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Metabolic engineering strategies for microbial utilization of C1 feedstocks

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Abstract

The use of abundant and cheap one carbon (C1) feedstocks to produce value-added chemicals is an important approach for achieving carbon neutrality and tackling environmental problems. The conversion of C1 feedstocks to high-value chemicals is dependent on efficient C1 assimilation pathways and microbial chassis adapted for efficient incorporation. Here, we opted to summarize the natural and synthetic C1 assimilation pathways and their key factors for metabolizing C1 feedstock. Accordingly, we discussed the metabolic engineering strategies for enabling the microbial utilization of C1 feedstocks for the bioproduction of value-added chemicals. In addition, we highlighted future perspectives of C1-based biomanufacturing for achieving a low-carbon footprint for the biosynthesis of chemicals.

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Acknowledgements

This study was supported by the Provincial Outstanding Youth Foundation of Jiangsu Province (BK20211529), the National Science Fund for Excellent Young Scholars (22122806) and the Fundamental Research Funds for the Central Universities (JUSRP22031).

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Authors and Affiliations

  1. State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, China

    Jian Zhang, Liang Guo, Cong Gao, Liming Liu & Xiulai Chen

  2. International Joint Laboratory On Food Safety, Jiangnan University, Wuxi, 214122, China

    Jian Zhang, Liang Guo, Cong Gao, Liming Liu & Xiulai Chen

  3. School of Life Sciences and Health Engineering, Jiangnan University, Wuxi, 214122, China

    Wei Song & Jing Wu

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  1. Jian Zhang
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  6. Liming Liu
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JZ and XLC collected the information and wrote the manuscript, XLC, LG, CG, WS, JW and LML revised the manuscript.

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Correspondence to Xiulai Chen.

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Zhang, J., Guo, L., Gao, C. et al. Metabolic engineering strategies for microbial utilization of C1 feedstocks. Syst Microbiol and Biomanuf 3, 122–136 (2023). https://doi.org/10.1007/s43393-022-00135-2

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