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Low-power infrared laser modulates mRNA levels from genes of base excision repair and genomic stabilization in heart tissue from an experimental model of acute lung injury

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Abstract

The aim of this study was to evaluate photobiomodulation effects on mRNA relative levels from genes of base excision repair and genomic stabilization in heart tissue from an experimental model of acute lung injury by sepsis. For experimental procedure, animals were randomly assigned to six main groups: (1) control group was animals treated with intraperitoneal saline solution; (2) LASER-10 was animals treated with intraperitoneal saline solution and exposed to an infrared laser at 10 J cm−2; (3) LASER-20 was animals treated with intraperitoneal saline solution and exposed to an infrared laser at 20 J cm−2; (4) acute lung injury (ALI) was animals treated with intraperitoneal LPS (10 mg kg−1); (5) ALI-LASER10 was animals treated with intraperitoneal LPS (10 mg kg−1) and, after 4 h, exposed to an infrared laser at 10 J cm−2 and (6) ALI-LASER20 was animals treated with intraperitoneal LPS (10 mg kg−1) and, after 4 h, exposed to an infrared laser at 20 J cm−2. Irradiation was performed only once and animal euthanasias for analysis of mRNA relative levels by RT-qPCR. Our results showed that there was a reduction of mRNA relative levels from ATM gene and an increase of mRNA relative levels from P53 gene in the heart of animals with ALI when compared to the control group. In addition, there was an increase of mRNA relative levels from OGG1 and APE1 gene in hearts from animals with ALI when compared to the control group. After irradiation, an increase of mRNA relative levels from ATM and OGG1 gene was observed at 20 J cm−2. In conclusion, low-power laser modulates the mRNA relative levels from genes of base excision repair and genomic stabilization in the experimental model of acute lung injury evaluated.

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Authors and Affiliations

  1. Mestrado Profissional em Diagnóstico em Medicina Veterinária, Pró Reitoria de Pesquisa e Pós Graduação, Universidade de Vassouras, Avenida Expedicionário Oswaldo de Almeida Ramos, 280, Vassouras, Rio de Janeiro, 27700000, Brazil

    Larissa Alexsandra da Silva Neto Trajano

  2. Departamento de Biofísica e Biometria, Instituto de Biologia Roberto Alcântara Gomes, Universidade do Estado do Rio de Janeiro, Avenida 28 de Setembro, 87, fundos, Vila Isabel, Rio de Janeiro, 20551030, Brazil

    Larissa Alexsandra da Silva Neto Trajano, Luiz Philippe da Silva Sergio, Diego Sá Leal de Oliveira, André Luiz Mencalha & Adenilson de Souza da Fonseca

  3. Mestrado Profissional em Ciências aplicadas em Saúde, Universidade de Vassouras, Avenida Expedicionário Oswaldo de Almeida Ramos, 280, Vassouras, Rio de Janeiro, 27700000, Brazil

    Larissa Alexsandra da Silva Neto Trajano, Eduardo Tavares Lima Trajano & Marco Aurélio dos Santos Silva

  4. Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Rua José Lourenço Khelmer-s/n, Campus Universitário, São Pedro, Juiz de Fora, Minas Gerais, 36036900, Brazil

    Flávia de Paoli

  5. Departamento de Ciências Fisiológicas, Instituto Biomédico, Universidade Federal do Estado do Rio de Janeiro, Rua Frei Caneca, 94, Rio de Janeiro, 20211040, Brazil

    Adenilson de Souza da Fonseca

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  1. Larissa Alexsandra da Silva Neto Trajano
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da Silva Neto Trajano, L.A., da Silva Sergio, L.P., de Oliveira, D.S.L. et al. Low-power infrared laser modulates mRNA levels from genes of base excision repair and genomic stabilization in heart tissue from an experimental model of acute lung injury. Photochem Photobiol Sci 21, 1299–1308 (2022). https://doi.org/10.1007/s43630-022-00221-3

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