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Hyssopus officinalis exerts hypoglycemic effects on streptozotocin-induced diabetic rats via modulating GSK-3β, C-fos, NF-κB, ABCA1 and ABGA1 gene expression

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Abstract

Objectives

Type 2 diabetes mellitus (DMT2) is contributed to dual interactions between environmental factors and certain genetic factors. This impressed a great need for novel treatment strategy. Nevertheless, Hyssopus officinalis (H. officinalis) as a terrestrial herb is considered to be an important source of natural antioxidants, it could be assessed as an anti-hyperglycemic agent.

Methods

In the current study, HPLC identified the active constitutes of H. officinalis, including total polyphenols, and flavonoids. Type 2 diabetes mellitus was induced in male Wistar albino rats via a single ip dose of streptozotocin (STZ) (35 mg/kg BW). One week post diabetes induction, rats were administrated H. officinalis (500 mg/ kg BW) orally for one month. Molecular analysis was assessed to investigate the efficiency of H. officinalis on modulating ATP-binding cassette transporter A1 (ABCA1) and G1 (ABCG1) genes, in addition to apoptotic biomarkers, glycogen synthase kinase-3β (GSK-3β) and cellular oncogene-fos (C-fos) genes. Furthermore, inflammatory biomarkers, nuclear factor kappa-B (NF-κB) and tumor necrosis factor-α (TNF-α) gene expression were also assessed.

Results

H. officinalis alcoholic extract declared the presence of polyphenols as gallic acid and flavonoids as quercetin in addition to many active constituents. Apigenin-7-glucoside and Chlorgenic acid were the most common constituents in the extract. RT-PCR results declared a significant up-regulation in mRNA gene expression of ABCA1 and ABCG1 upon H. officinalis treatment. Meanwhile, C-fos gene expression recorded a slight down-regulation. Gene expression of apoptotic biomarker GSK-3β demonstrated a significant down regulation as well as inflammatory biomarkers NF-κB and TNF-α.

Conclusion

From the data recorded, it could be concluded that H. officinalis exerts a great hypoglycemic potential via modulating C-fos, GSK-3β, NF-κB, TNF-α, ABCA1 and ABCG1 gene expression and signaling pathways and could be considered as an effective candidate for DMT2 treatment.

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Acknowledgements

The authors are grateful to the National Research Centre for it’ssupport to complete this research.

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Authors and Affiliations

  1. Therapeutic Chemistry Department, Pharmaceutical and Drug Industries Division, National Research Center, El Buhouth St., Dokki, Cairo, 12622, Egypt

    Rehab M. Abdel-Megeed, Mai O. Kadry, Hassan Z. Ghanem & Abdel-Hamid Z. Abdel-Hamid

  2. Medicinal and Aromatic Plants Researches Department, Pharmaceutical and Drug Industries Division, National Research Centre, El Buhouth St., Dokki, Cairo, Egypt

    Samah A. El Newary & Hussein A. H. Said-Al Ahl

  3. Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Center, El Buhouth St., Dokki, Cairo, Egypt

    Rabeh A. El-Shesheny

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  1. Rehab M. Abdel-Megeed
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Abdel-Megeed, R.M., El Newary, S.A., Kadry, M.O. et al. Hyssopus officinalis exerts hypoglycemic effects on streptozotocin-induced diabetic rats via modulating GSK-3β, C-fos, NF-κB, ABCA1 and ABGA1 gene expression. J Diabetes Metab Disord 19, 483–491 (2020). https://doi.org/10.1007/s40200-020-00535-y

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