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Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production

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Abstract

This study aimed at the exploitation of lignocellulosic wastes for the evaluation of the newly isolated white-rot fungal strains enzymatic potential for bioethanol production. The isolates belonging to Basidiomycetes, Fomes fomentarius TMF2, Schizophyllum commune TMF3, and Bjerkandera adusta TMF1, could synthesize extracellular laccase and various hydrolase while growing on lignocellulosic waste materials. More specifically, for the first time, F. fomentarius TMF2 synthesized laccase using sunflower meal as a substrate. This substrate could stimulate B. adusta TMF1 for carboxymethyl cellulase and Avicelase production. The isolate B. adusta TMF1 was able to produce amylase during its growth on brewerʼs spent grain, which is up to now the best result reported for this activity of any B. adusta strain. Soybean meal was the most potent substrate for stimulating pectinase production by B. adusta TMF1 and S. commune TMF3. While growing on brewerʼs spent grain, B. adusta TMF1 and S. commune TMF3 produced high levels of xylanase. Spent coffee residues were for the first time tested as a substrate for hydrolase production by selected fungal species. Also, this is the first attempt where the produced enzymes by isolate B. adusta TMF1 were used for lignocellulose hydrolysis of brewerʼs spent grain and corn stover for bioethanol production, where under non-optimized conditions 0.94 g/L and 0.86 g/L of bioethanol could be produced, respectively. This study showed that novel white-rot fungal isolates, especially B. adusta TMF1, could grow on unexploited, low-cost lignocellulosic substrates and to produce biotechnological value-added products within environmental and economical accepted processes.

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Abbreviations

ANOVA:

Analysis of variance

BSG:

Brewerʼs spent grain

CMC:

Carboxymethyl cellulose

CTAB:

Cetyl trimethyl ammonium bromide

DNA:

Deoxyribonucleic acid

DNS method:

Dinitrosalicylic acid method

ITS:

Internal transcribed spacer

MEA:

Malt extract agar

NCBI:

The National Center for Biotechnology Information

PCR:

Polymerase chain reaction

rRNA:

Ribosomal ribonucleic acid

SBM:

Soybean meal

SCR:

Spent coffee residues

SFM:

Sunflower meal

SSF:

Solid-state fermentation

WRF:

White-rot fungi

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Acknowledgements

The authors would like to thank agricultural cooperative “Mrkšićevi salaši” for obtaining corn waste.

Funding

This work was supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia (Contract No. 451–03-68/2020–14/200135 and 451–03-9/2021–14/200287) and Bilateral Project Serbia/Croatia (337–00-205/2019–09/35) (2019–2021). Ministarstvo Prosvete,Nauke i Tehnološkog Razvoja,451–03-68/2020–14/200135,451–03-9/2021–14/200287,Bilateral Project Serbia/Croatia (337–00-205/2019–09/35) (2019–2021)

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Authors and Affiliations

  1. Innovation Centre of Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000, Belgrade, Serbia

    Nevena Ilić

  2. Department for Biochemical Engineering and Biotechnology, University of Belgrade, Faculty of Technology and Metallurgy, Karnegijeva 4, Belgrade, Serbia

    Slađana Davidović, Marija Milić, Mirjana Rajilić-Stojanović, Katarina Mihajlovski & Suzana Dimitrijević-Branković

  3. Department Medical College of Professional Health, Belgrade Academy of Professional Studies, Cara Dušana 254, Belgrade, Serbia

    Danijela Pecarski

  4. Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, Zagreb, Croatia

    Mirela Ivančić-Šantek

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  1. Nevena Ilić
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Contributions

N. Ilić: planning of study, investigations on isolation of fungi and determination of qualitative and quantitative enzymes activities, data analysis, results discussion, manuscript writing. S. Davidović: investigations, manuscript writing, performing statistics. M. Milić: investigations on fermentation processes, data analysis, results discussion, manuscript writing. M. Rajilić-Stojanović: molecular identification of fungal isolates, editing. Danijela Pecarski: investigations on fermentation processes. Mirela Ivančić-Šantek: investigations on bioethanol production. K. Mihajlovski: planning of study, investigations on bioethanol production, data analysis, results verification, manuscript writing. S. Dimitrijević-Branković: conceptualization, planning, and supervision.

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Correspondence to Nevena Ilić.

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Ilić, N., Davidović, S., Milić, M. et al. Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production. Biomass Conv. Bioref. 13, 17175–17186 (2023). https://doi.org/10.1007/s13399-021-02145-x

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