Abstract
In modern agricultural production, straw return (SR) plus nitrogen (N) fertilizer (SR-N) input has been considered a better practical mode to improve soil fertility and crop yield. Here, we investigate the underlying mechanisms of long-term SR-N application on wheat growth, yield, and quality. Wheat seeds were cultivated in 11-year-old plots treated with different N fertilizer levels under corn SR and non-straw return (NSR) conditions. Five levels of N fertilizer were set: N0 (0), N1 (90 kg ha−1), N2 (180 kg ha−1), N3 (270 kg ha−1), and N4 (360 kg ha−1). The wheat plants in SR-N2 exhibited the best growth status, the highest yield, and yield components. Physiological indicators exhibited that wheat plants in SR-N2 also had the maximum chlorophyll, soluble sugars, and protein contents, the highest antioxidant enzyme activities, and the lowest malondialdehyde (MDA) content. Wheat grains in SR-N2 had the highest protein content, which might be attributed to the highest glutamine synthetase activity during filling stage. qRT-PCR analysis showed that six genes related to N uptake and assimilation were significantly upregulated in SR-N2 treatment. Taken together, SR-N2 might be the effective mode that decrease the N fertilizer usage but maintain the maximize crop yield and grain quality by reducing oxidative stress and upregulating the expression of N metabolism-related genes.
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References
Arnon DI (1949) Copper enzymes in isolated chloroplasts: polyphenoloxidase in Beta vulgaris. Plant Physiol 24:1–15. https://doi.org/10.1104/pp.24.1.1
Bernard SM, Møller AL, Dionisio G, Kichey T, Jahn TP, Dubois F, Baudo M, Lopes MS, Tercé-Laforgue T, Foyer CH, Parry MA, Forde BG, Araus JL, Hirel B, Schjoerring JK, Habash DZ (2008) Gene expression, cellular localization and function of glutamine synthetase isozymes in wheat (Triticum aestivum L.). Plant Mol Biol 67:89–105. https://doi.org/10.1007/s11103-008-9303-y
Chen Y, Xin L, Liu J, Yuan M, Liu S, Jiang W (2017) Changes in bacterial community of soil induced by long-term straw returning. Sci Agric 74:349–356. https://doi.org/10.1590/1678-992x-2016-0025
Chen YF, Wang Y, Wu WH (2008) Membrane transporters for nitrogen, phosphate and potassium uptake in plants. J Integr Plant Biol 50:835–848. https://doi.org/10.1111/j.1744-7909.2008.00707.x
Chen Z, Wang H, Liu X, Liu Y, Gao S, Zhou J (2016) The effect of N fertilizer placement on the fate of urea-15N and yield of winter wheat in southeast China. PLoS ONE 11:e0153701. https://doi.org/10.1371/journal.pone.0153701
Gadaleta A, Nigro D, Giancaspro A, Blanco A (2011) The glutamine synthetase (GS2) genes in relation to grain protein content of durum wheat. Funct Integr Genomics 11:665–670. https://doi.org/10.1007/s10142-011-0235-2
Gong MB, Wang SJ, Li ZQ, Zhu PS, Ge HM, Gao JL, Song CY (2018) Wheat-corn cropping system with long-term total straw returning: effects of nitrogen application on growth, yield and quality of Winter Wheat. Chin Agric Sci Bull 34:7–14. https://doi.org/10.11924/j.issn.1000-6850.casb17090001
Hansen V, Muller-Stover D, Imparato V, Krogh PH, Jensen LS, Dolmer A (2017) The effects of straw or straw-derived gasification biochar applications on soil quality and crop productivity: a farm case study. J Environ Manage 186:88–95. https://doi.org/10.1016/j.jenvman.2016.10.041
Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198. https://doi.org/10.1016/0003-9861(68)90654-1
Hui Z, Tian FX, Wang GK, Wang GP, Wang W (2012) The antioxidative defense system is involved in the delayed senescence in a wheat mutant tasg1. Plant Cell Rep 31:1073–1084. https://doi.org/10.1007/s00299-012-1226-z
Jiang W, Li N, Zhang D, Meinhardt L, Cao B, Li Y, Song L (2020) Elevated temperature and drought stress significantly affect fruit quality and activity of anthocyanin-related enzymes in jujube (Ziziphus jujuba Mill. Cv. ‘Lingwuchangzao’). PLoS One 15:e0241491. https://doi.org/10.1371/journal.pone.0241491
Li H, Dai MW, Dai SL, Dong XJ (2018) Current status and environment impact of direct straw return in China’s cropland-a review. Ecotoxicol Environ Saf 189:293–300. https://doi.org/10.1016/j.ecoenv.2018.05.014
Li Y, Zhang H, Wang M, Chen S (2019) Diazotrophic Paenibacillus beijingensis BJ-18 provides nitrogen for plant and promotes plant growth, nitrogen uptake and metabolism. Front Microbiol 10:1119. https://doi.org/10.3389/fmicb.2019.01119
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real time quantitative PCR and the 2−ΔΔCT method. Methods 25:402–408. https://doi.org/10.1006/meth.2001.1262
