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The antioxidative defense system is involved in the delayed senescence in a wheat mutant tasg1

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Abstract

Wheat, which is the most important food crop worldwide, is a cereal that presents considerable potential for increased yield. A new wheat (Triticum aestivum L.) mutant tasg1 with delayed leaf senescence was constructed using ethyl methane sulfonate as a mutagen. Natural senescence in tasg1 was distinctly delayed in the field, as indicated by the slower progression of chlorophyll degradation, net photosynthetic rate than its wild type. Further, the malondialdehyde and the hydrogen peroxide content was lower and antioxidative enzyme activity higher in tasg1 than those in its wild type during both natural senescence and methyl viologen-induced oxidative stress. The results suggest that tasg1 is a functional stay-green wheat mutant with the Type B (in which senescence initiates on schedule, but progresses at a rate lower than that in the respective WTs) or Type A (in which senescence initiates late but proceeds at a normal rate) and B combination and that the competence of the antioxidant defense system is one of the most important mechanisms underlying the expression of the stay-green phenotype.

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Abbreviations

APX:

Ascorbate peroxidase

CAT:

Catalase

Ci:

Intercellular CO2 concentration

DAA:

Days after anthesis

E:

Transpiration rate

EMS:

Ethyl methane sulfonate

Gs:

Stomatal conductance

H2O2 :

Hydrogen peroxide

MDA:

Malondialdehyde

MV:

Methyl viologen

PFD:

Photon flux density

Pn:

Net photosynthetic rate

POD:

Peroxidase

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (No. 30671259) and by the National Key technology R&D program (No. 2006BAD01A02-15).

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Authors and Affiliations

  1. State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Science, Shandong Agricultural University, Tai’an, Shandong, 271018, People’s Republic of China

    Zhen Hui, Feng-Xia Tian, Guo-kun Wang, Gui-Ping Wang & Wei Wang

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  1. Zhen Hui
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  2. Feng-Xia Tian
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  3. Guo-kun Wang
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  4. Gui-Ping Wang
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  5. Wei Wang
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Corresponding author

Correspondence to Wei Wang.

Additional information

Communicated by M. Jordan.

Z. Hui and F. Tian contributed equally to this paper.

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Supplementary material 1 (DOCX 20 kb)

299_2012_1226_MOESM2_ESM.tif

Supplementary material 2 (TIFF 3010 kb). Supplemental Fig. 1 Changes in leaf color of tasg1 (down) and the wild type (up) during dark-induced senescence. The detached leaves were transferred to complete darkness at 25°C for 8 days. Numbers indicate days after incubation. Bar = 1 cm

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Hui, Z., Tian, FX., Wang, Gk. et al. The antioxidative defense system is involved in the delayed senescence in a wheat mutant tasg1 . Plant Cell Rep 31, 1073–1084 (2012). https://doi.org/10.1007/s00299-012-1226-z

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  • DOI: https://doi.org/10.1007/s00299-012-1226-z

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