiod insensitive Ppd-D1a mutant of wheat (Triticum aestivum L.), Theor. Appl. Genet., 2007, vol. 115, no. 5, pp. 721–733.

    Article  CAS  PubMed  Google Scholar 

  4. Worland, A., Appendino, M., and Sayers, E., The distribution, in European winter wheats, of genes that influence ecoclimatic adaptability whilst determining photoperiodic insensitivity and plant height, Euphytica, 1994, vol. 80, no. 3, pp. 219–228.

    Article  Google Scholar 

  5. Koshkin, V.A., Matvienko, I.I., Egorova, E.M., et al., The use of allel-specific markers of the Ppd-D1 gene for the analysis of isogenic line of spring bread wheat, Tr. Prikl. Bot., Genet. Sel., 2009, vol. 166, pp. 151–156.

    Google Scholar 

  6. McIntosh, R.A., Yamazaki, Y., Devos, K.M., et al., Catalogue of Gene Symbols in Wheat, 2003. http://wheat.pw.usda.gov/ggpages/wgc/2003/

    Google Scholar 

  7. Merezhko, A.F., Koshkin, V.A., and Matvienko, I.I., Initial material for creation of a series of nearly isogenic lines in common wheat with different photoperiodic response, Russ. J. Genet., 1997, vol. 33, no. 4, pp. 410–418.

    CAS  Google Scholar 

  8. Koshkin, V.A., Merezhko, A.F., and Matvienko, I.I., Influence of the photoperiod and Ppd genes on the morphophysiological features of the homozygous lines of wheat with a different photoperiodic sensitivity, Dokl. Ross. Akad. S-kh. Nauk, 1998, no. 4, pp. 8–10.

    Google Scholar 

  9. Saghai-Maroof, M.A., Soliman, K.M., Jorgensen, R.A., and Allard, R.W., Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics, Proc. Natl. Acad. Sci. USA, 1984, vol. 81, pp. 8014–8018.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  10. Röber, M.S., Korzun, V., Wendehake, K., et al., A microsatellite map of wheat, Genetics, 1998, vol. 149, no. 4, pp. 2007–2023.

    Google Scholar 

  11. Song, Q., Shi, J., Singh, S., et al., Development and mapping of microsatellite (SSR) markers in wheat, Theor. Appl. Genet., 2005, vol. 110, no. 3, pp. 550–560.

    Article  CAS  PubMed  Google Scholar 

  12. Yan, L., Helguera, M., Kato, K., et al., Allelic variation at the VRN-1 promoter region in polyploid wheat, Theor. Appl. Genet., 2004, vol. 109, no. 8, pp. 1677–1686.

    Article  CAS  PubMed  Google Scholar 

  13. Fu, D., Szucs, P., Yan, L., et al., Large deletions within the first intron in VRN-1 are associated with spring growth habit in barley and wheat, Mol. Genet. Genomics, 2005, vol. 273, pp. 54–65.

    Article  CAS  PubMed  Google Scholar 

  14. Zhang, X.K., Xiao, Y.G., Zhang, Y., et al., Allelic variation at the vernalization genes Vrn-A1, Vrn-B1, Vrn-D1, and Vrn-B3 in Chinese wheat cultivars and their association with growth habit, Crop Sci., 2008, vol. 48, no. 2, pp. 458–470.

    Article  CAS  Google Scholar 

  15. Potokina, E.K., Koshkin, V.A., Alekseeva, E.A., et al., Combinations of alleles of the Ppd and Vrn genes determine the heading time in common wheat varieties, Russ. J. Genet.: Appl. Res., 2012, vol. 2, no. 4, pp. 311–318.

    Article  Google Scholar 

  16. Zlotina, M.M., Kiseleva, A.A., and Potokina, E.K., Ispol’zovanie allel’-spetsifichnykh markerov genov Vrn i Ppd dlya ekspress-diagnostiki fotoperiodicheskoi chuvstvitel’nosti i potrebnosti v yarovizatsii myagkoi pshenitsy i yachmenya: Metodicheskie ukazaniya (The Use of Allel-Specific Markers of the Ppd and Vrn Genes for Express Diagnostics of Photoperiodic Sensitivity and Vernalization Requirements in Spring Bread Wheat), St. Petersburg: Vseross. Inst. Rastenievod., 2012.

    Google Scholar 

  17. Wilhelm, E., Turner, A., and Laurie, D., Photoperiod insensitive Ppd-A1a mutations in tetraploid wheat (Triticum durum Desf.), Theor. Appl. Genet., 2009, vol. 118, no. 2, pp. 285–294.

    Article  CAS  PubMed  Google Scholar 

  18. Nishida, H., Yoshida, T., Kawakami, K., et al., Structural variation in the 5’ upstream region of photoperiod-insensitive alleles Ppd-A1a and Ppd-B1a identified in hexaploid wheat (Triticum aestivum L.), and their effect on heading time, Mol. Breed., 2013, vol. 31, no. 1, pp. 27–37.

    Article  CAS  Google Scholar 

  19. Díaz, A., Zikhali, M., Turner, A., et al., Copy number variation affecting the Photoperiod-B1 and Vernalization-A1 genes is associated with altered flowering time in wheat (Triticum aestivum), PLoS One, 2012, vol. 7, no. 3, p. e33234.

