Abstract
Genetic diversity analysis of 46 commercially prominent mandarins grown in different countries was carried out based on molecular and fruit traits. Out of 110 SSR markers, 64 polymorphic markers amplified a total of 155 alleles. Twenty SSR primers revealed 20 unique alleles which generated DNA fingerprints in 17 genotypes. The Polymorphism information content values ranged from 0.13 (CIBE 5156) to 0.73 (CCSM 40), with an average value of 0.45 across all genotypes. The diversity analysis illustrating the genetic relationships classified the genotypes into four major clusters. The ‘Nagpur’ and ‘Mudkhed’ mandarins in cluster I were closely related as these showed the highest genetic similarity having a similarity coefficient of 0.99. In fruit traits, higher heritability (h2) was observed in fruit weight while the higher genotypic coefficient of variation (GCV) was estimated for a number of seeds demonstrating the magnitude of genetic variability in a population.
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Acknowledgements
We would like to thank the Director, School of Agricultural Biotechnology, PAU, Ludhiana for providing the facilities to carry out the research. Also, the Department of Fruit Science, PAU, Ludhiana for providing the access to citrus germplasm.
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HK and RS performed the experiment, HK analyzed the data, and wrote the manuscript. GSS and GS designed the experiment. NKS revised the manuscript. All the authors read and approved the final manuscript.
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Kaur, H., Sidhu, G.S., Sarao, N.K. et al. Assessment of genetic diversity of mandarin cultivars grown in major citrus regions of world using morphological and microsatellite markers. Hortic. Environ. Biotechnol. 63, 425–437 (2022). https://doi.org/10.1007/s13580-021-00404-4
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DOI: https://doi.org/10.1007/s13580-021-00404-4