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Cyclic Stretch Effects on Adipose-Derived Stem Cell Stiffness, Morphology and Smooth Muscle Cell Gene Expression

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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

Recent investigations consider adipose-derived stem cells (ASCs) as a promising source of stem cells for clinical therapies. To obtain functional cells with enhanced cytoskeleton and aligned structure, mechanical stimuli are utilized during differentiation of stem cells to the target cells. Since function of muscle cells is associated with cytoskeleton, enhanced structure is especially essential for these cells when employed in tissue engineering. In this study by utilizing a custom-made device, effects of uniaxial tension (1Hz, 10% stretch) on cytoskeleton, cell alignment, cell elastic properties, and expression of smooth muscle cell (SMC) genes in ASCs are investigated. Due to proper availability of ASCs, results can be employed in cardiovascular engineering when production of functional SMCs in arterial reconstruction is required. Results demonstrated that cells were oriented after 24 hours of cyclic stretch with aligned pseudo-podia. Staining of actin filaments confirmed enhanced polymerization and alignment of stress fibers. Such phenomenon resulted in stiffening of cell body which was quantified by atomic force microscopy (AFM). Expression of SM α-actin and SM22 α-actin as SMC associated genes were increased after cyclic stretch while GAPDH was considered as internal control gene. Finally, it was concluded that application of cyclic stretch on ASCs assists differentiation to SMC and enhances functionality of cells.

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Authors and Affiliations

  1. Department of Biomedical Engineering, Faculty of Engineering, University of Isfahan, Daneshgah St., Azadi sq., Isfahan, 8174673441, Iran

    Mohsen Rabbani & Morteza Teymoori

  2. Faculty of Biomedical Engineering, Amirkabir University of Technology (Tehran Polytechnic), Hafez st., Tehran, 158754413, Iran

    Mohammad Tafazzoli-Shadpour

  3. National Cell Bank of Iran, Pasteur Institute of Iran, Farvardin 12st st., Tehran, 13164, Iran

    Mohammad Ali Shokrgozar

  4. Medical Nanotechnology and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Taleghani Hospital, Parvaneh St., Velenjak, Tehran, 1985717444, Iran

    Mohsen Janmaleki

Authors
  1. Mohsen Rabbani
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  2. Mohammad Tafazzoli-Shadpour
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  3. Mohammad Ali Shokrgozar
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  4. Mohsen Janmaleki
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Correspondence to Mohsen Rabbani.

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The authors have no financial conflicts of interest.

Ethical statement

Adipose tissue is excised from patients during orthopaedic knee surgery with informed consent considering ethical issues.

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Rabbani, M., Tafazzoli-Shadpour, M., Shokrgozar, M.A. et al. Cyclic Stretch Effects on Adipose-Derived Stem Cell Stiffness, Morphology and Smooth Muscle Cell Gene Expression. Tissue Eng Regen Med 14, 279–286 (2017). https://doi.org/10.1007/s13770-017-0033-6

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  • DOI: https://doi.org/10.1007/s13770-017-0033-6

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