    Article  PubMed Central  PubMed  Google Scholar 

  20. Ermilova, E.V., Zalutskaya, Zh.M., Lapina, T.V., and Matveeva, T.V., Kolichestvennyi analiz ekspressii genov (Quantitative Analysis of Gene Expression), St. Petersburg: TESSA, 2010.

    Google Scholar 

  21. Rebrikov, D.V., Samatov, G.A., Trofimov, D.Yu., et al., PTsR v real’nom vremeni (PCR in Real Time)), Moscow: BINOM. Laboratoriya znanii, 2009.

    Google Scholar 

  22. Koshkin, V.A., Potokina, E.K., Matvienko, I.I., and Eggi, E.E., Study of almost isogenic wheat lines that differ by photoperiod sensitivity genes, Mater. dokl. VII s’ezda o-va fiziologov rastenii Rossii (Proc. 7th Congress of the Society of Plant Physiologists of Russia), 2011, Part 1, pp. 373–377.

    Google Scholar 

  23. Ganal, M.W., Altmann, T., and Röber, M.S., SNP identification in crop plants, Curr. Opin. Plant Biol., 2009, vol. 12, no. 2, pp. 211–217.

    Article  CAS  PubMed  Google Scholar 

  24. Stelmakh, A.F., Genetic systems regulating flowering response in wheat, Euphytica, 1998, vol. 100, pp. 359–369.

    Article  Google Scholar 

  25. Goncharov, N.P., Genetics of growth habit (spring vs. winter) in common wheat: confirmation of the existence of dominant gene VRN4, Theor. Appl. Genet., 2003, vol. 107, no. 4, pp. 768–772.

    Article  CAS  PubMed  Google Scholar 

  26. Yan, L., Fu, D., Li, C., et al., The wheat and barley vernalization gene VRN3 is an orthologue of FT, Proc. Natl. Acad. Sci. U.S.A., 2006, vol. 103, no. 51, pp. 19581–19586.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  27. Yoshida, T., Nishida, H., Zhu, J., et al., Vrn-D4 is a vernalization gene located on the centromeric region of chromosome 5D in hexaploid wheat, Theor. Appl. Genet., 2010, vol. 120, pp. 543–552.

    Article  CAS  PubMed  Google Scholar 

  28. Worland, T. and Snape, J., Genetic basis of worldwide wheat varietal improvement, in The World Wheat Book: A History of Wheat Breeding, Paris: Lavoisier Publishing, 2001, pp. 61–67.

    Google Scholar 

  29. Trevaskis, B., The central role of the VERNALISATION1 gene in the vernalization response of cereals, Funct. Plant. Biol., 2010, vol. 37, pp. 479–487.

    Article  CAS  Google Scholar 

  30. Kumar, S., Sharma, V., Chaudhary, S., et al., Genetics of flowering time in bread wheat Triticum aestivum: complementary interaction between vernalizationinsensitive and photoperiod-insensitive mutations imparts very early flowering habit to spring wheat, J. Genet., 2012, vol. 91, no. 1, pp. 33–47.

    Article  PubMed  Google Scholar 

  31. Lysenko, N.S., Kiseleva, A.A., Mitrofanova, O.P., and Potokina, E.K., Katalog raionirovannykh sortov myagkoi pshenitsy iz kollektsii VIR s identifitsirovannymi allelyami Vrni Ppd-genov (Catalog of State Permitted Cultivars of Wheat in the All-Russian Research Institute of Plant Industry Collection with Identified Alleles of Vrn- and Ppd-Genes), St. Petersburg: Vseross. Inst. Rastenievod., 2014.

    Google Scholar 

  32. Seki, M., Chono, M., Matsunaka, H., et al., Distribution of photoperiod-insensitive alleles Ppd-B1a and Ppd-D1a and their effect on heading time in Japanese wheat cultivars, Breed. Sci., 2011, vol. 61, no. 4, p. 405.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Vavilov Research Institute of Plant Industry, St. Petersburg, 190000, Russia

    A. A. Kiseleva, E. E. Eggi, V. A. Koshkin & E. K. Potokina

  2. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russia

    E. A. Salina

  3. Department of General Genetics, Breeding, and Seed Growing, Berbekov Kabardino-Balkarian State University, Nalchik, 360000, Russia

    M. N. Sitnikov & M. Roder

Authors
  1. A. A. Kiseleva
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. E. Eggi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V. A. Koshkin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. N. Sitnikov
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. Roder
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. E. A. Salina
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. E. K. Potokina
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to E. K. Potokina.

Additional information

Original Russian Text © A.A. Kiseleva, E.E. Eggi, V.A. Koshkin, M.N. Sitnikov, M. Roder, E.A. Salina, E.K. Potokina, 2014, published in Genetika, 2014, Vol. 50, No. 7, pp. 802–813.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kiseleva, A.A., Eggi, E.E., Koshkin, V.A. et al. Detection of genetic determinants that define the difference in photoperiod sensitivity of Triticum aestivum L. near-isogenic lines. Russ J Genet 50, 701–711 (2014). https://doi.org/10.1134/S102279541405007X

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S102279541405007X

Keywords

Search

Navigation