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Development and characterization of microsatellite markers in pomegranate (Punica granatum L.)

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Abstract

The pomegranate (Punica granatum L.) is a temperate climate species requiring high temperatures for proper and complete ripening. The species is consumed as a fresh fruit, but also can be used to obtain transformed products such as juice, jam, or preserve. It is a fruit tree species with a high degree of diversity, but the identification of cultivars by morphological traits is very difficult. Thus, the characterization of genotypes through molecular markers is of great value for germplasm preservation, genetic studies, and plant breeding. The number of simple sequence repeat (SSR or microsatellite) markers developed for this genus is too low, so in this work we report the development of 117 microsatellite loci from a CT/AG-enriched pomegranate genomic library. In order to check their utility, eleven accessions were analyzed. The polymorphism information content (PIC) value across all loci ranged between 0.09 and 0.71, with an average of 0.37. These markers will facilitate genetic diversity studies, mapping, and genotyping of pomegranate.

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Acknowledgments

We wish to thank Julian Bartual Martos from the ‘Estación Experimental Agraria’ of Elche for providing the plant material for the experiments.

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Authors and Affiliations

  1. Instituto Valenciano de Investigaciones Agrarias, carretera Moncada-Náquera Km 4,5 Moncada, Valencia, Spain

    José Miguel Soriano, Elena Zuriaga, Gerardo Llácer & Maria Luisa Badenes

  2. Departamento de Producción Agrícola, Universidad de Chile, Santa Rosa, 11315, Santiago, Chile

    Pía Rubio & Rodrigo Infante

Authors
  1. José Miguel Soriano
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  2. Elena Zuriaga
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  3. Pía Rubio
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  4. Gerardo Llácer
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  5. Rodrigo Infante
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  6. Maria Luisa Badenes
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Corresponding author

Correspondence to Maria Luisa Badenes.

Additional information

José Miguel Soriano and Elena Zuriaga contributed equally to this work.

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Soriano, J.M., Zuriaga, E., Rubio, P. et al. Development and characterization of microsatellite markers in pomegranate (Punica granatum L.). Mol Breeding 27, 119–128 (2011). https://doi.org/10.1007/s11032-010-9511-4

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  • DOI: https://doi.org/10.1007/s11032-010-9511-4

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