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The Expression Potential of Novel Komagataella Strains

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Abstract

The expression potential of various strains from the Collection of the National Bio-Resource Center (BRC VKPM) belonging to the species Komagataella kurtzmanii, K. phaffii, and K. mondaviorum has been assessed by the level of production of the heterologous enzyme, Citrobacter freundii phytase. Heterologous expression in the K. mondaviorum strains was observed for the first time. We identified the strains of K. phaffii Y-4288, K. mondaviorum Y-4331 and K. phaffii Y-4287 species with a high level production of the heterologous enzyme, a high growth rate, the ability to accumulate a large amount of biomass, and moderate thermotolerance. It has been shown that the average productivity of the transformants based on K. phaffii Y-4288, K. mondaviorum Y-4331, and K. phaffii Y-4287 strains exceeds that of the commercial industrial recipient strain K. phaffii GS115 Y-2837 by more than 3, 5, and 6 times, respectively. The K. phaffii Y-4287 and K. mondaviorum Y-4331 strains exhibited moderate thermotolerance and the ability to accumulate a heterologous product at 37°C. The high expression potential of the identified strains opens up the possibility of creating recipient strains on their basis for high-level production of heterologous proteins.

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Funding

This work was supported by the Ministry of Science and Higher Education of the Russian Federation (grant no. 075-15-2019-1659 dated October 31, 2019) and was carried out using the resources of the Unique Scientific Facility of the All-Russian Collection of Industrial Microorganisms National Bio-Resource Center, Kurchatov Institute, GOSNIIGENETIKA NRC.

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Authors and Affiliations

  1. Kurchatov Institute National Research Center, 123182, Moscow, Russia

    T. L. Gordeeva, L. N. Borschevskaya, T. D. Feday & S. P. Sineoky

  2. Kurchatov Genomic Center, Kurchatov Institute National Research Center, GOSNIIGENETIKA, 117545, Moscow, Russia

    T. L. Gordeeva & L. N. Borschevskaya

  3. State Research Institute for Genetics and Selection of Industrial Microorganisms, Kurchatov Institute National Research Center, 117545, Moscow, Russia

    A. A. Tkachenko

Authors
  1. T. L. Gordeeva
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  2. L. N. Borschevskaya
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  3. T. D. Feday
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  4. A. A. Tkachenko
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  5. S. P. Sineoky
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Correspondence to T. L. Gordeeva.

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The authors declare that they have no conflicts of interest.

This article does not contain any studies involving animals performed by any of the authors.

This article does not contain any studies involving human participants performed by any of the authors outside the scope of people’s normal professional activities.

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Translated by I. Gordon

Abbreviations: CL, culture liquid; OD600, optical density at a wavelength of 600 nm; PCR, polymerase chain reaction; SDS-PAGE, polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate.

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Gordeeva, T.L., Borschevskaya, L.N., Feday, T.D. et al. The Expression Potential of Novel Komagataella Strains. Appl Biochem Microbiol 58, 916–922 (2022). https://doi.org/10.1134/S0003683822080038

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  • DOI: https://doi.org/10.1134/S0003683822080038

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