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The prospects for bioprinting tumor models: recent advances in their applications

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Abstract

Three-dimensional (3D) tumor models prepared from patient-derived cells have been reported to imitate some of the biological development processes of in situ tumors in vitro. These 3D tumor models share several important characteristics with their in vivo tumor counterparts. Accordingly, their applications in tumor modeling, drug screening, and precision-targeted treatment are promising. However, the establishment of tumor models is subject to several challenges, including advancements in scale size, repeatability, structural precision in time and space, vascularization, and the tumor microenvironment. Recently, bioprinting technologies enabling the editorial arrangement of cells, factors, and materials have improved the simulation of tumor models in vitro. Among the 3D bioprinted tumor models, the organoid model has been widely appreciated for its advantages of maintaining high heterogeneity and capacity for simulating the developmental process of tumor tissues. In this review, we outline approaches and potential prospects for tumor model bioprinting and discuss the existing bioprinting technologies and bioinks in tumor model construction. The multidisciplinary combination of tumor pathology, molecular biology, material science, and additive manufacturing will help overcome the barriers to tumor model construction by allowing consideration of the structural and functional characteristics of in vitro models and promoting the development of heterogeneous tumor precision therapies.

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Acknowledgements

The authors appreciated the financial support from the National Key R&D Program of China (No. 2018YFA0703000), the National Natural Science Foundation of China (No. 82072412), the Translation Medicine National Key Science and Technology Infrastructure (Shanghai) Open Project (No. TMSK-2020-118), the Lingang Laboratory “Seeking Outstanding Youth Program” Open Project (No. LG-QS-202206-04), and the Shanghai Municipal Natural Science Foundation (No. 19ZR1429100).

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  1. Changru Zhang, Xiaoqin Qiu, and Yu Dai have contributed equally to this work.

Authors and Affiliations

  1. School of Rehabilitation Medicine, Weifang Medical University, Weifang, 261041, China

    Changru Zhang & Jinwu Wang

  2. Engineering Research Center of Traditional Chinese Medicine Intelligent Rehabilitation, Ministry of Education, Institute of Rehabilitation Medicine, School of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Shanghai, 200120, China

    Hui Wang & Jinwu Wang

  3. Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China

    Changru Zhang, Yihao Liu, Haoyi Niu, Chengwei Wang, Kerong Dai & Jinwu Wang

  4. Cancer Prevention and Treatment Institute of Chengdu, Department of Oncology, Chengdu Fifth People’s Hospital (The Second Clinical Medical College, Affiliated Fifth People’s Hospital of Chengdu University of Traditional Chinese Medicine), Chengdu, 611130, China

    Xiaoqin Qiu

  5. Department of Pancreatic Surgery, West China Hospital, Sichuan University, Chengdu, 610041, China

    Yu Dai

  6. Department of Spinal Surgery, The Affiliated Hospital of Qingdao University, Qingdao, 266000, China

    Weiqing Kong & Xuelian Mi

  7. College of Medicine, Southwest Jiaotong University, Chengdu, 610036, China

    Ya Ren

  8. Med-X Research Institute, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China

    Han Yang

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  1. Changru Zhang
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  11. Han Yang
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Contributions

CRZ, XQQ, and YD involved in organizing the paper, formal analysis, conceptualization, writing the original draft, and writing commentary editing. WQK involved in collecting and organizing the paper and writing the first draft. YHL and XLM involved in writing the first draft and writing commentary editing. HYN and CWW involved in conceptualization and writing the original draft. HY involved in project management and fund raising. HW and YR involved in directing figure design and figure copyright acquisition. KRD and JWW reviewed and edited the final draft. All authors approved the manuscript.

Corresponding authors

Correspondence to Han Yang, Kerong Dai or Jinwu Wang.

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The authors declare that they have no conflict of interest.

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This study does not contain any studies with human or animal subjects performed by any of the authors.

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Zhang, C., Qiu, X., Dai, Y. et al. The prospects for bioprinting tumor models: recent advances in their applications. Bio-des. Manuf. 6, 661–675 (2023). https://doi.org/10.1007/s42242-023-00247-1

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