Meng S, Zhang C, Su L, Li Y, Zhao Z (2016) Nitrogen uptake and metabolism of Populus simonii in response to PEG-induced drought stress. Environ Exp Bot 123:78–87. https://doi.org/10.1016/j.envexpbot.2015.11.005
Miller G, Suzuki N, Ciftci-Yilmaz S, Mittler R (2010) Reactive oxygen species homeostasis and signaling during drought and salinity stresses. Plant Cell Environ 33:453–467. https://doi.org/10.1111/j.1365-3040.2009.02041.x
Su Y, Lv JL, Yu M, Ma ZH, Xi H, Kou CL, He ZC, Shen AL (2020) Long-term decomposed straw return positively affects the soil microbial community. J Appl Microbiol 28:138–150. https://doi.org/10.1111/jam.14435
Tang L, Nie S, Li W, Fan C, Wang S, Wu F, Pan K (2019) Wheat straw increases the defense response and resistance of watermelon monoculture to Fusarium wilt. BMC Plant Biol 19:551. https://doi.org/10.1186/s12870-019-2134-y
Tilman D (1999) Global environmental impacts of agricultural expansion: the need for sustainable and efficient practices. Proc Nata Acad Sci USA 96:5995–6000. https://doi.org/10.1073/pnas.96.11.5995
Vitousek PM, Naylor R, Crews T, David MB, Drinkwater LE, Holland E, Johnes PJ, Katzenberger J, Martinelli LA, Matson PA, Nziguheba G, Ojima D, Palm CA, Robertson GP, Sanchez PA, Townsend AR, Zhang FS (2009) Nutrient imbalances in agricultural development. Science 324:1519–1520. https://doi.org/10.1126/science.1170261
Walsh OS, Shafian S, Christiaens RJ (2018) Nitrogen fertilizer management in dryland wheat cropping systems. Plants (basel) 7:9. https://doi.org/10.3390/plants7010009
Wen D, Xu H, Xie L, He M, Hou H, Wu C, Li Y, Zhang C (2018) Effects of nitrogen level during seed production on wheat seed vigor and seedling establishment at the transcriptome level. Int J Mol Sci 19:3417. https://doi.org/10.3390/ijms19113417
Wu PN, Wang YL, Li PF, Wang XN, Hou XQ (2019) Effects of straw returning combined with nitrogen fertilizer on spring maize yield and soil physicochemical properties under drip irrigation condition in Yellow River pumping irrigation area, Ningxia, China. Ying Yong Sheng Tai Xue Bao 30:4177–4185. https://doi.org/10.13287/j.1001-9332.201912.022
Xiong Q, Tang G, Zhong L, He H, Chen X (2018) Response to nitrogen deficiency and compensation on physiological characteristics, yield formation, and nitrogen utilization of rice. Front Plant Sci 9:1075. https://doi.org/10.3389/fpls.2018.01075
Yang SQ, Wang YS, Liu RL, Xing L, Yang ZL (2018) Improved crop yield and reduced nitrate nitrogen leaching with straw return in a rice-wheat rotation of Ningxia irrigation district. Sci Rep 8:9458. https://doi.org/10.1038/s41598-018-27776-5
Yang WZ, Yoon JM, Choi H, Fan YL, Chen RM, An G (2015) Transcriptome analysis of nitrogen-starvation-responsive genes in rice. BMC Plant Biol 15:31. https://doi.org/10.1186/s12870-015-0425-5
Zhang S, Yang C, Chen M, Chen J, Pan Y, Chen Y, Rahman SU, Fan J, Zhang Y (2019) Influence of nitrogen availability on Cd accumulation and acclimation strategy of Populus leaves under Cd exposure. Ecotoxicol Environ Saf 180:439–448. https://doi.org/10.1016/j.ecoenv.2019.05.031
Zhao Y, Wang M, Hu S, Zhang X, Zhu OY, Zhang G, Huang B, Zhao S, Wu J, Xie D, Zhu B, Yu D, Pan X, Xu S, Shi X (2018) Economics- and policy-driven organic carbon input enhancement dominates soil organic carbon accumulation in Chinese croplands. Proc Natl Acad Sci USA 115:4045–4050. https://doi.org/10.1073/pnas.1700292114
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This work was supported by the high-level talent fund from Qingdao Agricultural University (1115012) and the National Key Research and Development Program of China (2017YFD0301002).
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Table S1
Effects of different N fertilizer levels on grain yield of wheat in 2019. Different lowercase letters indicated significant difference in LSD test between treatments (P<0.05). TGW: thousand grain weight; SR: straw return; NSR: non-straw return; N0 (0), N1 (90 kg ha−1), N2 (180 kg ha−1), N3 (270 kg ha−1) and N4 (360 kg ha−1) refer to five N fertilizer levels designed, respectively (DOCX 17 KB)
Table S2
Primers used for qRT-PCR analysis (DOCX 15 KB)
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Zhu, K., Song, C., Liu, J. et al. Unravelling the Mechanisms of Improving Wheat Growth, Yield, and Grain Quality Under Long-Term Corn Straw Return plus N Fertilizer Mode. J Soil Sci Plant Nutr 21, 3428–3436 (2021). https://doi.org/10.1007/s42729-021-00617-7
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DOI: https://doi.org/10.1007/s42729-021-00617-7